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纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应

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章哲超, 胡佶, 刘淑霞, 张偲, 刘小涯, 叶陈军, 潘建明. 纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应[J]. 环境化学, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
引用本文: 章哲超, 胡佶, 刘淑霞, 张偲, 刘小涯, 叶陈军, 潘建明. 纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应[J]. 环境化学, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
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ZHANG Zhechao, HU Ji, LIU Shuxia, ZHANG Cai, LIU Xiaoya, YE Chenjun, PAN Jianming. Effect of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatum[J]. Environmental Chemistry, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
Citation: ZHANG Zhechao, HU Ji, LIU Shuxia, ZHANG Cai, LIU Xiaoya, YE Chenjun, PAN Jianming. Effect of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatum[J]. Environmental Chemistry, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
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纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应

  • 1.

    国家海洋局第二海洋研究所, 国家海洋局海洋生态系统与生物地球化学重点实验室, 杭州, 310012;

  • 2.

    青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 青岛, 266071

  • 基金项目:

    国家自然科学基金青年基金（21307019），海洋公益性行业科研专项（201505034），国家重点研发计划重点专项（2016YFC1402405）和青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题（2016LMFS-B19）资助.

Effect of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatum

  • 1.

    Second Institute of Oceanography, Hangzhou, 310012, China;

  • 2.

    Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

  • Fund Project: Supported by the National Natural Science Foundation of China (21307019), the Public Science and Technology Research Funds Projects of Ocean(201505034), the National Key Research and Development Program(2016YFC1402405) and Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (2016LMFS-B19).

摘要

  • 摘要: 随着纳米材料在日常生产生活中的广泛应用，部分纳米颗粒物不可避免地会通过废弃物排放等途径进入海洋.当纳米颗粒物与海洋中的污染物（如与重金属）共存时，因其独特的物化特性往往会成为污染物的良好载体并在生物体内累积，从而增加已有污染物和生物的相互作用，对海洋环境构成潜在的生态风险.已有的研究更多关注单一纳米材料的生态毒性效应，有关纳米颗粒物与污染物的复合生物效应的研究较少.因此，本文研究了已广泛应用的纳米SiO2与常见的重金属污染物Hg2+对东海常见海洋微藻-中肋骨条藻（Skeletonema costatum）的联合毒性效应.结果表明，Hg2+会抑制中肋骨条藻的生长，24 h-EC50、48 h-EC50和72 h-EC50值分别为56.3 μg·L-1、58.6 μg·L-1和36.8 μg·L-1；低浓度的纳米SiO2（1 mg·L-1和5 mg·L-1）未对中肋骨条藻的生长产生抑制作用，而较高浓度的纳米SiO2（≥ 10 mg·L-1）会显著（P-1纳米SiO2会增强Hg2+对中肋骨条藻的生长抑制作用，Hg2+的24 h-EC50和48 h-EC50分别下降至41 μg·L-1和43 μg·L-1，虽然1 mg·L-1纳米SiO2本身没有对中肋骨条藻产生生长抑制作用，但是能够明显增强Hg2+对中肋骨条藻的毒性.纳米SiO2对Hg2+有着较强的吸附能力，在60 min时，100 mg·L-1纳米SiO2对100 μg·L-1的Hg2+的吸附率为90.08%，最大吸附量为5.92 mg·g-1.吸附了Hg2+的纳米SiO2在中肋骨条藻内的累积可能是造成这种协同毒性的主要原因.
    Abstract: With the wide application of nanomaterials in our life, a certain amount of nanoparticles(NPs) will be released into the ocean inevitably through waste discharge. When these released NPs interact with contaminants such as heavy metals in the ocean, they can act as efficient carriers of contaminants and accmulate in organisms to increase interaction between contaminants and organisms, which poses potential ecological risks to marine environment. Most previous reports focused on eco-toxic effects of single nanomaterials. There are few reports about the joint effects of nanometer particles existed with other pollutants. Therefore, effects of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatuma were studied in this work. Results showed that Hg2+ inhibited the growth of Skeletonema costatum. The EC50 of Hg2+ after 24 h, 48 h, and 72 h was 56.3 μg·L-1,58.6 μg·L-1 and 36.8 μg·L-1, respectively. Nano-SiO2 of low concentration (1 mg·L-1 and 5 mg·L-1) did not inhibit the growth of Skeletonema costatum. However, nano-SiO2 of high concentration (10 mg·L-1 or higher) significantly(PSkeletonema costatum and promoted activity of superoxide dismutase (SOD) to affect antioxidant system of the marine microalgae. The inhibition of Hg2+ to the growth of Skeletonema costatum increased with the addition of (mg·L-1) nano-SiO2 which lowed 24 h-EC50 and 48 h-EC50 of Hg2+to 41 μg·L-1 and 43 μg·L-1, respectively. Nano-SiO2 of 1 mg·L-1 did not inhibit the growth of algae but enhanced the toxicity of Hg2+ to Skeletonema costatum. Nano- SiO2 had strong adsorption capacity for Hg2+.The adsorption rate of 100 μg·L-1 Hg2+ to 100 mg·L-1 nano-SiO2 in 60 min was 90.08%, with the maximum adsorption capacity of 5.92 mg·g-1.Accumulation of nano-SiO2 adsorbed with Hg2+inside the micro algae might be the main reason for the joint effects.
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出版历程
  • 收稿日期:  2017-08-15
  • 刊出日期:  2018-04-15
章哲超, 胡佶, 刘淑霞, 张偲, 刘小涯, 叶陈军, 潘建明. 纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应[J]. 环境化学, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
引用本文: 章哲超, 胡佶, 刘淑霞, 张偲, 刘小涯, 叶陈军, 潘建明. 纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应[J]. 环境化学, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
shu
ZHANG Zhechao, HU Ji, LIU Shuxia, ZHANG Cai, LIU Xiaoya, YE Chenjun, PAN Jianming. Effect of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatum[J]. Environmental Chemistry, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
Citation: ZHANG Zhechao, HU Ji, LIU Shuxia, ZHANG Cai, LIU Xiaoya, YE Chenjun, PAN Jianming. Effect of nano-SiO2 on the toxicity of Hg2+ to Skeletonema costatum[J]. Environmental Chemistry, 2018, 37(4): 661-669. doi: 10.7524/j.issn.0254-6108.2017081506
shu

纳米二氧化硅与汞(Hg2+)对中肋骨条藻(Skeletonema costatum)的联合毒性效应

  • 1.  国家海洋局第二海洋研究所, 国家海洋局海洋生态系统与生物地球化学重点实验室, 杭州, 310012;
  • 2.  青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 青岛, 266071
  • 收稿日期:  2017-08-15
基金项目:

国家自然科学基金青年基金（21307019），海洋公益性行业科研专项（201505034），国家重点研发计划重点专项（2016YFC1402405）和青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题（2016LMFS-B19）资助.

摘要: 随着纳米材料在日常生产生活中的广泛应用，部分纳米颗粒物不可避免地会通过废弃物排放等途径进入海洋.当纳米颗粒物与海洋中的污染物（如与重金属）共存时，因其独特的物化特性往往会成为污染物的良好载体并在生物体内累积，从而增加已有污染物和生物的相互作用，对海洋环境构成潜在的生态风险.已有的研究更多关注单一纳米材料的生态毒性效应，有关纳米颗粒物与污染物的复合生物效应的研究较少.因此，本文研究了已广泛应用的纳米SiO2与常见的重金属污染物Hg2+对东海常见海洋微藻-中肋骨条藻（Skeletonema costatum）的联合毒性效应.结果表明，Hg2+会抑制中肋骨条藻的生长，24 h-EC50、48 h-EC50和72 h-EC50值分别为56.3 μg·L-1、58.6 μg·L-1和36.8 μg·L-1；低浓度的纳米SiO2（1 mg·L-1和5 mg·L-1）未对中肋骨条藻的生长产生抑制作用，而较高浓度的纳米SiO2（≥ 10 mg·L-1）会显著（P-1纳米SiO2会增强Hg2+对中肋骨条藻的生长抑制作用，Hg2+的24 h-EC50和48 h-EC50分别下降至41 μg·L-1和43 μg·L-1，虽然1 mg·L-1纳米SiO2本身没有对中肋骨条藻产生生长抑制作用，但是能够明显增强Hg2+对中肋骨条藻的毒性.纳米SiO2对Hg2+有着较强的吸附能力，在60 min时，100 mg·L-1纳米SiO2对100 μg·L-1的Hg2+的吸附率为90.08%，最大吸附量为5.92 mg·g-1.吸附了Hg2+的纳米SiO2在中肋骨条藻内的累积可能是造成这种协同毒性的主要原因.

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