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李玄, 王锐, 尹大强. 饮用水汞暴露对小鼠免疫系统的毒性[J]. 环境化学, 2014, 33(9): 1427-1432. doi: 10.7524/j.issn.0254-6108.2014.09.012
引用本文: 李玄, 王锐, 尹大强. 饮用水汞暴露对小鼠免疫系统的毒性[J]. 环境化学, 2014, 33(9): 1427-1432. doi: 10.7524/j.issn.0254-6108.2014.09.012
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LI Xuan, WANG Rui, YIN Daqiang. Immunotoxic effects of mercury exposure via drinking water[J]. Environmental Chemistry, 2014, 33(9): 1427-1432. doi: 10.7524/j.issn.0254-6108.2014.09.012
Citation: LI Xuan, WANG Rui, YIN Daqiang. Immunotoxic effects of mercury exposure via drinking water[J]. Environmental Chemistry, 2014, 33(9): 1427-1432. doi: 10.7524/j.issn.0254-6108.2014.09.012
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饮用水汞暴露对小鼠免疫系统的毒性

  • 1.

    同济大学环境科学与工程学院, 上海, 200092

  • 基金项目:

    高等学校博士点基金（20130072120031）；国家自然科学基金（51278353）；博士点基金（20110072110021）资助.

Immunotoxic effects of mercury exposure via drinking water

  • 1.

    College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

  • Fund Project:

  • 摘要: 通过饮用水对小鼠进行氯化汞和氯化甲基汞暴露，连续暴露14 d后，采集小鼠血样、脾脏与胸腺.分别采用MTT法、LDH释放法和ELISA等方法评价了汞暴露对小鼠胸腺、脾脏的淋巴细胞体外增殖系数、自然杀伤细胞杀伤系数和血清中细胞因子（IL-2和TNF-α）分泌量的影响.实验结果显示：（1）汞暴露抑制了淋巴细胞的增殖性能和自然杀伤细胞杀伤活性，并具有浓度依赖性，且MeHg的抑制作用明显高于Hg2+；（2）MeHg和Hg2+暴露同样对IL-2的分泌具有抑制作用但不具有浓度依赖性；（3）汞暴露对TNF-α分泌的影响依汞的形态和浓度不同而不同，如MeHg对其具有明显抑制作用，Hg2+在低浓度时对TNF-α的分泌具有一定的促进作用，而高浓度时为抑制作用.
    Abstract: In present study, mice were continuously exposed to MeHgCl or HgCl2 via drinking water for 14 days. At the end of exposure, blood were sampled from the eyes of the mice. After that, thymuses and spleens were removed. MTT, LDH release and ELISA assays were used to assess the lymphocyte proliferation, the activity of NK cells and the production of serum IL-2 and TNF-α. The results showed that both MeHg and Hg2+ suppressed the lymphocyte proliferation and the activity of NK cells in a dose dependent manner, and the suppression of MeHg was higher than that of Hg2+. However, there was no dose-dependent relationship between the exposure level of Hg and the inhibition effects on the serum IL-2 production by either MeHg or Hg2+. What's more, the effect of Hg on serum TNF-α production varied with Hg species and Hg exposure levels, i.e., MeHg significantly suppressed serum TNF-α production while Hg2+ increased it at low and middle exposure levels but decreased it at high exposure levels.
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  • 收稿日期:  2013-12-18

饮用水汞暴露对小鼠免疫系统的毒性

  • 1. 同济大学环境科学与工程学院, 上海, 200092
  • 收稿日期:  2013-12-18
基金项目:

高等学校博士点基金（20130072120031）；国家自然科学基金（51278353）；博士点基金（20110072110021）资助.

摘要: 通过饮用水对小鼠进行氯化汞和氯化甲基汞暴露，连续暴露14 d后，采集小鼠血样、脾脏与胸腺.分别采用MTT法、LDH释放法和ELISA等方法评价了汞暴露对小鼠胸腺、脾脏的淋巴细胞体外增殖系数、自然杀伤细胞杀伤系数和血清中细胞因子（IL-2和TNF-α）分泌量的影响.实验结果显示：（1）汞暴露抑制了淋巴细胞的增殖性能和自然杀伤细胞杀伤活性，并具有浓度依赖性，且MeHg的抑制作用明显高于Hg2+；（2）MeHg和Hg2+暴露同样对IL-2的分泌具有抑制作用但不具有浓度依赖性；（3）汞暴露对TNF-α分泌的影响依汞的形态和浓度不同而不同，如MeHg对其具有明显抑制作用，Hg2+在低浓度时对TNF-α的分泌具有一定的促进作用，而高浓度时为抑制作用.

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