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虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能

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宋兴良, 王爱香, 刘晓泓, 黄怡, 石莉莉. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 环境化学, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
引用本文: 宋兴良, 王爱香, 刘晓泓, 黄怡, 石莉莉. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 环境化学, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
shu
SONG Xingliang, WANG Aixiang, LIU Xiaohong, HUANG Yi, SHI Lili. Preparation of analog-molecularly imprinted microspheres and their adsorbent performance for microcystin in aqueous solution[J]. Environmental Chemistry, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
Citation: SONG Xingliang, WANG Aixiang, LIU Xiaohong, HUANG Yi, SHI Lili. Preparation of analog-molecularly imprinted microspheres and their adsorbent performance for microcystin in aqueous solution[J]. Environmental Chemistry, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
shu

虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能

  • 1.

    临沂大学化学化工学院, 临沂, 276005

  • 基金项目:

    国家自然科学基金(21275068)资助.

Preparation of analog-molecularly imprinted microspheres and their adsorbent performance for microcystin in aqueous solution

  • 1.

    School of Chemistry & Chemical Engineering, Linyi University, Linyi, 276005, China

  • Fund Project: Supported by the National Natural Science Foundation of China (21275068).

  • 摘要: 本文以L-精氨酸为模板、丙烯酰胺(AM)为单体,N, N'-亚甲基双丙烯酰胺(MBA)为交联剂以及偶氮二异丁腈(AIBN)为引发剂,采用沉淀聚合法制备了微囊藻毒素分子印迹聚合物.采用量子化学密度泛函理论(DFT)模拟计算以确定模板分子(L-精氨酸)与功能单体(AM)的印迹比例,通过扫描电镜和红外吸收光谱对分子印迹聚合物微球进行表征,并利用平衡吸附实验考察了印迹微球对微囊藻毒素的吸附性能.结果表明,本虚拟模板法制备的印迹微球对MC-LR的吸附符合Langmuir模型,线性拟合得出MIPs的线性回归方程为Ce/Q=3.026×10-4+0.125(r=0.991),由此推导出对MC-LR的最大表观吸附量为80.3 μg·g-1.实验还表明,印迹聚合物对MC-LR和MC-RR的吸附比非印迹聚合物具有更好的选择性,MIPs对MC-LR的初始吸附速率为58.8 μg·g-1·min-1,大于非印迹聚合物的初始吸附速率19.6 μg·g-1·min-1,具有选择性富集水体中MC-LR和MC-RR等微囊藻毒素的应用前景.
    Abstract: Molecularly imprinted polymers for microcystins were synthesized by precipitation polymerization method using L-arginine as the template, acrylamide(AM) as monomer, N, N'-Methylenebisacrylamide as crosslinker and azodiisobutyronitrile as initiator. The imprinting ratio of L-arginine and acrylamide (AM) was calculated based on the Density Functional Theory (DFT) method, and the resulting composites were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis. The selectivity of the MIPs for the adsorption of microcystin-LR was demonstrated by equilibrium adsorption experiments.Results showed that the adsorption isotherms of analog-molecularly imprinted microspheres to MC-LR can be described by the Langmuir adsorption model, and the linear regression equation was fitted as Ce/Q=3.026×10-4+0.125 (r=0.991), and the apparent maximum adsorption capacity for MC-LR was 80.3 μg·g-1. It was proved that the MIPs had better molecular fragment recognition ability for MC-LR and MC-RR than for of NIPs. The imprinted microspheres showed rapid adsorption dynamic process because the initial adsorption rate of MIPs and NIPs to MC-LR were 58.8, 19.6 μg·g-1·min-1, respectively. This polymers can be used for selective extraction of[arginine]-microcystins in water using solid phase extraction method in future.
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  • 收稿日期:  2015-02-13
  • 刊出日期:  2016-03-15
宋兴良, 王爱香, 刘晓泓, 黄怡, 石莉莉. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 环境化学, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
引用本文: 宋兴良, 王爱香, 刘晓泓, 黄怡, 石莉莉. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 环境化学, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
shu
SONG Xingliang, WANG Aixiang, LIU Xiaohong, HUANG Yi, SHI Lili. Preparation of analog-molecularly imprinted microspheres and their adsorbent performance for microcystin in aqueous solution[J]. Environmental Chemistry, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
Citation: SONG Xingliang, WANG Aixiang, LIU Xiaohong, HUANG Yi, SHI Lili. Preparation of analog-molecularly imprinted microspheres and their adsorbent performance for microcystin in aqueous solution[J]. Environmental Chemistry, 2016, 35(3): 451-459. doi: 10.7524/j.issn.0254-6108.2016.03.2015021301
shu

虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能

  • 1. 临沂大学化学化工学院, 临沂, 276005
  • 收稿日期:  2015-02-13
基金项目:

国家自然科学基金(21275068)资助.

摘要: 本文以L-精氨酸为模板、丙烯酰胺(AM)为单体,N, N'-亚甲基双丙烯酰胺(MBA)为交联剂以及偶氮二异丁腈(AIBN)为引发剂,采用沉淀聚合法制备了微囊藻毒素分子印迹聚合物.采用量子化学密度泛函理论(DFT)模拟计算以确定模板分子(L-精氨酸)与功能单体(AM)的印迹比例,通过扫描电镜和红外吸收光谱对分子印迹聚合物微球进行表征,并利用平衡吸附实验考察了印迹微球对微囊藻毒素的吸附性能.结果表明,本虚拟模板法制备的印迹微球对MC-LR的吸附符合Langmuir模型,线性拟合得出MIPs的线性回归方程为Ce/Q=3.026×10-4+0.125(r=0.991),由此推导出对MC-LR的最大表观吸附量为80.3 μg·g-1.实验还表明,印迹聚合物对MC-LR和MC-RR的吸附比非印迹聚合物具有更好的选择性,MIPs对MC-LR的初始吸附速率为58.8 μg·g-1·min-1,大于非印迹聚合物的初始吸附速率19.6 μg·g-1·min-1,具有选择性富集水体中MC-LR和MC-RR等微囊藻毒素的应用前景.

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