微波消解-原子荧光法测定染毒小鼠体内汞的分布
Study on Hg distribution in different tissues of rats by hydride generation atomic fluorescence spectroscopy with microwave digestion
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摘要: 小鼠经腹腔注射氯化汞(HgCl2)染毒后,采用微波消解-原子荧光法研究汞在小鼠全血、脑、睾丸、脾、肝脏以及肾脏的分布情况.结果表明,方法的检出限为0.002 μg·L-1,加标回收率为92.12%—108.46%.该方法操作简便,灵敏度高,检出限低,线性范围宽,可作为生物体内汞含量的测定方法.汞在染毒小鼠血和各脏器中含量随染汞剂量的增加而明显增加,与对照组相比差异有统计学意义(P<0.05);小鼠体内总汞绝大部分蓄积在肾脏内,其次是肝脏,然后是脾、睾丸、脑;全血的总汞含量与肾脏、肝脏、脾、睾丸、脑的总汞含量有相关性.Abstract: The concentrations of mercury in brain, testis, spleen, liver, kidney and blood were measured by AFS with microwave digestion after rats were injected intraperitoneally with different doses of mercury chloride. The results showed mercury concentrations in brain, testis, spleen, liver and kidney were significantly correlated to administrated dose and statistically higher than those in the control group (PP-1, and the recovery tested by adding standards ranged from 92.1% to 108.5%.The method was simple and high sensitivity, which provided a basis for study of concentrations of mercury in different tissues of biologies.
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Key words:
- mercury /
- microwave digestion /
- atomic fluorescence spectrometry /
- relevance
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