2,3,7,8-TCDD代谢产物的液-质联用分析

王春艳, 唐志, 宋凤瑞, 蔡宗苇. 2,3,7,8-TCDD代谢产物的液-质联用分析[J]. 环境化学, 2013, 32(7): 1353-1357. doi: 10.7524/j.issn.0254-6108.2013.07.032
引用本文: 王春艳, 唐志, 宋凤瑞, 蔡宗苇. 2,3,7,8-TCDD代谢产物的液-质联用分析[J]. 环境化学, 2013, 32(7): 1353-1357. doi: 10.7524/j.issn.0254-6108.2013.07.032
WANG Chunyan, TANG Zhi, SONG Fengrui, CAI Zongwei. Analysis of in vitro metabolites of 2,3,7,8-TCDD in rat liver microsomes by LC-ESI-MS/MS[J]. Environmental Chemistry, 2013, 32(7): 1353-1357. doi: 10.7524/j.issn.0254-6108.2013.07.032
Citation: WANG Chunyan, TANG Zhi, SONG Fengrui, CAI Zongwei. Analysis of in vitro metabolites of 2,3,7,8-TCDD in rat liver microsomes by LC-ESI-MS/MS[J]. Environmental Chemistry, 2013, 32(7): 1353-1357. doi: 10.7524/j.issn.0254-6108.2013.07.032

2,3,7,8-TCDD代谢产物的液-质联用分析

  • 基金项目:

    香港浸会大学研究资助(FRG2/10-11/029)和香港研究资助委员会的普通资助(HKBU200310).

Analysis of in vitro metabolites of 2,3,7,8-TCDD in rat liver microsomes by LC-ESI-MS/MS

  • 摘要: 采用肝微粒体体外温孵法,对2,3,7,8-TCDD在大鼠肝微粒体中进行体外代谢研究.利用实验中所建立的液相色谱串联质谱方法对其代谢产物进行直接分析,共鉴别出两类代谢产物,分别为2个单羟基化代谢产物(2-OH-1,3,7,8-TCDD和1-OH-2,3,7,8-TCDD)和双羟基化脱氯的代谢产物.
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  • [1] Poland A,Knutson J C. 2,3,7,8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: Examination of the mechanism of toxicity [J]. Annu Rev Pharmacol Toxicol,1982,22: 517-524
    [2] Pirkle J L,Wolfe W H,Patterson D G,et al. Estimates of the half-life of 2,3,7,8-tetrachloro-p-dioxin in Vietnam veterans of Operation Ranch Hand [J]. J Toxicol Environ Health,1989,27: 165-171
    [3] Sorg O,Zennegg M,Schmid P,et al. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) poisoning in Victor Yushchenko: Identification and measurement of TCDD metabolites[J]. Lancet,2009,374(9696): 1179-1185
    [4] Hu K,Bunce N J. Metabolism of polychlorinated dibenzo-p-dioxins and related dioxin-like compounds [J]. J Toxicol Environ Health B Crit Rev,1999,2(2): 183-210
    [5] Van den Berg M,De Jongh J,Poiger H,et al. The toxicokinetics and metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) and their relevance for toxicity [J]. Crit Rev Toxicol,1994,24(1): 1-74
    [6] Weber H,Poiger H,Schlatter C. Acute oral toxicity of TCDD-metabolites in male guinea pigs [J]. Toxicol Lett,1982,14(1/2): 117-122
    [7] Mason G,Safe S. Synthesis,biologic and toxic effects of the major 2,3,7,8-tetrachlorodibenzo-p-dioxin metabolites in the rat [J]. Toxicology,1986,41(2): 153-159
    [8] Birnbaum L S. The role of structure in the disposition of halogenated aromatic xenobiotics [J]. Environ Health Perspect,1985,61: 11-20
    [9] Sawahata T,Olson J R,Neal R A. Identification of metabolites of 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes [J]. Biochem Biophys Res Commun,1982,105(1): 341-346
    [10] Inouye K,Shinkyo R,Takita T,et al. Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by human cytochrome P450-dependent monooxygenase systems [J]. J Agric Food Chem,2002,50(19): 5496-5502
    [11] Poiger H,Buser H R,Weber H,et al. Structure elucidation of mammalian TCDD-metabolites [J]. Experientia,1982,38(4): 484-486
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  • 收稿日期:  2013-01-02

2,3,7,8-TCDD代谢产物的液-质联用分析

  • 1.  香港浸会大学化学系,香港特别行政区;
  • 2.  中国科学院长春应用化学研究所,长春,130022
基金项目:  香港浸会大学研究资助(FRG2/10-11/029)和香港研究资助委员会的普通资助(HKBU200310).

摘要: 采用肝微粒体体外温孵法,对2,3,7,8-TCDD在大鼠肝微粒体中进行体外代谢研究.利用实验中所建立的液相色谱串联质谱方法对其代谢产物进行直接分析,共鉴别出两类代谢产物,分别为2个单羟基化代谢产物(2-OH-1,3,7,8-TCDD和1-OH-2,3,7,8-TCDD)和双羟基化脱氯的代谢产物.

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