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时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)

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黄梦璐, 张祯, 吴向阳. 时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)[J]. 环境化学, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
引用本文: 黄梦璐, 张祯, 吴向阳. 时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)[J]. 环境化学, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
shu
HUANG Menglu, ZHANG Zhen, WU Xiangyang. A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments[J]. Environmental Chemistry, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
Citation: HUANG Menglu, ZHANG Zhen, WU Xiangyang. A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments[J]. Environmental Chemistry, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
shu

时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)

  • 1.

    江苏大学环境与安全工程学院, 镇江, 212013

  • 基金项目:

    国家自然科学基金（21577051）资助.

A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments

  • 1.

    School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China

  • Fund Project: Supported by the National Natural Science Foundation of China(21577051).

  • 摘要: 本研究利用前期制备的四溴双酚A双（2-羟基乙基）醚（TBBPA DHEE）的多克隆抗体，构建了一种高灵敏的时间分辨荧光免疫分析法，用于检测环境中的四溴双酚A衍生物TBBPA DHEE.为提高分析方法的灵敏度，本研究对反应体系中有机溶剂（甲醇）、离子强度及缓冲体系pH等参数进行了优化.在最适条件下，本方法的最低检测限（LOD，基于F/F0=90%）与IC50分别为0.27 ng·mL-1及4.3 ng·mL-1.该方法具有较高的准确度（水样加标回收率为96%-120%；土样及生物加标回收率为75%-90%）.利用该方法对江苏省苏州某地区的环境样品进行了调查：在采集的56个样本中，13个样本检出了这种污染物.检出浓度为：水样0.5-2.7 ng·mL-1、土样0.6-1.6 ng·g-1，干重、生物样2.9-4.6 ng·g-1，湿重.本研究为首次针对环境中TBBPA DHEE污染状况的系统性分析.
    Abstract: A sensitive time-resolved fluoroimmunoassay (TRFIA) was developed using our previously produced polyclonal antibody for the determination of tetrabromobisphenol A bis(2-hydroxyetyl) ether (TBBPA DHEE), one of typical tetrabromobisphenol A (TBBPA) derivatives. The established method showed satisfactory sensitivity under the optimized conditions. The detection limit (LOD, based on 90% F/F0) and IC50 calculated from the standard curve were 0.27 and 4.3 ng·mL-1 for TBBPA DHEE, respectively. Using the TRFIA method, a systematic investigation was implemented at a halogenated flame retardants plant (main sampling site) in Suzhou, Jiangsu province, China. As a result, TBBPA DHEE was found in 5 of the 26 water samples with concentrations of 0.5-2.7 ng·mL-1, 5 of 20 soil samples with concentrations of 0.6-1.6 ng·g-1 (dry weight, dw), and 3 out of 10 fist samples with concentrations of 2.9-4.6 ng·g-1 (wet weight, ww). To our knowledge, this is the systematic investigation of TBBPA DHEE about its occurrence in the environment.
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  • 收稿日期:  2017-12-27
  • 刊出日期:  2018-10-15
黄梦璐, 张祯, 吴向阳. 时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)[J]. 环境化学, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
引用本文: 黄梦璐, 张祯, 吴向阳. 时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)[J]. 环境化学, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
shu
HUANG Menglu, ZHANG Zhen, WU Xiangyang. A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments[J]. Environmental Chemistry, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
Citation: HUANG Menglu, ZHANG Zhen, WU Xiangyang. A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments[J]. Environmental Chemistry, 2018, 37(10): 2124-2130. doi: 10.7524/j.issn.0254-6108.2017122701
shu

时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)

  • 1. 江苏大学环境与安全工程学院, 镇江, 212013
  • 收稿日期:  2017-12-27
基金项目:

国家自然科学基金（21577051）资助.

摘要: 本研究利用前期制备的四溴双酚A双（2-羟基乙基）醚（TBBPA DHEE）的多克隆抗体，构建了一种高灵敏的时间分辨荧光免疫分析法，用于检测环境中的四溴双酚A衍生物TBBPA DHEE.为提高分析方法的灵敏度，本研究对反应体系中有机溶剂（甲醇）、离子强度及缓冲体系pH等参数进行了优化.在最适条件下，本方法的最低检测限（LOD，基于F/F0=90%）与IC50分别为0.27 ng·mL-1及4.3 ng·mL-1.该方法具有较高的准确度（水样加标回收率为96%-120%；土样及生物加标回收率为75%-90%）.利用该方法对江苏省苏州某地区的环境样品进行了调查：在采集的56个样本中，13个样本检出了这种污染物.检出浓度为：水样0.5-2.7 ng·mL-1、土样0.6-1.6 ng·g-1，干重、生物样2.9-4.6 ng·g-1，湿重.本研究为首次针对环境中TBBPA DHEE污染状况的系统性分析.

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