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荧光光谱法研究草甘膦与腐殖酸的相互作用

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孙梅香, 刘文, 高嘉苓, 吴曼, 张业中, 戴捷. 荧光光谱法研究草甘膦与腐殖酸的相互作用[J]. 环境化学, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
引用本文: 孙梅香, 刘文, 高嘉苓, 吴曼, 张业中, 戴捷. 荧光光谱法研究草甘膦与腐殖酸的相互作用[J]. 环境化学, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
shu
SUN Meixiang, LIU Wen, GAO Jialing, WU Man, ZHANG Yezhong, DAI Jie. Study on the interaction between glyphosate and humic acid using fluorescence spectrometry and parallel factor analysis method[J]. Environmental Chemistry, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
Citation: SUN Meixiang, LIU Wen, GAO Jialing, WU Man, ZHANG Yezhong, DAI Jie. Study on the interaction between glyphosate and humic acid using fluorescence spectrometry and parallel factor analysis method[J]. Environmental Chemistry, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
shu

荧光光谱法研究草甘膦与腐殖酸的相互作用

  • 1.

    长江大学化学与环境工程学院, 荆州, 434023

  • 基金项目:

    国家自然科学基金 (21173026)

    湖北省自然科学基金重点项目(2013CFA107)资助.

Study on the interaction between glyphosate and humic acid using fluorescence spectrometry and parallel factor analysis method

  • 1.

    College of Chemical and Environmental Engineering, Yangtze University, Jingzhou, 434023, China

  • Fund Project:

  • 摘要: 应用荧光光谱法研究了草甘膦(PMG)与腐殖酸(HA)的相互作用.荧光猝灭实验表明,PMG对HA的荧光产生静态猝灭;通过对不同温度下反应热力学参数的计算,发现二者相互间作用力主要是氢键和范德华力.受到PMG作用的影响,HA的同步荧光光谱出现了蓝移现象,初步说明HA在作用后共轭度降低.采用平行因子分析技术对加入PMG前后的HA三维荧光光谱进行解析,发现加入PMG后HA荧光组分不仅出现在区域Ⅴ(难降解有机物荧光区)中,而且有部分出现在了区域Ⅲ(可降解有机物荧光区)中,总荧光强度明显减弱,而且组分C3、C4有明显的蓝移现象.三维荧光光谱分析验证了荧光猝灭及同步荧光的研究结果,并进一步表明,PMG与HA的相互作用使HA共轭度降低,并且可能有可降解物质生成.
    Abstract: The interaction between glyphosate (PMG) and humic acid (HA) were investigated by fluorescence spectroscopy method. The fluorescence quenching experiment indicated that fluorescence intensity of HA was decreased by PMG owing to a static quenching process. The binding constants and thermodynamics parameters of PMG with HA were obtained at different temperatures,and the results showed that hydrogen bonds and Van der Waals forces played a main role in the binding of PMG with HA. The blue shift of synchronous fluorescence spectra showed that the binding with PMG reduced the conjugation of HA. In addition, parallel factor (PARAFAC) analysis was employed to analyze the three dimensional fluorescence spectra of the HA. Firstly, the results displayed that all the fluorescent components of HA were in the region Ⅴ which is the refractory organics region. After adding PMG into HA, some peaks of HA were in the region Ⅲ which is the biodegradable organics region. Secondly, the total fluorescence intensity of HA was decreased. Thirdly, the fluorescent component C3 and C4 of HA disdayed blue shift. It not only confirmed the results of the fluorescence quenching spectra and synchronous fluorescence spectroscopy, but also demonstrated that the interaction between HA and PMG reduced the conjugation of HA and may generate some biodegradable substances.
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  • 收稿日期:  2014-12-24
  • 刊出日期:  2015-08-15
孙梅香, 刘文, 高嘉苓, 吴曼, 张业中, 戴捷. 荧光光谱法研究草甘膦与腐殖酸的相互作用[J]. 环境化学, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
引用本文: 孙梅香, 刘文, 高嘉苓, 吴曼, 张业中, 戴捷. 荧光光谱法研究草甘膦与腐殖酸的相互作用[J]. 环境化学, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
shu
SUN Meixiang, LIU Wen, GAO Jialing, WU Man, ZHANG Yezhong, DAI Jie. Study on the interaction between glyphosate and humic acid using fluorescence spectrometry and parallel factor analysis method[J]. Environmental Chemistry, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
Citation: SUN Meixiang, LIU Wen, GAO Jialing, WU Man, ZHANG Yezhong, DAI Jie. Study on the interaction between glyphosate and humic acid using fluorescence spectrometry and parallel factor analysis method[J]. Environmental Chemistry, 2015, 34(8): 1529-1534. doi: 10.7524/j.issn.0254-6108.2015.08.2014122401
shu

荧光光谱法研究草甘膦与腐殖酸的相互作用

  • 1. 长江大学化学与环境工程学院, 荆州, 434023
  • 收稿日期:  2014-12-24
基金项目:

国家自然科学基金 (21173026)

湖北省自然科学基金重点项目(2013CFA107)资助.

摘要: 应用荧光光谱法研究了草甘膦(PMG)与腐殖酸(HA)的相互作用.荧光猝灭实验表明,PMG对HA的荧光产生静态猝灭;通过对不同温度下反应热力学参数的计算,发现二者相互间作用力主要是氢键和范德华力.受到PMG作用的影响,HA的同步荧光光谱出现了蓝移现象,初步说明HA在作用后共轭度降低.采用平行因子分析技术对加入PMG前后的HA三维荧光光谱进行解析,发现加入PMG后HA荧光组分不仅出现在区域Ⅴ(难降解有机物荧光区)中,而且有部分出现在了区域Ⅲ(可降解有机物荧光区)中,总荧光强度明显减弱,而且组分C3、C4有明显的蓝移现象.三维荧光光谱分析验证了荧光猝灭及同步荧光的研究结果,并进一步表明,PMG与HA的相互作用使HA共轭度降低,并且可能有可降解物质生成.

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