菲对泥鳅(Misgurnus anguillicaudatus)肝性腺指数和卵黄蛋白原转录水平的影响
Effects of Phenanthrene on Hepatic Gonadal Index and Vitellogenin Gene Expression in Misgurnus anguillicaudatus
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摘要: 为探究菲（phenanthrene，PHE）对鱼类的内分泌干扰效应及生殖毒性损伤，将泥鳅（Misgurnus anguillicaudatus）暴露于菲浓度为1.33、1.77、2.36和3.13 mg·L-1的水体中28 d，分别于7、14和28 d取样，采用称重法测定肝脏指数、性腺指数；qRT-PCR法检测卵黄蛋白原基因（vtg1、vtgc）在泥鳅肝脏和性腺的转录水平；酶联免疫吸附法测定卵黄蛋白原（vitellogenin，VTG）含量。结果显示：（1） PHE胁迫使雄性泥鳅肝脏指数升高、性腺指数下降；高浓度PHE胁迫引起雌性泥鳅肝脏指数上升，性腺指数升高；（2） PHE胁迫导致泥鳅vtg1和vtgc表达量显著升高，雄性泥鳅vtg1和vtgc转录水平与菲胁迫浓度之间呈现低浓度诱导、高浓度抑制的趋势；（3） PHE暴露使泥鳅肝脏、性腺VTG含量显著升高，且在PHE较高浓度组中尤为明显。这表明，一定浓度PHE暴露可以导致泥鳅肝脏和性腺发育异常；较低浓度PHE暴露可诱导肝脏和性腺vtg1、vtgc转录水平的表达，而高浓度PHE则抑制上述基因表达，PHE胁迫引起vtg1和vtgc表达水平存在差异；PHE可以显著诱导肝脏和性腺VTG的产生。因此，高浓度PHE干扰了泥鳅的性腺作用轴。Abstract: To study the effects of phenanthrene (PHE) on fish endocrine disruption and reproductive toxicity, loach (Misgurnus anguillicaudatus) was exposed to 1.33, 1.77, 2.36 and 3.13 mg·L-1 for 28 d. During the PHE treatment, the liver and gonad tissues of loach were collected on 7, 14 and 28 d. The hepatosomatic index (HSI) and gonadosomatic index (GSI) were measured by weighing method, the expression of vitellogenin 1 (vtg1) and vitellogenin c (vtgc) in the liver and gonad of M. anguillicaudatus was detected by qRT-PCR, and the content of vitellogenin (VTG) was determined by enzyme linked immunosorbent assay (ELISA). The results showed that:(1) PHE stress significantly increased HSI and decreased GSI of male, and significantly increased HSI and GSI of female. (2) PHE stress caused significant increase of vtg1 and vtgc expression in M. anguillicaudatus, and the expression of vtg1 and vtgc in male M. anguillicaudatus showed a trend of low concentration induction and high concentration inhibition. (3) PHE exposure significantly increased VTG content in liver and gonad of M. anguillicaudatus, especially in the group with higher concentration of PHE. It is suggested that certain concentration of PHE could induce abnormal development of liver and gonad of M. anguillicaudatus. Additionally, the lower concentration of PHE could induce expression of vtg1 and vtgc in liver and gonad, while the higher concentration of PHE could inhibit the expression of those two genes, and the expression of vtg1 and vtgc were different under PHE stress. Moreover, PHE could induce the production of VTG in liver and gonad of loach. Therefore, the gonadal axis of M. anguillicaudatus was disturbed by PHE contamination.
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Key words:
- phenanthrene /
- Misgurnus anguillicaudatus /
- chronic toxicity /
- vitellogenin
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李爽, 刘殷佐, 刘入瑜, 等. 浑河沈抚段多环芳烃的污染特征及风险评价[J]. 中国环境科学, 2019, 39(4):1551-1559 Li S, Liu Y Z, Liu R Y, et al. The pollution characteristics and risk assessment of polycyclic aromatic hydrocarbons of Shen-Fu section of the Hun River Basin[J]. China Environmental Science, 2019, 39(4):1551-1559(in Chinese)
de Campos M F, Lo Nostro F L, Da Cuña R H, et al. Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae:Perciformes)[J]. General and Comparative Endocrinology, 2018, 257:255-263 范博, 王晓南, 黄云, 等. 我国七大流域水体多环芳烃的分布特征及风险评价[J]. 环境科学, 2019, 40(5):2101-2114 Fan B, Wang X N, Huang Y, et al. Distribution and risk assessment of polycyclic aromatic hydrocarbons in water bodies in seven basins of China[J]. Environmental Science, 2019, 40(5):2101-2114(in Chinese)
裴晓龙, 丁强, 杨玉珍, 等. 2013-2017年延河水质变化及其影响因素[J]. 水土保持通报, 2019, 39(2):115-117 , 123 Pei X L, Ding Q, Yang Y Z, et al. Change and influencing factors of water quality in Yanhe River from 2013 to 2017[J]. Bulletin of Soil and Water Conservation, 2019, 39(2):115-117, 123(in Chinese)
吴江涛. 延河流域水污染现状评价及管理建议[D]. 杨凌:西北农林科技大学, 2018:9-30 Wu J T. Assessment of present water pollution of Yanhe River and management suggestions[D]. Yangling:Northwest A & F University, 2018:9 -30(in Chinese)
蒋闰兰, 肖佰财, 张帆, 等. 菲胁迫对中华绒螯蟹(Eriocheir sinensis)的急性毒性效应[J]. 应用与环境生物学报, 2015, 21(1):108-114 Jiang R L, Xiao B C, Zhang F, et al. Acute toxic effects of phenanthrene on Chinese mitten crab Eriocheir sinensis[J]. Chinese Journal of Applied and Environmental Biology, 2015, 21(1):108-114(in Chinese)
肖佰财, 蒋闰兰, 李丹妮, 等. 菲对河蚬的急性毒性效应[J]. 海洋渔业, 2015, 37(1):60-67 Xiao B C, Jiang R L, Li D N, et al. Acute toxicity effects of phenanthrene on Corbicula fluminea[J]. Marine Fisheries, 2015, 37(1):60-67(in Chinese)
杨涛, 陈海刚, 蔡文贵, 等. 菲胁迫对红鳍笛鲷急、慢性毒性效应的研究[J]. 水产学报, 2011, 35(2):298-304 Yang T, Chen H G, Cai W G, et al. Acute and chronic toxicity effects of phenanthrene in seawater on crimson snapper(Lutjanus erythropterus)[J]. Journal of Fisheries of China, 2011, 35(2):298-304(in Chinese)
赵子涵. 苯并(a)芘和菲对沙蚕CYP4基因及其蛋白表达的影响[D]. 大连:大连海洋大学, 2016:6-36 Zhao Z H. The expression of CYP4 in Perinereis aibuhitensis under benzo(a)pyrene and phenanthrene exposure[D]. Dalian:Dalian Ocean University, 2016 :6-36(in Chinese)
陈玉明, 卢少勇, 朱旭, 等. 泥鳅在污染物毒性评价中的应用[J]. 生态毒理学报, 2013, 8(4):447-455 Chen Y M, Lu S Y, Zhu X, et al. Review on application of loach to assessing toxicity of pollutants[J]. Asian Journal of Ecotoxicology, 2013, 8(4):447-455(in Chinese)
Wang J Q, Fu G H, Li W Y, et al. The effects of two free-floating plants (Eichhornia crassipes and Pistia stratiotes) on the burrow morphology and water quality characteristics of pond loach (Misgurnus anguillicaudatus) habitat[J]. Aquaculture and Fisheries, 2018, 3(1):22-29 Amano H, Uno S, Koyama J, et al. Development of specific enzyme-linked immunosorbent assays for multiple vitellogenins in marbled sole, Pleuronectes yokohamae[J]. General and Comparative Endocrinology, 2019, 281:67-72 王天玉, 曹谨玲, 陈剑杰, 等. 氟对斑马鱼生殖毒性研究[J]. 环境科学学报, 2020, 40(6):2262-2270 Wang T Y, Cao J L, Chen J J, et al. Study on reproductive toxicity by fluoride in zebrafish (Danio rerio)[J]. Acta Scientiae Circumstantiae, 2020, 40(6):2262-2270(in Chinese)
王亚梅. 斑马鱼卵黄蛋白原受体基因的鉴定、表达和功能分析[D]. 兰州:西北师范大学, 2020:40-76 Wang Y M. Identification, expression and functional analysis of zebrafish vitellogenin receptor gene[D]. Lanzhou:Northwest Normal University, 2020:40 -76(in Chinese)
马境. 西伯利亚鲟卵黄发生过程中的生理调节机制与中华鲟EST文库的生物信息学分析[D]. 上海:华东师范大学, 2011:40-68 Ma J. The physiological regulation of Acipenser baerii vitellogenesis and annotation and analysis of Acipenser sinensis ESTs[D]. Shanghai:East China Normal University, 2011:40 -68(in Chinese)
詹翠琼, 黄絮洁, 方展强, 等. 多氯联苯对孔雀鱼卵黄蛋白原的诱导及检测[J]. 生态毒理学报, 2007, 2(3):333-338 Zhan C Q, Huang X J, Fang Z Q, et al. Induction and determination of vitellogenin in male guppies (Poecilia reticulata)after exposed to polychlorinated biphenyls[J]. Asian Journal of Ecotoxicology, 2007, 2(3):333-338(in Chinese)
岳南南, 王汨, 闫玉莲, 等. 饲料中不同浓度菲对中华倒刺鲃几种血液学指标和肝胰脏的影响[J]. 水生生物学报, 2014, 38(2):304-311 Yue N N, Wang M, Yan Y L, et al. Effects of dietary phe exposure on the haematological indexes and hepatopancreas of Spinibarbus sinensis[J]. Acta Hydrobiologica Sinica, 2014, 38(2):304-311(in Chinese)
赵荧, 李媛, 陈永柏. 双酚A和酞酸酯对鱼类内分泌干扰效应及繁殖毒性研究[J]. 水生态学杂志, 2017, 38(6):1-10 Zhao Y, Li Y, Chen Y B. Research progress of endocrine-disrupting effects and reproductive toxicity of bisphenol A and phthalic acid esters on fish[J]. Journal of Hydroecology, 2017, 38(6):1-10(in Chinese)
宫向红, 徐英江, 任利华, 等. 辛基酚对鲤的雌激素效应[J]. 水产学报, 2010, 34(3):410-414 Gong X H, Xu Y J, Ren L H, et al. Estrogenic effects of octylphenol on carp (Cyprinus carpio)[J]. Journal of Fisheries of China, 2010, 34(3):410-414(in Chinese)
Li X Q, Liu X F, Jia Z Z, et al. Screening of estrogenic endocrine-disrupting chemicals in meat products based on the detection of vitellogenin by enzyme-linked immunosorbent assay[J]. Chemosphere, 2021, 263:128251 黄晔, 任华, 孙竹筠, 等. 壬基酚和双酚A对雄性斑马鱼(Danio rerio)卵黄蛋白原mRNA的诱导效应[J]. 生态毒理学报, 2008, 3(3):274-279 Huang Y, Ren H, Sun Z Y, et al. Vitellogenin mRNA expression in male zebrafish (Danio rerio) induced by nonylphenol and bisphenol-A[J]. Asian Journal of Ecotoxicology, 2008, 3(3):274-279(in Chinese)
付娟娟, 郭勇勇, 韩建, 等. 苯并芘和邻苯二甲酸二(2-乙基己基)酯复合暴露对稀有鮈鲫的内分泌干扰效应研究[J]. 生态毒理学报, 2019, 14(6):93-103 Fu J J, Guo Y Y, Han J, et al. Endocrine disruption effects of benzo(a) pyrene and di-2-ethylhexyl phthalate on Chinese rare minnow (Gobiocypris rarus)[J]. Asian Journal of Ecotoxicology, 2019, 14(6):93-103(in Chinese)
Sun L B, Zuo Z H, Chen M, et al. Reproductive and transgenerational toxicities of phenanthrene on female marine medaka (Oryzias melastigma)[J]. Aquatic Toxicology, 2015, 162:109-116 陈亚文, 杨洋, 唐天乐, 等. 双酚AF对雄性斑马鱼卵黄蛋白原水平与芳香化酶基因表达的影响[J]. 生态毒理学报, 2015, 10(6):320-327 Chen Y W, Yang Y, Tang T L, et al. Effects of bisphenol AF on level of vitellogenin and expressions of cytochrome P450 aromatase (cyp 19a and b) in male zebrafish[J]. Asian Journal of Ecotoxicology, 2015, 10(6):320-327(in Chinese)
陈述. BPA和EE2调控稀有鮈鲫vtg基因表达的分子机制研究[D]. 杨凌:西北农林科技大学, 2016:30-89 Chen S. Study on the molecular mechanism of the expression of vtg gene exposed by BPA and EE2 in rare minnow Gobiocypris rarus[D]. Yangling:Northwest A & F University, 2016 :30-89(in Chinese)
杨倩, 杨先海, 刘济宁, 等. 双酚A替代物对雄性斑马鱼性激素及卵黄蛋白原水平的影响[J]. 南京工业大学学报(自然科学版), 2018, 40(5):6-13 Yang Q, Yang X H, Liu J N, et al. Effects of bisphenol A and its substitutes on levels of steroid hormone and vitellogenin in adult male zebrafish[J]. Journal of Nanjing Tech University (Natural Science Edition), 2018, 40(5):6-13(in Chinese) 程艳, 崔媛, 何平, 等. 全氟辛烷磺酸(PFOS)对斑马鱼血浆和组织匀浆中卵黄蛋白原含量的影响[J]. 生态毒理学报, 2012, 7(1):65-70 Cheng Y, Cui Y, He P, et al. Effect of perfluorooctane sulfonate (PFOS) on vitellogenin levels in blood plasma and tissue homogenate of zebrafish (Brachydanio rerio)[J]. Asian Journal of Ecotoxicology, 2012, 7(1):65-70(in Chinese)
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