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程铭, 张波, 何义亮, 沈根祥, 胡双庆. 太浦河金泽水源地抗生素抗性基因赋存特征[J]. 环境化学, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
引用本文: 程铭, 张波, 何义亮, 沈根祥, 胡双庆. 太浦河金泽水源地抗生素抗性基因赋存特征[J]. 环境化学, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
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CHENG Ming, ZHANG Bo, HE Yiliang, SHEN Genxiang, HU Shuangqing. Occurrence and distribution of antibiotic resistance genes in Jinze Reservoir and surrounding area of Taipu River[J]. Environmental Chemistry, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
Citation: CHENG Ming, ZHANG Bo, HE Yiliang, SHEN Genxiang, HU Shuangqing. Occurrence and distribution of antibiotic resistance genes in Jinze Reservoir and surrounding area of Taipu River[J]. Environmental Chemistry, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
shu

太浦河金泽水源地抗生素抗性基因赋存特征

  • 1. 上海交通大学环境科学与工程学院, 上海, 200240;

  • 2. 上海市环境科学研究院, 上海, 200233

    • 通讯作者: 张波, E-mail: zhangbo214@sjtu.edu.cn ; 
  • 基金项目:

    国家水体污染控制与治理科技重大专项（2017ZX07207002-05）资助.

Occurrence and distribution of antibiotic resistance genes in Jinze Reservoir and surrounding area of Taipu River

  • 1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China;

  • 2. Shanghai Institute of Environmental Sciences, Shanghai, 200233, China

    • Corresponding author: ZHANG Bo, zhangbo214@sjtu.edu.cn ; 
  • Fund Project: Supported by National Major Project on Water Pollution Control and Management Science and Technology(2017ZX07207002-05).

  • 摘要: 金泽水库是上海市2016年新建水库，对该水源地的抗生素抗性基因（Antibiotic resistance genes，ARGs）污染水平进行监测分析对保证其水质安全及采取有效防控措施具有重要意义.本研究采用高通量荧光定量反应及实时荧光定量PCR对研究区域的8个采样点进行了为期1年的监测.结果表明，该水源地全年共检出84—159种ARGs，其中检出的多重抗药类、氨基糖苷类和磺胺类3类ARGs的相对及绝对丰度均最高.春季ARGs的检出丰度最高而冬季最低，并且发现水库对ARGs有富集作用.该水源地水体中大多数类别ARGs与MGEs（mobile genetic elements）存在显著正相关关系；并且存在基因盒模块，其中含有intⅠ-1（clinical）、intⅠ-1LC及tnpA-04，表明水体中ARGs的传播与富集与MGEs密切相关.
    Abstract: Jinze Reservoir is a new reservoir in Shanghai. It is of great significance to monitor and analyze the levels of antibiotic resistance genes (ARGs) in this drinking water source for the safety of water quality. This work investigated the occurrence of ARGs at the 8 sampling sites in and around the Jinze Reservoir for one year by using high-throughput fluorescence quantitative reaction and real-time fluorescentce quantitative PCR. The results show that 84-159 ARGs were detected in the water source, and the relative and absolute abundances of the multi-drug resistant, such as aminoglycoside and sulfonamide ARGs were the highest.The abundance of ARGs was the highest in spring and the lowest in winter, and it was found that the reservoir had an enrichment effect on ARGs. There is a significant positive correlation between most types of ARGs and MGEs in the water source. There is also a gene cassette module containing intⅠ-1 (clinical), intⅠ-1LC and tnpA-04, indicating that the transfer and enrichment of ARGs are closely relavant to MGEs.
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  • [1] BERGLUND B. Environmental dissemination of antibiotic resistance genes and correlation to anthropogenic contamination with antibiotics[J]. Infection Ecology Epidemiology, 2015, 5: 28564-28574.
    [2] ZHANG Q Q, YING G G, PANG C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: Source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental Science Technology, 2015, 49(11): 6772-6782.
    [3] KUMAR K, GUPTA S C, CHANDER Y, et al. Antibiotic use in agriculture and its impact on the terrestrial environment[J]. Advances in Agronomy, 2005, 87(5): 1-54.
    [4] MA R, WANG B, LU S, et al. Characterization of pharmaceutically active compounds in Dongting Lake, China: Occurrence, chiral profiling and environmental risk[J]. Science of the Total Environment, 2016, 557-558: 268-275.
    [5] CARVALHO I T, SANTOS L. Antibiotics in the aquatic environments: A review of the European scenario[J]. Environment International, 2016, 94: 736-757.
    [6] PRUDEN A, PEI R, STORTEBOOM H, et al. Antibiotic resistance genes as emerging contaminants: Studies in northern Colorado[J]. Environmental Science Technology, 2006, 40(23): 7445-7450.
    [7] XU Y, GUO C, LUO Y, et al. Occurrence and distribution of antibiotics, antibiotic resistance genes in the urban rivers in Beijing, China[J]. Environmental Pollution, 2016, 213: 833-840.
    [8] YIN Q, YUE D, PENG Y, et al. Occurrence and distribution of antibiotic-resistant bacteria and transfer of resistance genes in Lake Taihu[J]. Microbes Environments, 2013, 28(4): 479-486.
    [9] WANG C, GU X C, ZHANG S, et al. Characterization of antibiotic-resistance genes in antibiotic resistance escherichia coli isolates from a lake[J]. Archives of Environmental Contamination Toxicology, 2013, 65(4): 635-641.
    [10] JIA J, GUAN Y, CHENG M, et al. Occurrence and distribution of antibiotics and antibiotic resistance genes in Ba River, China[J]. Science of the Total Environment, 2018, 642: 1136-1144.
    [11] FLORIAN T, THIERRY A, WALTER W, et al. Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland)[J]. Chemosphere, 2012, 86(5): 468-476.
    [12] 任红. 金泽水库[J]. 中国三峡, 2018, (2): 72-75.REN H. Jinze Reservoir[J]. China Three Gorges, 2018

    , (2): 72-75(in Chinese).

    [13] WANG F H, QIAO M, SU J Q, et al. High throughput profiling of antibiotic resistance genes in urban park soils with reclaimed water irrigation[J]. Environmental Science Technology, 2014, 48(16): 9079-9085.
    [14] TOREY L, TIMOTHY A J, HEATHER K A, et al. In-feed antibiotic effects on the swine intestinal microbiome[J]. Gut Microbes, 2012, 109(5): 1691-1696.
    [15] 黄福义, 安新丽, 陈青林, 等. 梅花鹿养殖场抗生素抗性基因分布特征[J]. 环境科学, 2016, 37(11): 4402-4409.

    HUANG F Y, AN X L, CHEN Q L, et al. Distribution characteristics of antibiotic resistance genes in sika deer farm[J]. Environmental Science, 2016, 37(11): 4402-4409(in Chinese).

    [16] CHEN Y, SU J Q, ZHANG J, et al. High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system[J]. Water Research, 2019, 149: 179-189.
    [17] OUYANG W Y, HUANG F Y, ZHAO Y, et al. Increased levels of antibiotic resistance in urban stream of Jiulongjiang River, China[J]. Applied Microbiology Biotechnology, 2015, 99(13): 5697-5707.
    [18] ZHU Y G, ZHAO Y, LI B, et al. Continental-scale pollution of estuaries with antibiotic resistance genes[J]. Nature Microbiology, 2017, 2: 16270.
    [19] ZHENG J, GAO R, WEI Y, et al. High-throughput profiling and analysis of antibiotic resistance genes in East Tiaoxi River, China[J]. Environmental Pollution, 2017, 230: 648-654.
    [20] JIANG L, HU X, XU T, et al. Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources, Shanghai, China[J]. Science of the Total Environment, 2013, 458-460(3): 267-272.
    [21] 江月. 长江下游某水源型水库抗生素与抗性基因赋存特征与风险评价[D]. 上海: 上海交通大学, 2018.JIANG Y.The occurrence and risk assessment of antibiotic and antibiotic resistantgenes in a reservoir located at Yangtze River[D]. Shanghai: Shanghai Jiao Tong University, 2018(in Chinese).
    [22] 庄榆佳, 赵忆, 苏建强, 等. 抗生素抗性基因在养殖废水中的分布与去除[J]. 环境化学, 2017, 36(11): 2311-2318.

    ZHUANG Y J, ZHAO Y, SU J Q, et al. Distribution and removal of antibiotic resistance genes in swine wastewater[J]. Environmental Chemistry, 2017, 36(11): 2311-2318(in Chinese).

    [23] 杨继平, 邱志刚, 袁兆康,等. 天津海河流域抗生素抗性基因分布特征及与指示微生物的关系[J]. 环境与健康杂志, 2017, 34(4): 313-316.

    YANG J P, QIU Z G, YUAN Z K, et al. Distribution of antibiotic resistance genes and their relationship with indicator microorganisms in Haihe River Basin, Tianjin[J]. Journal of Environment and Health, 2017, 34(4): 313-316(in Chinese).

    [24] STOLL C, SIDHU J P, TIEHM A, et al. Prevalence of clinically relevant antibiotic resistance genes in surface water samples collected from Germany and Australia[J]. Environmental Science Technology, 2012, 46(17): 9716-9726.
    [25] HAMELIN K, BRUANT G, EL-SHAARAWI A, et al. A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters[J]. Applied Environmental Microbiology, 2006, 72(6): 4200-4206.
    [26] 郑吉. 流域水体及饮用水处理过程中抗生素抗性基因的行为特征[D]. 杭州:浙江大学, 2018.ZHENG J. The behavior of antibiotic resistence genes in river basin and drinking water treatment[D].Hangzhou: Zhejiang Universiry, 2018(in Chinese).
    [27] 陈奕涵. "河流-水库"系统水环境典型污染物赋存特征的研究-以东江源区为例[D]. 上海:上海交通大学, 2018.CHEN Y H. Occurrence characteristics of typical pollutants in the auqtic environment of river-reservoir system: A case stduy in the headwater region of dongjiang river[D]. Shanghai: Shanghai Jiao Tong Universiry, 2018(in Chinese).
    [28] KOCZURA R, MOKRACKA J, JABŁOŃSKA L, et al. Antimicrobial resistance of integron-harboring Escherichia coli isolates from clinical samples, wastewater treatment plant and river water[J]. Science of the Total Environment, 2012, 414(414): 680-685.
    [29] HOLMES A J, GILLINGS M R, NIELD B S, et al. The gene cassette metagenome is a basic resource for bacterial genome evolution[J]. Environmental Microbiology, 2010, 5(5): 383-394.
    [30] NEMEC A, KRIZOVA L, MAIXNEROVA M, et al. Multidrug-resistant epidemic clones among bloodstream isolates of Pseudomonas aeruginosa in the Czech Republic[J]. Research in Microbiology, 2010, 161(3): 234-242.
    [31] FARKAS A, BUTIUC-KEUL A, CIATARÂŞ D, et al. Microbiological contamination and resistance genes in biofilms occurring during the drinking water treatment process[J]. Science of the Total Environment, 2013, 443(1): 932-938.
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  • 收稿日期:  2018-12-28
程铭, 张波, 何义亮, 沈根祥, 胡双庆. 太浦河金泽水源地抗生素抗性基因赋存特征[J]. 环境化学, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
引用本文: 程铭, 张波, 何义亮, 沈根祥, 胡双庆. 太浦河金泽水源地抗生素抗性基因赋存特征[J]. 环境化学, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
shu
CHENG Ming, ZHANG Bo, HE Yiliang, SHEN Genxiang, HU Shuangqing. Occurrence and distribution of antibiotic resistance genes in Jinze Reservoir and surrounding area of Taipu River[J]. Environmental Chemistry, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
Citation: CHENG Ming, ZHANG Bo, HE Yiliang, SHEN Genxiang, HU Shuangqing. Occurrence and distribution of antibiotic resistance genes in Jinze Reservoir and surrounding area of Taipu River[J]. Environmental Chemistry, 2019, (11): 2405-2414. doi: 10.7524/j.issn.0254-6108.2018122802
shu

太浦河金泽水源地抗生素抗性基因赋存特征

    通讯作者: 张波, E-mail: zhangbo214@sjtu.edu.cn ; 
  • 1. 上海交通大学环境科学与工程学院, 上海, 200240;
  • 2. 上海市环境科学研究院, 上海, 200233
  • 收稿日期:  2018-12-28
基金项目:

国家水体污染控制与治理科技重大专项（2017ZX07207002-05）资助.

摘要: 金泽水库是上海市2016年新建水库，对该水源地的抗生素抗性基因（Antibiotic resistance genes，ARGs）污染水平进行监测分析对保证其水质安全及采取有效防控措施具有重要意义.本研究采用高通量荧光定量反应及实时荧光定量PCR对研究区域的8个采样点进行了为期1年的监测.结果表明，该水源地全年共检出84—159种ARGs，其中检出的多重抗药类、氨基糖苷类和磺胺类3类ARGs的相对及绝对丰度均最高.春季ARGs的检出丰度最高而冬季最低，并且发现水库对ARGs有富集作用.该水源地水体中大多数类别ARGs与MGEs（mobile genetic elements）存在显著正相关关系；并且存在基因盒模块，其中含有intⅠ-1（clinical）、intⅠ-1LC及tnpA-04，表明水体中ARGs的传播与富集与MGEs密切相关.

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