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电化学检测氯酚类污染物对MCF-7细胞的毒性

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秦洪伟, 闫彬, 尤国红, 武冬梅, 丛俏, 袁星. 电化学检测氯酚类污染物对MCF-7细胞的毒性[J]. 环境化学, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
引用本文: 秦洪伟, 闫彬, 尤国红, 武冬梅, 丛俏, 袁星. 电化学检测氯酚类污染物对MCF-7细胞的毒性[J]. 环境化学, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
shu
QIN Hongwei, YAN Bin, YOU Guohong, WU Dongmei, CONG Qiao, YUAN Xing. Electrochemical assessment of cytotoxicity of chlorophenols on MCF-7 cell[J]. Environmental Chemistry, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
Citation: QIN Hongwei, YAN Bin, YOU Guohong, WU Dongmei, CONG Qiao, YUAN Xing. Electrochemical assessment of cytotoxicity of chlorophenols on MCF-7 cell[J]. Environmental Chemistry, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
shu

电化学检测氯酚类污染物对MCF-7细胞的毒性

  • 1.

    渤海大学化学化工学院, 锦州, 121013;

  • 2.

    渤海大学工学院, 锦州, 121013;

  • 3.

    佳木斯大学药学院, 佳木斯, 154007;

  • 4.

    东北师范大学环境学院, 长春, 130117

  • 基金项目:

    国家自然科学基金（41602351，61401043，51309013）和辽宁省博士科研启动基金（201601344）资助.

Electrochemical assessment of cytotoxicity of chlorophenols on MCF-7 cell

  • 1.

    College of Chemistry and Chemical Engineering, Bohai University, Jinzhou, 121013, China;

  • 2.

    College of Engineering, Bohai University, Jinzhou, 121013, China;

  • 3.

    College of Pharmacy, Jiamusi University, Jiamusi, 154007, China;

  • 4.

    School of Environment, Northeast Normal University, Changchun, 130117, China

  • Fund Project: Supported by the National Natural Science Foundation of China (41602351,61401043,51309013) and the Doctoral Scientific Research Starting Foundation of Liaoning Province,China (201601344).

  • 摘要: 本文以石墨烯修饰电极（Gr-GCE）为工作电极，以人乳腺癌MCF-7细胞为模型细胞，运用线性扫描伏安法研究了MCF-7细胞的裂解液电化学行为，确定其响应来源为黄嘌呤和鸟嘌呤的电化学氧化. 跟踪描述了细胞生长曲线，研究了五氯酚（PCP）、2，4，6-三氯酚（TCP）和2，4-二氯酚（DCP）对MCF-7细胞的毒性，计算得到半数抑制效应浓度（IC50）值分别为77.62 、174.08、449.78 μmol·L-1，并与四甲基偶氮唑盐（MTT）比色法进行比较，结果表明氯酚类污染物对MCF-7细胞活性有明显抑制作用，两种方法测得细胞毒性顺序均为：PCP > TCP > DCP，且细胞电化学法更为灵敏.
    Abstract: In this paper,electrochemical response of lysed human cervical carcinoma (MCF-7) cells on graphene modified electrode was studied,using linear sweep voltammetry. The current was confinmed to result from the oxidation of guanine and xanthine in the cytoplasm of MCF-7 cells. Then the growth of MCF-7 cells was followed by the electrochemical response. The cytotoxicity of pentachlorophenol (PCP),2,4,6-trichlorophenol (TCP) and 2,4-dichlorophenol (DCP) on MCF-7 cells were assessed,and the 50% inhibitory concentration were calculated,which were 77.62 μmol·L-1,174.08 μmol·L-1 and 449.78 μmol·L-1,respectively. Furthermore,the results were compared with those obtained from the methyl tetrazolium (MTT) assay. The results implied that the chlorophenols significantly inhibited the viability of MCF-7 cell. The order of toxicity obtained from the electrochemical detection method and the MTT assay were both PCP > TCP > DCP,and the cell electrochemical detection method was more sensitive.
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  • 收稿日期:  2016-10-28
  • 刊出日期:  2017-07-15
秦洪伟, 闫彬, 尤国红, 武冬梅, 丛俏, 袁星. 电化学检测氯酚类污染物对MCF-7细胞的毒性[J]. 环境化学, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
引用本文: 秦洪伟, 闫彬, 尤国红, 武冬梅, 丛俏, 袁星. 电化学检测氯酚类污染物对MCF-7细胞的毒性[J]. 环境化学, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
shu
QIN Hongwei, YAN Bin, YOU Guohong, WU Dongmei, CONG Qiao, YUAN Xing. Electrochemical assessment of cytotoxicity of chlorophenols on MCF-7 cell[J]. Environmental Chemistry, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
Citation: QIN Hongwei, YAN Bin, YOU Guohong, WU Dongmei, CONG Qiao, YUAN Xing. Electrochemical assessment of cytotoxicity of chlorophenols on MCF-7 cell[J]. Environmental Chemistry, 2017, 36(7): 1433-1439. doi: 10.7524/j.issn.0254-6108.2017.07.2016102803
shu

电化学检测氯酚类污染物对MCF-7细胞的毒性

  • 1.  渤海大学化学化工学院, 锦州, 121013;
  • 2.  渤海大学工学院, 锦州, 121013;
  • 3.  佳木斯大学药学院, 佳木斯, 154007;
  • 4.  东北师范大学环境学院, 长春, 130117
  • 收稿日期:  2016-10-28
基金项目:

国家自然科学基金（41602351，61401043，51309013）和辽宁省博士科研启动基金（201601344）资助.

摘要: 本文以石墨烯修饰电极（Gr-GCE）为工作电极，以人乳腺癌MCF-7细胞为模型细胞，运用线性扫描伏安法研究了MCF-7细胞的裂解液电化学行为，确定其响应来源为黄嘌呤和鸟嘌呤的电化学氧化. 跟踪描述了细胞生长曲线，研究了五氯酚（PCP）、2，4，6-三氯酚（TCP）和2，4-二氯酚（DCP）对MCF-7细胞的毒性，计算得到半数抑制效应浓度（IC50）值分别为77.62 、174.08、449.78 μmol·L-1，并与四甲基偶氮唑盐（MTT）比色法进行比较，结果表明氯酚类污染物对MCF-7细胞活性有明显抑制作用，两种方法测得细胞毒性顺序均为：PCP > TCP > DCP，且细胞电化学法更为灵敏.

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