电化学法检测芴的细胞毒性
Cytotoxicity of Fluorene Assayed by Electrochemistry
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摘要: 以小鼠胚胎成纤维细胞（BALB/c 3T3）和中国仓鼠肺细胞（V79）为模型细胞，利用基于石墨烯量子点/玻碳电极（RGOQDs/GCE）的细胞电化学法研究多环芳烃（PAHs）类化合物芴的细胞毒性，并与四唑盐比色试验（MTT）对比，验证电化学法的可靠性。时间-效应研究结果表明，培养时间为30 h时，芴对2种细胞的毒性最强；剂量-效应研究结果表明，电化学法测得的芴对BALB/c 3T3和V79细胞毒性的半数抑制浓度（IC50）值分别为0.89 mmol·L-1和0.25 mmol·L-1，MTT法测得的IC50值分别为1.34 mmol·L-1和0.86 mmol·L-1。这说明芴对V79细胞的毒性效应更强，这可能是由于芴对V79细胞嘌呤核苷酸代谢影响更大。电化学法的检测结果与MTT法的结果趋势一致，且IC50值均低于MTT法，说明本文使用的电化学法可以有效灵敏地评价芴的细胞毒性。芴的细胞毒性作用机制可能是通过激活细胞中p53基因进而影响细胞嘌呤核苷酸代谢。本法通过检测细胞中嘌呤含量变化而评价芴的细胞毒性。Abstract: Mouse embryonic fibroblasts (BALB/c 3T3) and Chinese hamster lung cells (V79) were used as model cells. Electrochemical methods based on graphene quantum dots/glassy carbon electrode (RGOQDs/GCE) were used to study the cytotoxicity of fluorene on BALB/c 3T3 and V79 cells, which were verified by MTT assay. The results of time-effect showed that the optimal action time of fluorene on BALB/c 3T3 and V79 cells was 30 h. The results of dose-response showed that the median inhibition concentration (IC50) values of fluorene on BALB/c 3T3 and V79 cells measured by electrochemical method were 0.89 mmol·L-1 and 0.25 mmol·L-1, respectively. And the IC50 values measured by MTT method were 1.34 mmol·L-1 and 0.86 mmol·L-1, respectively. It was suggested that fluorene has stronger toxic effect on V79 cells, which may be due to the greater effect of fluorene on purine nucleotide metabolism of V79 cells. The results of electrochemical method were consistent with the results of MTT method, and IC50 values were lower than that of MTT method, indicating that the electrochemical method used in this paper can evaluate the cytotoxicity of fluorene effectively and sensitively. The cytotoxicity mechanism may be that p53 gene was activated in cells under the action of fluorene and then affected purine nucleotide metabolism. The cytotoxicity of fluorene could be evaluated by the change of purine content in cells.
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Key words:
- fluorene /
- BALB/c 3T3 /
- V79 /
- cytotoxicity /
- purine nucleotide /
- electrochemistry
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