微藻对无机汞和甲基汞的吸附和吸收特性
Adsorption and absorption characteristics of inorganic mercury and methylmercury by microalgae
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摘要: 本文选取蛋白核小球藻和斜生栅藻两种微藻作为研究对象，将其接种于含有低浓度无机汞（0.1—2.0 μg·L-1）和甲基汞（5.0—100 ng·L-1）的培养基中，考察两种藻的耐受性及微藻对无机汞及甲基汞的吸附和吸收特性. 结果表明，在实验浓度范围内，0.1 μg·L-1的无机汞和5.0 ng·L-1的甲基汞即可抑制蛋白核小球藻和斜生栅藻的生长，抑制作用随汞浓度的升高而增强.超过60%的无机汞和70%的甲基汞在24 h内通过吸附和吸收快速转移到了微藻，只有少量汞化合物残留于培养基中，168 h后，两种藻对无机汞和甲基汞的最高去除率分别为99.75%和99.82%.单个微藻细胞对于无机汞和甲基汞的吸附和吸收均在24 h达到最大值，随培养时间的延长，细胞增殖产生的稀释效应导致单细胞吸附量和吸收量逐渐降低.实验中观察到了无机汞和甲基汞在微藻细胞表面吸附及内部吸收的转换.Abstract: In this study,two species of microalgae,Chlorella pyrenoidosa and Scenedesmus obliquus,were inoculated in culture media containing low concentrations of inorganic mercury (Hg2+) (0.1—2.0 μg·L-1) and methylmercury (MeHg) (5.0—100 ng·L-1) to investigate the inhibition effects and adsorption/absorption characteristics of Hg2+ and MeHg. The results showed that 0.1 μg·L-1 Hg2+ and 5.0 ng·L-1 MeHg inhibited the growth of microalgae,and the inhibitory effect was enhanced with the increase of Hg concentrations. Transport of Hg2+ and MeHg from the culture media to microalgae was a relatively fast process,with more than 60% Hg2+ and 70% MeHg being transported to microalgae within 24 hours,while only a small amount of mercury compounds remained in the medium. After 168 h,the highest removal rates of Hg2+ and MeHg by micoralgae were 99.75% and 99.82%,respectively. The amounts of adsorption and absorption of both Hg species by single microalgae cells reached the maxima at the 24 h time point,and then dropped because of the growth dilution effect resulted from the cell proliferation of microalgae. Conversion of Hg between adsorption and absorption forms was indicated by the opposite trends observed in the adsorption/absorption curves.
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Key words:
- microalgae /
- inorganic mercury /
- methylmercury /
- adsorption /
- absorption
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