高级搜索

生活污水中肠道病毒分布特性的定量PCR研究

,  ,  , 

downloadPDF

上一篇

下一篇

周进宏, 王晓昌, 张崇淼, 徐丽梅. 生活污水中肠道病毒分布特性的定量PCR研究[J]. 环境工程学报, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
引用本文: 周进宏, 王晓昌, 张崇淼, 徐丽梅. 生活污水中肠道病毒分布特性的定量PCR研究[J]. 环境工程学报, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
shu
Zhou Jinhong, Wang Xiaochang, Zhang Chongmiao, Xu Limei. Study on distribution characteristic of enteroviruses in domestic wastewater by quantitative real-time PCR method[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
Citation: Zhou Jinhong, Wang Xiaochang, Zhang Chongmiao, Xu Limei. Study on distribution characteristic of enteroviruses in domestic wastewater by quantitative real-time PCR method[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
shu

生活污水中肠道病毒分布特性的定量PCR研究

  • 1.

    西安建筑科技大学环境与市政工程学院, 西安 710055

  • 基金项目:

    国家自然科学基金重大国际合作项目(51021140002)

    西安市科技计划项目(CX12160)

  • 中图分类号: X172

Study on distribution characteristic of enteroviruses in domestic wastewater by quantitative real-time PCR method

  • 1.

    School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

  • Fund Project:

  • 摘要: 为了明确污水中肠道病毒的分布及去除规律,采用定量PCR方法对西安市3个污水厂进行了为期一年的监测。结果表明,肠道病毒和人类肠道病毒在进水和二级出水中呈较高浓度,约分布在102~105 copies/mL和102~104 copies/mL范围内。肠道病毒和人类肠道病毒浓度呈现季节性变化,秋季浓度最高,冬季次之,春夏最低。经统计学分析发现,肠道病毒和人类肠道病毒浓度在3个水厂间无显著差异性(p<0.05)且肠道病毒和人类肠道病毒很好地服从对数正态分布。通过病毒的检测数据及分布特性,明确了函数参数并确定人类肠道病毒占肠道病毒的39%。经过常规二级处理,肠道病毒可去除1.12 log左右,与人类肠道病毒去除率相近,且不同水厂对病毒的去除率也相近。
    Abstract: In this 1-year study, real-time PCR was conducted using samples from 3 domestic wastewater treatment plants (WWTPs) in Xi'an, China, to investigate the distribution of enteroviruses and human enteroviruses in domestic wastewater and to determine the behavior of these viruses after wastewater treatment. Before disinfection, the load of enteroviruses and human enteroviruses in the 3 WWTPs ranged from 102 to 105 copies/mL in influents and from 102 to 104 copies/mL in effluents. Further, enteroviruses and human enteroviruses showed seasonal distribution in the influents, with the highest distribution recorded in autumn, followed by winter, and the lowest distribution recorded in spring and summer. Statistical analysis showed that the load of enteroviruses and human enteroviruses in the 3 WWTPs was not significantly different (p1.12 log of the enteroviruses, which was similar to the removal efficiency for human enteroviruses. Moreover, removal of enteroviruses and human enteroviruses from the 3 WWTPs was similar.
  • 加载中
  • [1] Fattal B., Vasl R. J., Katzenelson E., et al. Survival of bacterial indicator organisms and enteric viruses in the Mediterranean coastal waters off Tel-Aviv. Water Research, 1983, 17(4):397-402
    [2] Gilgen M., Germann D., Lüthy J., et al. Three-step isolation method for sensitive detection of enterovirus, rotavirus, hepatitis A virus, and small round structured viruses in water samples. International Journal of Food Microbiology, 1997, 37(2-3):189-199
    [3] Walters S. P., Yamahara K. M., Boehm A. B. Persistence of nucleic acid markers of health-relevant organisms in seawater microcosms:Implications for their use in assessing risk in recreational waters. Water Reseach, 2009, 43(19):4929-4939
    [4] Usha D., Rishi S. Detection of water-borne pathogens:Culture plate to genomics. Indian Journal of Science and Technology, 2009, 2(11):59-71
    [5] Steyer A., Torkar K. G., Gutiérrez-Aguirre I., et al. High prevalence of enteric viruses in untreated individual drinking water sources and surface water in Slovenia. International Journal of Hygiene and Environmental Health, 2011, 214(5):392-398
    [6] Savichtcheva O., Okabe S. Alternative indicators of fecal pollution:Relations with pathogens and conventional indicators, current methodologies for direct pathogen monitoring and future application perspectives. Water Research, 2006, 40(13):2463-2476
    [7] Simmons F. J., Xagoraraki I. Release of infectious human enteric viruses by full-scale wastewater utilities. Water Research, 2011, 45(12):3590-3598
    [8] Redwan N. A., Attar R. M. Rotavirus infection and its monitoring in waste water using RT-PCR in Jeddah, Saudi Arabia. International Research Journal of Microbiology, 2012, 3(3):94-100
    [9] Fernández M. D. B., Torres C., Poma H. R., et al. Environmental surveillance of norovirus in Argentina revealed distinct viral diversity patterns, seasonality and spatio-temporal diffusion processes. Science of the Total Environment, 2012, 437:262-269
    [10] Wyn-Jones A. P., Carducci A., Cook N., et al. Surveillance of adenoviruses and noroviruses in European recreational waters. Water Research, 2011, 45(3):1025-1038
    [11] Francy D. S., Stelzer E. A., Bushon R. N., et al. Comparative effectiveness of membrane bioreactors, conventional secondary treatment, and chlorine and UV disinfection to remove microorganisms from municipal wastewaters. Water Research, 2012, 46(13):4164-4178
    [12] Rezaeinejad S., Vergara G. G. R. V., Woo C. H., et al. Surveillance of enteric viruses and coliphages in a tropical urban catchment. Water Research, 2014, 58:122-131
    [13] Casas N., Suñén E. Detection of enteroviruses, hepatitis A virus and rotaviruses in sewage by means of an immunomagnetic capture reverse transcription-PCR assay. Microbiological Reseach, 2002, 157(3):169-176
    [14] Bosch A., Guix S., Sano D., et al. New tools for the study and direct surveillance of viral pathogens in water. Current Opinion in Biotechnology, 2008, 19(3):295-301
    [15] Lewis G. D., Metcaif T. G. Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples. Applied and Environmental Microbiology, 1988, 54(8):1983-1988
    [16] Rabenau H. F., Clarici A. M. K., Mühlbauer G., et al. Rapid detection of enterovirus infection by automated RNA extraction and real-time fluorescence PCR. Journal of Clinical Virology, 2002, 25(2):155-164
    [17] Zhang Chongmiao, Wang Xiaochang, Liu Yongjun, et al. Simultaneous detection of enteroviruses from surface waters by real-time RT-PCR with universal primers. Journal of Environmental Sciences, 2010, 22(8):1261-1266
    [18] Katayama H., Haramoto E., Oguma K., et al. One-year monthly quantitative survey of noroviruses, enteroviruses, and adenoviruses in wastewater collected from six plants in Japan. Water Research, 2008, 42(6-7):1441-1448
    [19] Skraber S., Ogorzaly L., Helmi K., et al. Occurrence and persistence of enteroviruses, noroviruses and F-specific RNA phages in natural wastewater biofilms. Water Research, 2009, 43(19):4780-4789
    [20] Hewitt J., Leonard M., Greening G. E., et al. Influence of wastewater treatment process and the population size on human virus profiles in wastewater. Water Research, 2011, 45(18):6267-6276
    [21] Schvoerer E., Ventura M., Dubos O., et al. Qualitative and quantitative molecular detection of enteroviruses in water from bathing areas and from a sewage treatment plant. Research in Microbiology, 2001, 152(2):179-186
    [22] Rosa G. L., Pourshaban M., Iaconelli M., et al. Quantitative real-time PCR of enteric viruses in influent and effluent samples from wastewater treatment plants in Italy. Ann 1st Super Sanita, 2010, 46(3):266-273
    [23] 明红霞, 樊景风, 朱琳. 渤海湾肠道病毒的季节分布及其污染类型分析. 海洋学报, 2013, 35(6):162-169 Ming Hongxia, Fan Jingfeng, Zhu Lin. Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay. Acta Oceanologica Sinica, 2013, 35(6):162-169(in Chinese)
    [24] Hofstra N. Quantifying the impact of climate change on enteric waterborne pathogen concentrations in surface water. Current Opinion in Environmental Sustainability, 2011, 3(6):471-479
    [25] Kistemann T., Rind E., Rechenburg A., et al. A comparison of efficiencies of microbiological pollution removal in six sewage treatment plants with different treatment systems. International Journal of Hygiene and Environmental Health, 2008, 211(5-6):534-545
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1967
  • HTML全文浏览数:  1439
  • PDF下载数:  577
  • 施引文献:  0
出版历程
  • 收稿日期:  2015-01-02
  • 刊出日期:  2016-03-18
周进宏, 王晓昌, 张崇淼, 徐丽梅. 生活污水中肠道病毒分布特性的定量PCR研究[J]. 环境工程学报, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
引用本文: 周进宏, 王晓昌, 张崇淼, 徐丽梅. 生活污水中肠道病毒分布特性的定量PCR研究[J]. 环境工程学报, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
shu
Zhou Jinhong, Wang Xiaochang, Zhang Chongmiao, Xu Limei. Study on distribution characteristic of enteroviruses in domestic wastewater by quantitative real-time PCR method[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
Citation: Zhou Jinhong, Wang Xiaochang, Zhang Chongmiao, Xu Limei. Study on distribution characteristic of enteroviruses in domestic wastewater by quantitative real-time PCR method[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1023-1029. doi: 10.12030/j.cjee.20160302
shu

生活污水中肠道病毒分布特性的定量PCR研究

  • 1. 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 收稿日期:  2015-01-02
基金项目:

国家自然科学基金重大国际合作项目(51021140002)

西安市科技计划项目(CX12160)

摘要: 为了明确污水中肠道病毒的分布及去除规律,采用定量PCR方法对西安市3个污水厂进行了为期一年的监测。结果表明,肠道病毒和人类肠道病毒在进水和二级出水中呈较高浓度,约分布在102~105 copies/mL和102~104 copies/mL范围内。肠道病毒和人类肠道病毒浓度呈现季节性变化,秋季浓度最高,冬季次之,春夏最低。经统计学分析发现,肠道病毒和人类肠道病毒浓度在3个水厂间无显著差异性(p<0.05)且肠道病毒和人类肠道病毒很好地服从对数正态分布。通过病毒的检测数据及分布特性,明确了函数参数并确定人类肠道病毒占肠道病毒的39%。经过常规二级处理,肠道病毒可去除1.12 log左右,与人类肠道病毒去除率相近,且不同水厂对病毒的去除率也相近。

English Abstract

    全文HTML

参考文献 (25)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心