包头南海湖冰封期沉积物细菌群落多样性
Diversity of bacterial communities in the sediment of Baotou Nanhai Lake in ice period
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摘要: 为了探究包头南海湖冰封期沉积物细菌群落多样性，采用高通量测序技术对不同湖区4个采样点表层沉积物细菌进行测定.结果显示，不同区域物种多样性有一定差异.从门水平来看，沉积物细菌主要为变形菌门（Proteobacteria）、绿弯菌门（Chloroflexi）、蓝藻门（Cyanobacteria）、拟杆菌门（Bacteroidetes）和酸杆菌门（Acidobacteria）；从属水平分析，优势类群集中在硫杆菌属（thiobacillus）、未命名厌氧绳菌科（norank-f-anaerolineaceae）、未分类绿弯菌科（unclassified-p-Chloroflexi）.对沉积物细菌群落与环境因子的关系进行冗余分析，结果表明，TN、NH3-N、C/N对沉积物细菌群落结构影响较大.thiobacillus在南海湖冰封期沉积物的生物地球化学循环中扮演着重要的角色，对预防黑臭水体发生具有重要作用，应在以后研究中得到更多关注.Abstract: To explore the diversity of bacterial communities in the sediment in Baotou Nanhai Lake in ice period,the surficial sedimentary bacterial of 4 sampling sites in different areas was sequenced using high-throughput sequencing approach. The result showed that the diversity of species in different areas was quite different. At the phyla level, Proteobacteria, Chloroflexi, Cyanobacteria, Bacteroidetes and Acidobacteria were dominant. Thiobacillus,norank-f-anaerolineaceae,unclassified-p-Chloroflexi were dominant bacteria at the genus level. The redundancy analysis(RDA) for the relationship between bacterial community and environmental factors revealed that the TN,NH3-N and C/N had great influence on the structure of sedimentary bacterial community. Thiobacillus played an important role in biogeochemical cycles of sediments in Nanhai Lake in ice period and the provention of occurrence of black and odorous water. More attention should be paid to the thiobaciuus in future studies to provide theoretical basis and basic data for water pollution control in Nanhai Lake.
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Key words:
- bacteria /
- community structure /
- high-throughput sequencing /
- Nanhai Lake /
- ice period /
- Baotou
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