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喹诺酮抗性基因在城市污水处理系统中的分布及去除

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李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
引用本文: 李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu
Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
Citation: Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu

喹诺酮抗性基因在城市污水处理系统中的分布及去除

  • 1.

    石河子大学化学化工学院／新疆兵团化工绿色过程重点实验室, 石河子 832000

  • 基金项目:

    国家自然科学基金资助项目(21267019)

  • 中图分类号: X703

Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant

  • 1.

    Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China

  • Fund Project:

  • 摘要: 城市污水处理厂是耐药细菌和抗生素抗性基因进入自然环境的主要途径之一。对污水处理厂不同工艺采集7组样品,重点考察污水处理系统中喹诺酮类耐药细菌和7种喹诺酮类抗性基因污染分布及其去除效率。结果显示,在不同处理工艺阶段均检测到耐环丙沙星细菌,最高浓度为(6.65±0.19)×106 CFU/mL,耐药率为0.87%~7.24%。污水处理系统受抗生素抗性基因严重污染,入水样品中抗性基因总浓度为(1.28±0.12)×107 copies/mL。污水处理工艺对抗环丙沙星细菌和喹诺酮类抗性基因具有有效的去除作用,除qnrC去除效率为75%,其余均在98%以上,但是仍有浓度为2.77×104 CFU/mL的耐环丙沙星细菌和3.98×104 copies/mL的抗性基因进入自然环境。表明污水处理系统已成为耐药细菌和抗生素抗性基因的储存库和污染源。
    Abstract: The municipal sewage treatment plant is a major route through which antibiotic bacteria as well as antibiotic-resistance genes from anthropogenic sources are introduced into the natural ecosystems. In the present study, wastewater samples were collected from a sequence of process stages in the municipal sewage treatment plant. The concentrations of quinolone-resistant bacteria and quinolone-resistance genes were investigated, and their removal efficiency was presented in a preliminary analysis. Ciprofloxacin-resistant bacteria were detected in all treatment processes of the sewage treatment plant, and the highest concentration was found to be (6.65±0.19)×106 CFU/mL in the influent. The rates of ciprofloxacin-resistant bacteria made up 0.87% to 7.24% of total bacteria. It was observed that quinolone-resistance genes were prevalent in all the samples from the sewage treatment plant, except for the qnrB gene. The total concentration of the quinolone-resistance genes was (1.28±0.12)×107 copies/mL in the influent from the sewage treatment processes. The ciprofloxacin-resistant bacteria and quinolone-resistance genes were effectively removed during the sewage treatment processes, and their removal efficiency was more than 98%, except for the qnrC gene. However, a high abundance of ciprofloxacin-esistant bacteria and quinolone-resistance genes were released in the natural environment. This indicates that the sewage treatment systems have become reservoirs and contamination sources for the antibiotic-resistant bacteria and antibiotic-resistance genes.
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  • 收稿日期:  2015-10-14
  • 刊出日期:  2016-03-18
李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
引用本文: 李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu
Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
Citation: Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu

喹诺酮抗性基因在城市污水处理系统中的分布及去除

  • 1. 石河子大学化学化工学院／新疆兵团化工绿色过程重点实验室, 石河子 832000
  • 收稿日期:  2015-10-14
基金项目:

国家自然科学基金资助项目(21267019)

摘要: 城市污水处理厂是耐药细菌和抗生素抗性基因进入自然环境的主要途径之一。对污水处理厂不同工艺采集7组样品,重点考察污水处理系统中喹诺酮类耐药细菌和7种喹诺酮类抗性基因污染分布及其去除效率。结果显示,在不同处理工艺阶段均检测到耐环丙沙星细菌,最高浓度为(6.65±0.19)×106 CFU/mL,耐药率为0.87%~7.24%。污水处理系统受抗生素抗性基因严重污染,入水样品中抗性基因总浓度为(1.28±0.12)×107 copies/mL。污水处理工艺对抗环丙沙星细菌和喹诺酮类抗性基因具有有效的去除作用,除qnrC去除效率为75%,其余均在98%以上,但是仍有浓度为2.77×104 CFU/mL的耐环丙沙星细菌和3.98×104 copies/mL的抗性基因进入自然环境。表明污水处理系统已成为耐药细菌和抗生素抗性基因的储存库和污染源。

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