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厌氧氨氧化反应过程的三维荧光光谱解析

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刘怡心, 李卫华, 申慧彦, 张二杨. 厌氧氨氧化反应过程的三维荧光光谱解析[J]. 环境工程学报, 2015, 9(10): 4680-4686. doi: 10.12030/j.cjee.20151011
引用本文: 刘怡心, 李卫华, 申慧彦, 张二杨. 厌氧氨氧化反应过程的三维荧光光谱解析[J]. 环境工程学报, 2015, 9(10): 4680-4686. doi: 10.12030/j.cjee.20151011
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Liu Yixin, Li Weihua, Shen Huiyan, Zhang Eryang. Analysis of EEM fluorescence spectra of effluents from ANAMMOX reactor[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4680-4686. doi: 10.12030/j.cjee.20151011
Citation: Liu Yixin, Li Weihua, Shen Huiyan, Zhang Eryang. Analysis of EEM fluorescence spectra of effluents from ANAMMOX reactor[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4680-4686. doi: 10.12030/j.cjee.20151011
shu

厌氧氨氧化反应过程的三维荧光光谱解析

  • 1.

    安徽建筑大学环境与能源工程学院, 水污染控制与废水资源化安徽省重点实验室, 合肥 230601

  • 基金项目:

    国家自然科学基金资助项目(51378017)

    国家"水体污染控制与治理"科技重大专项(2012ZX07103-001)

    安徽省高校省级优秀青年人才基金重点项目(2013SQRL100ZD)

  • 中图分类号: X703

Analysis of EEM fluorescence spectra of effluents from ANAMMOX reactor

  • 1.

    School of Environment and Energy Engineering, Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Reuse, Anhui Jianzhu University, Hefei 230601, China

  • Fund Project:

  • 摘要: 采用三维荧光光谱表征了厌氧氨氧化UASB反应器启动过程中和有机冲击负荷条件下的出水样品,应用平行因子分析方法解析样品中各主成分的三维荧光光谱图。研究结果表明,反应器启动阶段,出水中含有类蛋白质、类富里酸和类腐殖酸物质,主要源于接种污泥的菌体自溶和残留有机物厌氧发酵。随着反应器的运行,出水中的类蛋白质,类富里酸物质和类腐殖酸荧光物质逐渐减少。在有机冲击负荷条件下,出水中的类蛋白质组分迅速增加并成为反应器出水中的主要荧光物质。该研究为厌氧氨氧化的启动及运行控制的监控表征提供了新的方法。
    Abstract: The effluents were characterized by excitation-emission matrix (EEM) fluorescence spectra from different operation conditions such as the start-up phase,and the organic loading shock phase.Parallel factor analysis was used to decompose the fluorescent components of the samples and their EEM fluorescence spectra were acquired.Results showed that there were protein-like,fulvic-like and humic-like substances in the effluents of the start-up phase,which was derived from the cell autolysis and anaerobic fermentation of inoculated sludge.With the operation of ANAMMOX reactor,the protein-like,the fulvic-like and humic-like substance decreased gradually.The protein-like substance increased rapidly and became the main fluorophore of the effluents under the organic shock loading conditions.This work provides a new monitoring method for the start-up and operational control of the ANAMMOX reactor.
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  • [1] Mulder A.,van de Graaf A.A.,Robertson L.A.,et al.Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor.FEMS Microbiology Ecology,1995,16(3):177-183
    [2] Van de Graaf A.A.,Mulder A.,de Bruijn P.,et al.Anaerobic oxidation of ammonium is a biologically mediated process.Applied Microbiology and Biotechnology,1995,61(4):1246-1251
    [3] Strous M.,Heijnen J.J.,Kuenen J.G.,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms.Applied Microbiology and Biotechnology,1998,50(5):589-596
    [4] 牟新利,高鹏,付川,等.三维荧光中药指纹图谱研究.光谱实验室,2010,27(1):180-183Mou Xinli,Gao Peng,Fu Chuan,et al.Study on fingerprint of traditional Chinese Medicine by three-dimensional fluorescence.Chinese Journal of Spectroscopy Laboratory,2010,27(1):180-183(in Chinese)
    [5] 任保卫,赵卫红,王江涛,等.海洋微藻生长过程藻液三维荧光特征.光谱学与光谱分析,2008,28(5):1130-1134Ren Baowei,Zhao Weihong,Wang Jiangtao,et al.Three dimensional fluorescence excitation-emission matrix spectrum of dissolved organic substance in marine microalgaes growth process.Spectroscopy and Spectral Analysis,2008,28(5):1130-1134(in Chinese)
    [6] 尹春丽,丁春晖,李华.昌黎原产地干红葡萄酒的三维荧光光谱特征研究.分析测试学报,2008,27(6):641-643,647Yin Chunli,Ding Chunhui,Li Hua.Study on the characteristics of three dimensional fluorescence spectra of Changli Dry Red Wines.Journal of Instrumental Analysis,2008,27(6):641-643,647(in Chinese)
    [7] 田广军,史锦珊.基于表观特征的三维荧光谱参量化油种鉴别.仪器仪表学报,2005,26(8):727-728,734Tian Guangjun,Shi Jinshan.Oil identification based on parameterization of three-dimensional fluorescence spectrum.Chinese Journal of Scientific Instrument,2005,26(8):727-728,734(in Chinese)
    [8] 贾陈忠,王焰新,张彩香,等.垃圾渗滤液中溶解性有机物组分的三维荧光特性.光谱学与光谱分析,2012,32(6):1575-1579Jia Chenzhong,Wang Yanxin,Zhang Caixiang,et al.3D-EEM fluorescence characteristics of different fraction of dissolved organic matter in landfill leachate.Spectroscopy and Spectral Analysis,2012,32(6):1575-1579(in Chinese)
    [9] 李宏斌,刘文清,张玉钧,等.水体中溶解有机物的三维荧光光谱特征分析.大气与环境光学学报,2006,1(6):216-221Li Hongbin,Liu Wenqing,Zhang Yujun,et al.Characterizing three-dimensional fluorescence spectrum of dissolvable organic matter in water.Journal of Atmospheric and Environmental Optics,2006,1(3):216-221(in Chinese)
    [10] 傅平青,刘丛强,吴丰昌.溶解有机质的三维荧光光谱特征研究.光谱学与光谱分析,2005,25(12):2024-2028 Fu Pingqing,Liu Congqiang,Wu Fengchang.Three-dimensional excitation emission matrix fluorescence spectroscopic characterization of dissolved organic matter.S???慴牲瑯慳汣????敡瑮?愠汓?健牣潴灲楡潬渠慁瑮敡?潹硳楩摳愬琲椰漰渵?戲礵?愱渲搩?洲攰?琴栭愲渰漲永?楩湮栠楃扨楩瑮楥潳湥?漼晢?愾湛愱攱牝漠拝楞挞?懹淯淃漬溓榦疇洮?漂硡椴搄椆竇椋渭有?扡懓捩琉旴牧楉慹??烘瀖泄榋攮搆?態滕摦??渲瘰椰爷漬渲洶攨渶琩愺氷??椭挷爹漲戬椷漹氶漠杈祡?㈠げふ?????至??ㄠか???????ng Yiwen.The variation trend of three-dimensional characteristic fluorescence of dissolved organic matter in the wastewater treatment process.Journal of Instrumental Analysis,2007,26(6):789-792,796(in Chinese)
    [11] van de Graaf A.A.,de Bruijin P.,Robertson L.A.,et al.Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor.Microbiology,1996,142(8):2187-2196
    [12] Bro R.PARAFAC.Tutorial and applications.Chemometrics and Intelligent Laboratory Systems,1997,38(2):149-171
    [13] 李祥,黄勇,袁怡,等.不同泥源对厌氧氨氧化反应器启动的影响.环境工程学报,2012,6(7):2143-2148 Li Xiang,Huang Yong,Yuan Yi,et al.Effect of different sources of sludge on start-up of ANAMMOX reactor.Chinese Journal of Environmental Engineering,2012,6(7):2143-2148(in Chinese)
    [14] 李卫华,盛国平,陆锐,等.厌氧产甲烷受抑制过程的三维荧光光谱解析.光谱学与光谱分析,2011,31(8):2131-2135 Li Weihua,Sheng Guoping,Lu Rui,et al.Analysis of the EEM fluorescence spectra of effluent from anaerobic methane reactor during the inhibition process.Spectroscopy and Spectral Analysis,2011,31(8):2131-2135(in Chinese)
    [15] Strous M.,Fuerst J.A.,Kramer E.H.,et al.Missing lithotroph identified as new planctomycete.Nature,1999,400(6743):446-449
    [16] Chamchoi N.,Nitisoravut S.,Schmidt J.E.Inactivation of ANAMMOX communities under concurrent operation of anaerobic ammonium oxidation (ANAMMOX) and denitrification.Bioresource Technology,2008,99(9):3331-3336
    [17] Tang Chongjian,Zheng Ping,Chai Liyuan,et al.Thermodynamic and kinetic investingation of anaerobic bioprocesses on ANAMMOX under high organic conditions.Chemical Engineering Journal,2013,230:149-157
    [18] 左宜,左剑恶,张薇.利用有机物厌氧发酵生物制氢的研究进展.环境科学与技术,2004,27(1):97-99,116 Zuo Yi,Zuo Jian'e,Zhang Wei.Review of research on biological hydrogen production by anaerobic fermentation of organic waste.Environmental Science & Technology,2004,27(1):97-99,116(in Chinese)
    [19] Güven D.,Dapena A
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  • 收稿日期:  2014-09-30
  • 刊出日期:  2015-10-14

厌氧氨氧化反应过程的三维荧光光谱解析

  • 1. 安徽建筑大学环境与能源工程学院, 水污染控制与废水资源化安徽省重点实验室, 合肥 230601
  • 收稿日期:  2014-09-30
基金项目:

国家自然科学基金资助项目(51378017)

国家"水体污染控制与治理"科技重大专项(2012ZX07103-001)

安徽省高校省级优秀青年人才基金重点项目(2013SQRL100ZD)

摘要: 采用三维荧光光谱表征了厌氧氨氧化UASB反应器启动过程中和有机冲击负荷条件下的出水样品,应用平行因子分析方法解析样品中各主成分的三维荧光光谱图。研究结果表明,反应器启动阶段,出水中含有类蛋白质、类富里酸和类腐殖酸物质,主要源于接种污泥的菌体自溶和残留有机物厌氧发酵。随着反应器的运行,出水中的类蛋白质,类富里酸物质和类腐殖酸荧光物质逐渐减少。在有机冲击负荷条件下,出水中的类蛋白质组分迅速增加并成为反应器出水中的主要荧光物质。该研究为厌氧氨氧化的启动及运行控制的监控表征提供了新的方法。

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