摘要:
通过盆栽试验,评价土壤长残效期除草剂氯磺隆与重金属镉单一污染及复合污染对后茬作物菠菜生长、代谢产物的影响。基于GC-MS技术对3个不同处理组菠菜的代谢产物进行了辨识和分析。主成分分析结果表明,3个处理组的代谢物含量存在显著差异。具体表现为:1)与空白对照相比,氯磺隆诱导菠菜多种氨基酸、与能量代谢相关的3种糖类物质、苹果酸、γ-氨基丁酸、乳酸和腐胺的含量显著上升(*P<0.05);肌醇显著下降(*P<0.05);镉诱导菠菜多种氨基酸、与能量代谢相关的5种糖类物质、参与三羧酸循环的苹果酸和柠檬酸含量显著上升(*P<0.05);肌醇和腐胺含量显著下降(*P<0.05)。2)与单一污染相比较,复合污染诱导菠菜多种氨基酸,多种糖类物质和苹果酸等含量显著下降(*P<0.05)。由此可见,复合污染减弱了氯磺隆和镉单一污染时对氨基酸代谢和能量代谢的上调作用;氯磺隆和镉之间没有协同作用,相反对某些特定的代谢物存在拮抗作用。
关键词:
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氯磺隆
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镉
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菠菜
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代谢物
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GC-MS
Abstract:
The single and combined stress effects of the long residual herbicide chlorsulfuron and cadmium were evaluated on the growth and metabolites of Spinacia oleracea L. in this study. The metabolites of Spinacia oleracea L. in different treatment groups were identified and analyzed by GC-MS technique. Combined with the results from the control group, there were individually significant differences in the detection of metabolites from the three treatment groups. 1) Chlorsulfuron prompted a large increase (*P<0.05) in the content of multiple-amino acids, the three carbohydrates involved in energy metabolism, malic acid, γ-aminobutyric acid (GABA), lactic acid and putrescine, otherwise, an obvious decrease of inositol (*P<0.05). In the other hand, cadmium caused an increase (*P<0.05) in multiple-amino acids, five energy-metabolism-related carbohydrates, malic acid and citric acid, however a decrease (*P<0.05) of putrescine and inositol. 2) Unlike the results of single treatment group of chlorsulfuron or Cd, the detection in combination treatment group showed a decrease (*P<0.05) in multiple-amino acids, multiple-carbohydrates and malic acid and et al. Summarizing the results mentioned above, we concluded that the combined effects by soil residual chlorsulfuron and cadmium could obviously impair the up-effects on the amino acids and energy metabolisms induced by any single one of them; in addition, the combination effects by chlorsulfuron and cadmium also did not demonstrate an synergy but an antagonism on some metabolites.
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