高级搜索

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

,  ,  , 

downloadPDF

上一篇

下一篇

梁浩花, 王亚娟, 陶红, 张小红. 一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
引用本文: 梁浩花, 王亚娟, 陶红, 张小红.

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu
LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong. Isolation, identification and degradation characteristics of a three phthalates degrading bacterium[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
Citation: LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong.

Isolation, identification and degradation characteristics of a three phthalates degrading bacterium

[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

  • 1. 宁夏大学资源环境学院, 银川, 750021;

  • 2. 宁夏大学经济管理学院, 银川, 750021;

  • 3. 宁夏(中阿)旱区资源评价与环境调控重点实验室, 银川, 750021

    • 通讯作者: 王亚娟, E-mail: 358437013@qq.com ; 
  • 基金项目:

    宁夏回族自治区重点研发计划项目（2016KJHM29）资助.

Isolation, identification and degradation characteristics of a three phthalates degrading bacterium

  • 1. College of Resources and Environment, Ningxia University, Yinchuan, 750021, China;

  • 2. College of Economics and Management, Ningxia University, Yinchuan, 750021, China;

  • 3. Key Laboratory(China-Arab) of Resource Evaluation and Environmental Regulation of Arid Region in Ningxia, Yinchuan, 750021, China

    • Corresponding author: WANG Yajuan, 358437013@qq.com ; 
  • Fund Project: Supported by the Key Research Plan Program of the Ningxia Hui Autonomous Region(2016KJHM29).

  • 摘要:

    选择邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二丁酯（DnBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）作为目标污染物，采用富集驯化法从设施菜地土壤中筛选出1株可同时降解DMP、DnBP和DEHP的细菌MB1.经形态、生理生化特征及16S rDNA序列分析，初步鉴定为微杆菌属（Microbacterium sp.）.通过正交试验研究了该菌株的最优降解条件以及最优条件下该菌株的生长曲线和降解曲线，最后在培养条件下研究了该菌株对人工污染土壤中邻苯二甲酸酯的降解特性.结果表明，菌株MB1的最优降解条件为：pH值为8，温度为25℃，接菌量为5%，每种邻苯二甲酸酯浓度为300 mg·L-1.在此最优条件下该菌株呈S型曲线增长，7 d后无机盐培养液中DMP、DnBP和DEHP的降解率分别为99.62%%、99.65%和55.26%.人工污染土壤中空白试验和投加菌株试验结果为：在不添加菌液的处理中，灭菌土壤21 d时DMP、DnBP和DEHP的降解率分别为3.86%、4.19%和2.01%；未灭菌土壤21 d时对DMP、DnBP和DEHP的降解率分别为4.82%、5.99%和3.44%.在添加菌液的处理中，21 d时土壤灭菌处理中DMP、DnBP和DEHP的降解率分别达94.45%、95.65%和39.21%；而土壤未灭菌处理中DMP、DnBP和DEHP的降解率分别达94.93%、95.99%和41.16%.该结果表明：土壤中土著微生物仅能降解微量PAEs，菌株MB1对土壤中DMP、DnBP和DEHP等3种PAEs污染物具有较为高效的降解能力，未灭菌土壤中邻苯二甲酸酯的降解效果略高于灭菌土壤.

    Abstract:

    A strain, named MB1, capable of simultaneously degrading dimethyl phthalate(DMP),dibutyl phthalate (DnBP) and di-(2-ethylhexyl) phthalate (DEHP) and was isolated by culture enrichment method from facility vegetable soil. Based on the morphology, physiological characteristic and 16S rDNA sequence analysis, MB1 was identified as Microbacterium sp. and the optimal degradation conditions were studied by orthogonal experiment design. And growth curve, degradation curve of strain MB1 were investigated under optimal degradation conditions. Finally, the strain MB1 to phthalic acid esters degradation characteristics in artificially contaminated soil was investigated in culture incubation. The results showed that the optimized degradation condition was pH=8, temperature 25℃, inoculum size (V/V) 5% and 300 mg·L-1 of original PAEs concentration. Under the optimal condition, the strain presented shaped growth curve, and the degradation rate of DMP, DnBP and DEHP were 99.62%, 99.65% and 55.26% in mineral salt medium after 7 days, respectively. The degradation rates of DMP, DnBP and DEHP were 3.86%, 4.19% and 2.01% in the sterilized soil and 4.82%, 5.99% and 3.44% in the unsterilized soil without adding strain after 21 days, respectively. The degradation rate of DMP, DnBP and DEHP were 94.45%, 95.65% and 39.21% in sterilized soil and 94.93%, 95.99% and 41.16% in unsterilized soil by adding strain after 21 days, respectively. The results showed that:The strain MB1 had a relatively high degradation capacity for DMP, DnBP and DEHP in soil, and indigenous microbes degraded only a small amount of PAEs in soil. Degradation of phthalates by the strain MB1 was slightly higher in the unsterilized soil than in the sterilized soil.

  • 加载中
  • [1] WANG J, CHEN G, CHRISTIE P, et al. Occurrence and risk assessment of phthalate esters (PAEs) in vegetablesand soils of suburban plastic film greenhouses[J]. Science of the Total Environment, 2015,523:129-137.
    [2] 杨志豪, 何明靖, 杨婷,等. 邻苯二甲酸酯在重庆市城市土壤中的污染分布特征及来源分析[J]. 环境科学, 2018, 39(7):1-16.

    YANG Z H, HE M J, YANG T, et al. Occurrence and distribution of the phthalate esters in urban soils of Chongqing City[J]. Environmental Science, 2018, 39(7):1-16(in Chinese).

    [3] FENG N X, YU J, MO C H, et al. Biodegradation of di-n-butyl phthalate (DBP) by a novel endophytic Bacillus megaterium strain YJB3.[J]. Science of the Total Environment, 2017, 616:117-127.
    [4] WANG J, ZHANG MY, CHEN T,et al. Isolation and identification of a di-(2-Ethylhexyl) phthalate-degrading bacterium and its role in the bioremediation of a contaminated soil[J]. Pedosphere, 2015, 25(2):202-211.
    [5] 张可, 陈强, 陈伟,等. 邻苯二甲酸二甲酯(DMP)降解菌的分离鉴定及降解特性[J]. 安全与环境学报, 2017, 17(2):645-649.

    ZHANG K, CHEN Q, CHEN W, et al. On the isolation, identification and characterization of DMP-degrading strain[J]. Journal of Safety and Environment, 2017, 17(2):645-649(in Chinese).

    [6] 金德才, 吴学玲, 梁任星,等. 一株DMP降解菌的分离鉴定及其降解特性[J]. 微生物学通报, 2009, 36(9):1318-1323.

    JIN D C, WU X L, LIANG R X, et al. Isolation, identification and degradation characteristics of a DMP-degrading strain[J]. Microbiology China, 2009,36(9):1318-1323(in Chinese).

    [7] 王冬莹, 吴文成, 李霞,等. 一株能同时降解邻苯二甲酸二丁酯(DBP)和氰戊菊酯真菌的分离、鉴定及降解特性的初步研究[J]. 环境科学学报, 2015, 35(11):3493-3499.

    WANG D Y, WU W C, LI X, et al. Isolation, identification and degradation characteristics of a dibutyl phthalate and fenvalerate degrading fungal strain[J].Acta Scientiae Circumstantiae, 2015, 35(11):3493-3499(in Chinese).

    [8] 李方方, 宋建, 吕律,等. DBP高效降解菌群的富集及其降解特性研究[J]. 环境科学学报, 2017, 37(7):2579-2585.

    LI F F, SONG J, LÜ L, et al. Enrichment of a highly efficient DBP-degrading bacterium consortium and its degradation characters[J]. Acta Scientiae Circumstantiae, 2017, 37(7):2579-2585(in Chinese).

    [9] 高俊贤, 刘琦, 连梓竹, 等. 一株DBP高效降解菌的分离、鉴定与降解性能[J]. 环境工程学报, 2016, 10(3):1521-1526.

    GAO J X, LIU Q, LIAN Z Z, et al. Isolation and identification of highly efficient DBP-degradingstrain and its degradation study[J]. Chinese Journal of Environmental Engineering, 2016, 10(3):1521-1526(in Chinese).

    [10] 金雷. 1株DBP高效降解菌H-2的分离鉴定及其降解特性研究[J]. 食品科学, 2014, 35(15):202-206.

    JIN L. Isolation and characterization of a DBP-degrading strain H-2 and its degrading characteristics[J]. Food Science,2014, 35(15):202-206(in Chinese).

    [11] 李建龙, 邵向丽, 刘书亮, 等. 邻苯二甲酸二丁酯降解菌株的筛选鉴定及其降解特性研究[J]. 现代食品科技, 2014, 30(10):108-114.

    LI J L, SHAO X L, LIU S L, et al. Isolation and identification of dibutyl phthalate-degrading strain and its degradation characteristics[J]. Modern Food Science and Technology, 2014, 30(15):202-206(in Chinese).

    [12] JIN D, BAI Z, CHANG D, et al. Biodegradation of di-n-butyl phthalate by an isolated Gordonia sp. strain QH-11:Genetic identification and degradation kinetics[J]. Journal of Hazardous Materials, 2012, 221-222(2):80-85.
    [13] 陈学斌. DEHP降解菌的分离及其与玉米联合修复受污染土壤[D].广州:暨南大学, 2017. CHEN X B. Isolation of Di(2-ethylhexyl) phthalate degrading bacteria and its combined remediation with maize for contaminated soil[D]. Guangzhou:Jinan University, 2017(in Chinese).
    [14] 姚雪松. 邻苯二甲酸二(2-乙基己基)酯降解菌的分离鉴定及降解特性研究[D]. 牡丹江:牡丹江师范学院, 2016. YAO X S. Isolation, identification of a DEHP-degrading bacterium and its biodegradation characteristics[D]. Mudanjiang:Mudanjiang Normal College, 2016(in Chinese).
    [15] 高静静, 陈丽玮, 王宜青, 等. 一株邻苯二甲酸二(2-乙基己基)酯(DEHP)高效降解菌的筛选及其降解特性[J]. 环境化学, 2016, 35(11):2362-2369.

    GAO J J, CHEN L W, WANG Y Q, et al. Isolation, identification of a DEHP-degrading bacterium and its high effective biodegradation characteristics[J]. Environmental Chemistry, 2016, 35(11):2362-2369(in Chinese).

    [16] 严佳丽, 陈湖星, 杨杨, 等. 一株高效DEHP降解菌的分离、鉴定及其降解特性[J]. 微生物学通报, 2014, 41(8):1532-1540.

    YAN J L, CHEN H X, YANG Y, et al. Isolation and characterization of a highly DEHP-degrading bacterium[J]. Microbiology China, 2014, 41(8):1532-1540(in Chinese).

    [17] 刘时旸. 邻苯二甲酸二正戊酯高效降解菌的筛选、降解特性及其降解途径研究[D]. 南京:南京农业大学, 2015. LIU S C. Screening of highly efficient degradation bacteria of Di-n-amyl phthalate and its degradation characteristics[D]. Nanjing:Nanjing Agricultural University, 2015(in Chinese).
    [18] 杨婧. 邻苯二甲酸酯降解菌的降解特性与土壤应用研究[D]. 广州:华南理工大学,2018. YANG J. Characterization of phthalate-degrading bacterial strain and its application in phthalate-contaminated soil[D]. Guangzhou:South China University of Technology, 2018(in Chinese).
    [19] 周长健. 高效DEHP降解菌的筛选鉴定及其土壤模拟修复初探[D]. 哈尔滨:东北农业大学,2016. ZHOU C J. Solation of an efficient Di-(2-ethylhexyl) phthalate degrading bacteria and preliminary study of the soil remediation[D]. Haerbin:Dongbei Agricultural University, 2016(in Chinese).
    [20] 梁浩花, 王亚娟, 陶红, 等. 银川市东郊设施蔬菜基地土壤中邻苯二甲酸酯污染特征及健康风险评价[J].环境科学学报, 2018, 38(9):3703-3713.

    LIANG H H, WANG Y J, TAO H, et al.Pollution characteristics of phthalate esters(PAEs) in soils of facility vegetablebases and health risk assessment in eastern suburb of Yinchuan[J]. Acta Scientiae Circumstantiae, 2018, 38(9):3703-3713(in Chinese).

    [21] 褚娇艳, 刘腾飞, 张汉虞, 等. 一株邻苯二甲酸二丁酯降解菌的筛选及其降解特性[J]. 微生物学通报, 2017, 44(7):1555-1562.

    ZHE Y J, LIU T F, ZHANG H Y, et al. Screening and characterization of a diphthalate-degrading bacterium[J]. Microbiology China, 2017, 44(7):1555-1562(in Chinese).

    [22] 饶潇潇, 王建超, 周震峰. 花生对土壤中邻苯二甲酸酯的吸收累积特征[J]. 环境科学学报, 2017, 37(4):1531-1538.

    RAO X, WANG J C, ZHOU Z F, et al. Characteristics of uptake and accumulation of phthalic acid esters in soil by peanut (Arachis hypogaea)[J]. ActaScientiae Circumstantiae, 2017, 37(4):1531-1538(in Chinese).

    [23] 张颖, 王丽华, 陈艺洋, 等. 一株DBP高效降解菌的筛选及降解特性研究[J]. 东北农业大学学报, 2016, 47(8):46-54.

    ZHANG Y, WANG L H, CHEN Y Y, et al. Study on isolation and identification of a Di-n-butyl phthalate (DBP) strain and its degradation characteristics[J]. Journal of Northeast Agricultural University, 2016, 47(8):46-54(in Chinese).

    [24] 杜欢. 邻苯二甲酸酯降解菌的筛选、降解特性及其在土壤修复中的应用研究[D]. 广州:暨南大学, 2016. DU H. Isolation and characterization of phthalate-degrading bacteria and its application in soil remediation[D]. Guangzhou:Jinan University, 2016(in Chinese).
    [25] XU J, LU Q, TOLEDO R A D, et al. Degradation of di-2-ethylhexyl phthalate (DEHP) by an indigenous isolate Acinetobacter sp. SN13[J]. International Biodeterioration & Biodegradation, 2017, 117:205-214.
    [26] ZHAO H M, DU H, LIN J, et al. Complete degradation of the endocrine disruptor di-(2-ethylhexyl) phthalate by a novel Agromyces sp. MT-O strain and its application to bioremediation of contaminated soil.[J]. Science of the Total Environment, 2016, 562:170-178.
    [27] HUANG B, LI D, YANG Y. Joint toxicity of two phthalates with waterborne copper to daphnia magna and photobacterium phosphoreum[J]. Bulletin of Environmental Contamination & Toxicology, 2016, 97(3):380-387.
    [28] 穆希岩, 李成龙, 黄瑛, 等. 两种邻苯二甲酸酯类污染物对斑马鱼胚胎发育的影响[J]. 中国环境科学,2017,37(9):3566-3575.

    MU Y X, LI C L, HUANG Y, et al. Effects of two phthalate esters on zebrafish embryos[J]. China Environmental Science, 2017, 37(9):3566-3575(in Chinese).

    [29] 李玉双, 陈琳, 郭倩, 等. 沈阳市新民设施农业土壤中邻苯二甲酸酯的污染特征[J]. 农业环境科学学报, 2017, 36(6):1118-1123.

    LI Y S, CHEN L, GUO Q, et al. Pollution characteristics of phthalate esters in greenhouse agricultural soil in Xinmin, Shenyang City[J]. Journal of Agro-Environment Science, 2017, 36(6):1118-1123(in Chinese).

    [30] 郑顺安, 薛颖昊, 李晓华, 等. 山东寿光设施菜地土壤-农产品邻苯二甲酸酯(PAEs)污染特征调查[J]. 农业环境科学学报, 2016, 35(3):492-499.

    ZHENG S A, XUE Y H, LI X H, et al. Phthalate acid esters(PAEs)pollution in soils and agricultural products of vegetable greenhouses in Shouguang City, Shandong Province[J]. Journal of Agro-Environment Science, 2016, 35(3):492-499(in Chinese).

    [31] 韩蕊, 王冬莹, 芮洋, 等. 一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究[J]. 环境科学学报, 2013, 33(11):2941-2946.

    HAN R, WANG D Y, RUI Y, et al. Biodegradation of PAEs by a newly isolated fungus eupenicillum javanicun F9.[J]. Acta Scientiae Circumstantiae, 2013, 33(11):2941-2946(in Chinese).

    [32] 李晓军, 李培军, 蔺昕. 土壤中难降解有机污染物锁定机理研究进展[J]. 应用生态学报, 2007, 18(7):1624-1630.

    LI X J, LI P J, LIN X. Reserch advances in sequestration mechanisms of hardly biodegradable organic contaminants in soil[J]. Chinese Journal of Applied Ecology, 2007, 18(7):1624-1630(in Chinese).

  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2174
  • HTML全文浏览数:  2174
  • PDF下载数:  42
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-01-04
  • 刊出日期:  2019-12-10
梁浩花, 王亚娟, 陶红, 张小红. 一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
引用本文: 梁浩花, 王亚娟, 陶红, 张小红.

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu
LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong. Isolation, identification and degradation characteristics of a three phthalates degrading bacterium[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
Citation: LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong.

Isolation, identification and degradation characteristics of a three phthalates degrading bacterium

[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

    通讯作者: 王亚娟, E-mail: 358437013@qq.com ; 
  • 1. 宁夏大学资源环境学院, 银川, 750021;
  • 2. 宁夏大学经济管理学院, 银川, 750021;
  • 3. 宁夏(中阿)旱区资源评价与环境调控重点实验室, 银川, 750021
  • 收稿日期:  2019-01-04
  • 网络出版日期:  2019-12-10
基金项目:

宁夏回族自治区重点研发计划项目（2016KJHM29）资助.

摘要: 

选择邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二丁酯（DnBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）作为目标污染物，采用富集驯化法从设施菜地土壤中筛选出1株可同时降解DMP、DnBP和DEHP的细菌MB1.经形态、生理生化特征及16S rDNA序列分析，初步鉴定为微杆菌属（Microbacterium sp.）.通过正交试验研究了该菌株的最优降解条件以及最优条件下该菌株的生长曲线和降解曲线，最后在培养条件下研究了该菌株对人工污染土壤中邻苯二甲酸酯的降解特性.结果表明，菌株MB1的最优降解条件为：pH值为8，温度为25℃，接菌量为5%，每种邻苯二甲酸酯浓度为300 mg·L-1.在此最优条件下该菌株呈S型曲线增长，7 d后无机盐培养液中DMP、DnBP和DEHP的降解率分别为99.62%%、99.65%和55.26%.人工污染土壤中空白试验和投加菌株试验结果为：在不添加菌液的处理中，灭菌土壤21 d时DMP、DnBP和DEHP的降解率分别为3.86%、4.19%和2.01%；未灭菌土壤21 d时对DMP、DnBP和DEHP的降解率分别为4.82%、5.99%和3.44%.在添加菌液的处理中，21 d时土壤灭菌处理中DMP、DnBP和DEHP的降解率分别达94.45%、95.65%和39.21%；而土壤未灭菌处理中DMP、DnBP和DEHP的降解率分别达94.93%、95.99%和41.16%.该结果表明：土壤中土著微生物仅能降解微量PAEs，菌株MB1对土壤中DMP、DnBP和DEHP等3种PAEs污染物具有较为高效的降解能力，未灭菌土壤中邻苯二甲酸酯的降解效果略高于灭菌土壤.

English Abstract

    全文HTML

参考文献 (32)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心