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碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定

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贾真真, 解静芳, 王振涛, 姜洪进, 任浦慧. 碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定[J]. 环境化学, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
引用本文: 贾真真, 解静芳, 王振涛, 姜洪进, 任浦慧. 碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定[J]. 环境化学, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
shu
JIA Zhenzhen, XIE Jingfang, WANG Zhentao, JIANG Hongjin, REN Puhui. Rapid determination of chloramphenicol by carbon nanotubes and molecularly imprinted polymer modified glassy carbon electrode[J]. Environmental Chemistry, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
Citation: JIA Zhenzhen, XIE Jingfang, WANG Zhentao, JIANG Hongjin, REN Puhui. Rapid determination of chloramphenicol by carbon nanotubes and molecularly imprinted polymer modified glassy carbon electrode[J]. Environmental Chemistry, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
shu

碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定

  • 1.

    山西大学环境与资源学院, 太原, 030006

  • 基金项目:

    国家大气重污染成因与治理攻关项目（DQGG-05-11）和国家公益性行业（农业）科研专项（201103024）资助.

Rapid determination of chloramphenicol by carbon nanotubes and molecularly imprinted polymer modified glassy carbon electrode

  • 1.

    College of Environment and Resource, Shanxi University, Taiyuan, 030006, China

  • Fund Project: Supported by the National Project on Causes and Control of Heavy Air Pollution(DQGG-05-11) and National Public Welfare Industry (Agriculture) Research Projects(201103024).

  • 摘要: 为了研究水环境中氯霉素（CAP）的快速检测方法，用单壁碳纳米管（SWCNTs）-纳米铜（CuNPs）复合物修饰玻碳电极（GCE），并结合分子印迹（MIP）技术，先后采用循环伏安法（CV）进行电极导电性测试，线性伏安法（LSV）对实验条件进行优化，差分脉冲伏安法（DPV）研究水溶液中CAP的电化学行为.研究结果表明，与GCE电极相比，修饰电极上CAP氧化还原电流信号明显增强，表明修饰电极对CAP具有较好的选择性，能加速电子传递.同时对洗脱液类型、缓冲溶液pH、扫描速度、富集时间及修饰物的负载量等实验条件进行了优化.在最佳条件下，发现CAP还原峰电流大小与其浓度在5-500 nmol·L-1范围内呈现良好的线性关系，线性方程为I（μA）=0.9204C+41.285（R2=0.9984），检出限为4.8 nmol·L-1（S/N=3），且该修饰电极具有良好的重现性和稳定性.将该修饰电极应用于实际样品分析时，氯霉素眼药水的加标回收率为95.1%-102.5%，实际地表水样的加标回收率为96.5%-102.1%.
    Abstract: In order to study the rapid detection method of chloramphenicol (CAP) in water environment, single\walled carbon nanotubes (SWCNTs) -nano-copper (CuNPs) composites were used to modify glassy carbon electrodes (GCE) combined with molecular imprinting polymer (MIP) technology. Cyclic voltammetry (CV) was used to conduct electrode conductivity test. Linear scan voltammetry (LSV) was used to optimize the experimental conditions. Differential pulse voltammetry (DPV) was used to study the electrochemical behavior of CAP in aqueous solution. The results show that the CAP redox current signal of the modified electrode was significantly enhanced compared with the GCE electrode, which indicated that the modified electrode has strong selectivity to CAP and accelerated electron transport. At the same time, the experimental conditions were optimized, such as the eluent type, the pH value of the buffer solution, the scanning speed, the enrichment time and the loading of the modifier. Under the optimal conditions, it was found that the peak current of CAP reduction showed a good linear relationship with the concentration in the range of 5-500 nmol·L-1. The linear equation is I(μA)=0.9204C + 41.285 (R2=0.9984). The limit is 4.8 nmol·L-1 (S/N=3), and the modified electrode has the good reproducibility and stability. When the modified electrode was applied to the actual sample analysis, the spiked recovery rate of chloramphenicol eye drops was 95.1%-102.5%, and spike recovery of the actual surface water sample was 96.5%-102.1%.
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  • 收稿日期:  2018-08-06
  • 刊出日期:  2019-03-15
贾真真, 解静芳, 王振涛, 姜洪进, 任浦慧. 碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定[J]. 环境化学, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
引用本文: 贾真真, 解静芳, 王振涛, 姜洪进, 任浦慧. 碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定[J]. 环境化学, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
shu
JIA Zhenzhen, XIE Jingfang, WANG Zhentao, JIANG Hongjin, REN Puhui. Rapid determination of chloramphenicol by carbon nanotubes and molecularly imprinted polymer modified glassy carbon electrode[J]. Environmental Chemistry, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
Citation: JIA Zhenzhen, XIE Jingfang, WANG Zhentao, JIANG Hongjin, REN Puhui. Rapid determination of chloramphenicol by carbon nanotubes and molecularly imprinted polymer modified glassy carbon electrode[J]. Environmental Chemistry, 2019, 38(3): 513-521. doi: 10.7524/j.issn.0254-6108.2018080601
shu

碳纳米管与分子印迹结合修饰玻碳电极应用于氯霉素的快速测定

  • 1. 山西大学环境与资源学院, 太原, 030006
  • 收稿日期:  2018-08-06
基金项目:

国家大气重污染成因与治理攻关项目（DQGG-05-11）和国家公益性行业（农业）科研专项（201103024）资助.

摘要: 为了研究水环境中氯霉素（CAP）的快速检测方法，用单壁碳纳米管（SWCNTs）-纳米铜（CuNPs）复合物修饰玻碳电极（GCE），并结合分子印迹（MIP）技术，先后采用循环伏安法（CV）进行电极导电性测试，线性伏安法（LSV）对实验条件进行优化，差分脉冲伏安法（DPV）研究水溶液中CAP的电化学行为.研究结果表明，与GCE电极相比，修饰电极上CAP氧化还原电流信号明显增强，表明修饰电极对CAP具有较好的选择性，能加速电子传递.同时对洗脱液类型、缓冲溶液pH、扫描速度、富集时间及修饰物的负载量等实验条件进行了优化.在最佳条件下，发现CAP还原峰电流大小与其浓度在5-500 nmol·L-1范围内呈现良好的线性关系，线性方程为I（μA）=0.9204C+41.285（R2=0.9984），检出限为4.8 nmol·L-1（S/N=3），且该修饰电极具有良好的重现性和稳定性.将该修饰电极应用于实际样品分析时，氯霉素眼药水的加标回收率为95.1%-102.5%，实际地表水样的加标回收率为96.5%-102.1%.

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