高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物

王大鹏, 张娴, 颜昌宙. 高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J]. 环境化学, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
引用本文: 王大鹏, 张娴, 颜昌宙. 高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J]. 环境化学, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
WANG Dapeng, ZHANG Xian, YAN Changzhou. Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
Citation: WANG Dapeng, ZHANG Xian, YAN Changzhou. Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804

高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物

  • 基金项目:

    国家国际科技合作专项(2011DFB91710)资助.

Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry

  • Fund Project: Supported by the International Science & Technology Cooperation Program of China (2011DFB91710).
  • 摘要: 本文建立了一种检测污水及污泥中4种磺胺类抗生素及其相应的乙酰化代谢物的分析方法,包括磺胺嘧啶(SD)、磺胺甲基嘧啶(SM1)、磺胺二甲基嘧啶(SM2)、磺胺甲恶唑(SMZ)以及它们相对应的乙酰化代谢物.本文采用了固相萃取(SPE)的方法作为前处理,并优化了固相萃取条件.计算了样品中复杂的基质效应,并使用内标法来抵消基质效应.该方法在污水中检测限(LOD)和定量限(LOQ)分别为0.12-2.06 ng·L-1和0.43-5.12 ng·L-1,在污泥中分别为0.28-2.31 ng·g-1和0.88-6.77 ng·g-1,在污水和污泥中的回收率为分别为70.8%-130.6%,88.0%-129.2%.本方法成功应用于检测污水处理厂中的污水及污泥中的目标化合物.
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  • [1] ALVARINO T, NASTOLD P, SUAREZ S, et al. Role of biotransformation, sorption and mineralization of (14)C-labelled sulfamethoxazole under different redox conditions[J]. Science of the Total Environment, 2016, 542:706-715.
    [2] WANG N, GUO X Y, XU J, et al. Sorption and transport of five sulfonamide antibiotics in agricultural soil and soil-manure systems[J]. Journal of Environmental Science and Health Part B:Pesticides, Food Contaminants, and Agricultural Wastes, 2015, 50(1):23-33.
    [3] TASHO R P, CHO J Y. Veterinary antibiotics in animal waste, its distribution in soil and uptake by plants:A review[J]. Science of the Total Environment, 2016, 563:366-376.
    [4] ZHANG Y, ZHOU W E, LI S H, et al. A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and metabolites in serum samples by ultra-high performance supercritical fluid chromatography[J]. Journal of Chromatography A, 2016, 1432:132-139.
    [5] MARTINEZ J L. Environmental pollution by antibiotics and by antibiotic resistance determinants[J]. Environmental Pollution, 2009, 157(11):2893-2902.
    [6] LUO Y, XU L, RYSZ M, et al. Occurrence and transport of tetracycline, sulfonamide, quinolone, and macrolide antibiotics in the Haihe River Basin, China[J]. Environmental Science & Technology, 2011, 45(5):1827-1833.
    [7] LERTPAITOONPAN W, ONG S K, MOORMAN T B. Effect of organic carbon and pH on soil sorption of sulfamethazine[J]. Chemosphere, 2009, 76(4):558-564.
    [8] ZHOU L J, YING G G, LIU S, et al. Occurrence and fate of eleven classes of antibiotics in two typical wastewater treatment plants in South China[J]. Science of the Total Environment, 2013, 452-453:365-376.
    [9] HIRSCH R, TERNES T, HABERER K, et al. Occurrence of antibiotics in the aquatic environment[J]. Science of the Total Environment, 1999, 225(1):109-118.
    [10] HALLER M Y, M LLER S R, MCARDELL C S, et al. Quantification of veterinary antibiotics (sulfonamides and trimethoprim) in animal manure by liquid chromatography-mass spectrometry[J]. Journal of Chromatography A, 2002, 952(1):111-120.
    [11] KISHIDA K, FURUSAWA N. Application of shielded column liquid chromatography for determination of sulfamonomethoxine, sulfadimethoxine, and their N4-acetyl metabolites in milk[J]. Journal of Chromatography A, 2004, 1028(1):175-177.
    [12] BALAKRISHNAN V K, EXALL K N, TOITO J M. The development of a microwave-assisted extraction method for the determination of sulfonamide antibiotics in sediments and soils[J]. Canadian Journal of Chemistry, 2014, 92(5):369-377.
    [13] TRAN N H, CHEN H J, REINHARD M, et al. Occurrence and removal of multiple classes of antibiotics and antimicrobial agents in biological wastewater treatment processes[J]. Water Research, 2016, 104:461-472.
    [14] PAPAGEORGIOU M, KOSMA C, LAMBROPOULOU D. Seasonal occurrence, removal, mass loading and environmental risk assessment of 55 pharmaceuticals and personal care products in a municipal wastewater treatment plant in Central Greece[J]. Science of the Total Environment, 2016, 543(Pt A):547-569.
    [15] HOFF R B, PIZZOLATO T M, PERALBA MDO C, et al. Determination of sulfonamide antibiotics and metabolites in liver, muscle and kidney samples by pressurized liquid extraction or ultrasound-assisted extraction followed by liquid chromatography-quadrupole linear ion trap-tandem mass spectrometry (HPLC-QqLIT-MS/MS)[J]. Talanta, 2015, 134:768-778.
    [16] GARCIA-GALAN M J, GARRIDO T, FRAILE J, et al. Simultaneous occurrence of nitrates and sulfonamide antibiotics in two ground water bodies of Catalonia (Spain)[J]. Journal of Hydrology, 2010, 383(1-2):93-101.
    [17] LI H, SUN H W, ZHANG J X, et al. Highly sensitive and simultaneous determination of sixteen sulphonamide antibiotics, four acetyled metabolites and trimethoprim in meat by rapid resolution liquid chromatography-tandem mass spectrometry[J]. Food Control, 2013, 31(2):359-365.
    [18] GARCIA-GALAN M J, DIAZ-CRUZ S, BARCELO D. Multiresidue trace analysis of sulfonamide antibiotics and their metabolites in soils and sewage sludge by pressurized liquid extraction followed by liquid chromatography-electrospray-quadrupole linear ion trap mass spectrometry[J]. Journal of Chromatography A, 2013, 1275:32-40.
    [19] SHAO B, CHEN D, ZHANG J, et al. Determination of 76 pharmaceutical drugs by liquid chromatography-tandem mass spectrometry in slaughterhouse wastewater[J]. Journal of Chromatography A, 2009, 1216(47):8312-8318.
    [20] NIESSEN W, MANINI P, ANDREOLI R. Matrix effects in quantitative pesticide analysis using liquid chromatography-mass spectrometry[J]. Mass Spectrometry Reviews, 2006, 25(6):881-899.
    [21] HUANG Y J, CHENG M M, LI W H, et al. Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method[J]. Analytical Methods, 2013, 5(15):3721-3731.
    [22] ZOU Y, ZHAO J, ZHANG J Z, et al. Matrix effects in the simultaneous determination of fenicol antibiotics in swine muscle and casings by ultra performance liquid chromatography-tandem mass spectrometry[J]. Analytical Methods, 2013, 5(20):5662-5668.
    [23] STAHNKE H, KITTLAUS S, KEMPE G, et al. Reduction of matrix effects in liquid chromatography-electrospray ionization-mass spectrometry by dilution of the sample extracts:how much dilution is needed?[J]. Analytical Chemistry, 2012, 84(3):1474-1482.
    [24] TONG L, LI P, WANG Y X, et al. Analysis of veterinary antibiotic residues in swine wastewater and environmental water samples using optimized SPE-LC/MS/MS[J]. Chemosphere, 2009, 74(8):1090-1097.
    [25] HOFF R, PIZZOLATO T M, DIAZ-CRUZ M S. Trends in sulfonamides and their by-products analysis in environmental samples using mass spectrometry techniques[J]. Trends in Environmental Analytical Chemistry, 2016, 9:24-36.
    [26] 许静, 王娜,孔德洋,等. 磺胺类药物在土壤中的降解性[J]. 环境化学, 2013, 32(12):2349-2356.

    XU J, WNAG N, KONG D Y, et al. Degradation of sulfonamides in soils[J]. Environmental Chemistry, 2013, 32(12):2349-2356(in Chinese).

    [27] 巫杨, MIKE W, RAI K, 等. 水体中磺胺甲恶唑和甲氧苄氨嘧啶的自然光降解[J]. 环境化学, 2013, 32(6):1081-1087.

    WU Y, MIKE W, RAI K, et al. Natural sunlight photodegradation of sulfamethoxazole and trimethoprim in aquatic environment[J]. Environmental Chemistry, 2013, 32(6):1081-1087(in Chinese).

    [28] GÖBEL A, THOMSEN A, MCARDELL C S, et al. Occurrence and sorption behavior of sulfonamides, macrolides, and trimethoprim in activated sludge treatment[J]. Environmental Science & Technology, 2005, 39(11):3981-3989.
    [29] ZHANG H, DU M M, JIANG H Y, et al. Occurrence, seasonal variation and removal efficiency of antibiotics and their metabolites in wastewater treatment plants, Jiulongjiang River Basin, South China[J]. Environmental Science:Processes & Impacts, 2015, 17(1):225-234.
    [30] GAO L H, SHI Y L, LI W H, et al. Occurrence of antibiotics in eight sewage treatment plants in Beijing, China[J]. Chemosphere, 2012, 86(6):665-671.
    [31] LI W H, SHI Y L, GAO L H, et al. Occurrence, distribution and potential affecting factors of antibiotics in sewage sludge of wastewater treatment plants in China[J]. Science of the Total Environment, 2013, 445-446:306-313.
    [32] GARCIA-GALAN M J, PETROVIC M, RODRIGUEZ-MOZAZ S, et al. Multiresidue trace analysis of pharmaceuticals, their human metabolites and transformation products by fully automated on-line solid-phase extraction-liquid chromatography-tandem mass spectrometry[J]. Talanta, 2016, 158:330-341.
    [33] PERIŠA M, BABIC S. Simultaneous determination of pharmaceuticals and some of their metabolites in wastewaters by high performance liquid chromatography with tandem mass spectrometry[J]. Journal of Separation Science, 2014, 37(11):1289-1296.
    [34] LE-MINH N, STUETZ R M, KHAN S J. Determination of six sulfonamide antibiotics, two metabolites and trimethoprim in wastewater by isotope dilution liquid chromatography/tandem mass spectrometry[J]. Talanta, 2012, 89:407-416.
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  • 收稿日期:  2017-12-08
  • 刊出日期:  2018-10-15
王大鹏, 张娴, 颜昌宙. 高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J]. 环境化学, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
引用本文: 王大鹏, 张娴, 颜昌宙. 高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J]. 环境化学, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
WANG Dapeng, ZHANG Xian, YAN Changzhou. Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804
Citation: WANG Dapeng, ZHANG Xian, YAN Changzhou. Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(10): 2143-2151. doi: 10.7524/j.issn.0254-6108.2017120804

高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物

  • 1.  中国科学院城市环境研究所, 城市环境与健康重点实验室, 厦门, 361021;
  • 2.  中国科学院大学, 北京, 100049
基金项目:

国家国际科技合作专项(2011DFB91710)资助.

摘要: 本文建立了一种检测污水及污泥中4种磺胺类抗生素及其相应的乙酰化代谢物的分析方法,包括磺胺嘧啶(SD)、磺胺甲基嘧啶(SM1)、磺胺二甲基嘧啶(SM2)、磺胺甲恶唑(SMZ)以及它们相对应的乙酰化代谢物.本文采用了固相萃取(SPE)的方法作为前处理,并优化了固相萃取条件.计算了样品中复杂的基质效应,并使用内标法来抵消基质效应.该方法在污水中检测限(LOD)和定量限(LOQ)分别为0.12-2.06 ng·L-1和0.43-5.12 ng·L-1,在污泥中分别为0.28-2.31 ng·g-1和0.88-6.77 ng·g-1,在污水和污泥中的回收率为分别为70.8%-130.6%,88.0%-129.2%.本方法成功应用于检测污水处理厂中的污水及污泥中的目标化合物.

English Abstract

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