高级搜索

金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制

,  ,  , 

downloadPDF

上一篇

下一篇

阮珍, 刘永军, 刘静, 刘磐. 金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制[J]. 环境化学, 2019, (12): 2649-2656. doi: 10.7524/j.issn.0254-6108.2019011603
引用本文: 阮珍, 刘永军, 刘静, 刘磐.

金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制

[J]. 环境化学, 2019, (12): 2649-2656. doi: 10.7524/j.issn.0254-6108.2019011603
shu
RUAN Zhen, LIU Yongjun, LIU Jing, LIU Pan. Effect of metal ions on the degradation of pyrene by Rhodococcus ruber L9 and its mechanism of action[J]. Environmental Chemistry, 2019, (12): 2649-2656. doi: 10.7524/j.issn.0254-6108.2019011603
Citation: RUAN Zhen, LIU Yongjun, LIU Jing, LIU Pan.

Effect of metal ions on the degradation of pyrene by Rhodococcus ruber L9 and its mechanism of action

[J]. Environmental Chemistry, 2019, (12): 2649-2656. doi: 10.7524/j.issn.0254-6108.2019011603
shu

金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制

  • 西安建筑科技大学 环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安, 710055

    • 通讯作者: 刘永军, E-mail: liuyongjun@xauat.edu.cn ; 
  • 基金项目:

    国家自然科学基金（51178377）资助.

Effect of metal ions on the degradation of pyrene by Rhodococcus ruber L9 and its mechanism of action

  • Shaanxi Key Lab of Environmental Engineering, Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an, 710055, China

    • Corresponding author: LIU Yongjun, liuyongjun@xauat.edu.cn ; 
  • Fund Project: Supported by the National Natural Science Foundation of China (51178377).

  • 摘要:

    实验室从石油污染土壤中筛选得到一株高效芘降解菌，命名为赤红球菌（RhodococcusruberL9），分析了不同浓度Fe3+、Ca2+、Cu2+、Mn2+对该菌降解芘效果的影响.结果发现，0.45 mmol·L-1 Fe3+对芘的降解率有明显促进作用，6d时芘降解率最高，可达77%，相较于不加金属离子时提升了约30%.进一步研究发现，当Fe3+存在时，芘降解过程中蛋白总量变化、邻苯二酚1，2-双加氧酶（C120）和邻苯二酚2，3-双加氧酶（C230）酶活性的变化与Fe3+和Fe2+在胞内、胞外浓度的变化密切相关，主要原因是Fe3+的存在，能诱导赤红球菌合成更多与芘降解有关的蛋白，且可以作为酶的活性中心提高酶的活性，从而大幅提高芘的降解效率.

    Abstract:

    High-efficiency pyrene-degrading bacteria, Rhodococcus ruberL9, was isolated from petroleum contaminated soil. The degradation effect of four metal ions (Fe3+, Ca2+, Cu2+, and Mn2+) on pyrene was investigated in this study. The results showed that 0.45 mmol·L-1 Fe3+ considerably enhanced the degradation efficiency of pyrene. The degradation rate of pyrene reached a maximum of 77% on day 6, which was about 30% higher than that of the control group. Furthermore, we found that when Fe3+ was present during the degradation process, changes in the activity of catechol 1,2-dioxygenase (C120) and catechol 2,3-dioxygenase (C230) and the concentrations of protein were strictly related to changes in intracellular and extracellular Fe3+ and Fe2+ concentrations. This is because the presence of Fe3+ induced Rhodococcus ruberL9 to synthesize large quantities of catechol 1,2-dioxygenase and functioned as an active site in the enzyme to increase enzyme activity, thereby drastically improving the degradation efficiency of pyrene.

  • 加载中
  • [1] ROYCHOWDHURY S, MITRA D. Separation of polyaromatic hydrocarbons from model diesel composition via pervaporation using a fabricated aromatic polyimide membrane and process optimization[J]. Environmental Progress & Sustainable Energy, 2018,10(1):1028-1030.
    [2] KADRI T, ROUISSI T, BRAR S K, et al. Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes:A review[J]. Journal of Environmental Sciences, 2017, 29(1):52-74.
    [3] 李婧, 党志, 郭楚玲,等. 复合固定化法固定微生物去除芘[J]. 环境化学, 2012, 31(7):1036-1042.

    LI J, DANG Z, GUO C L, et al. Removal of pyrene using immobilized microorganism[J]. Environmental Chemistry,2012,31(7):1036-1042(in Chinese).

    [4] 李想,张雪英,周俊,等.1株铜绿假单胞菌对芘的降解特性及代谢途径[J].环境科学, 2018,39(4):1794-1803.

    LI X, ZHANG X Y, ZHOU J,et al. Degradation characteristics and metabolic pathway of a pyrene-degrading Pseudomonas aeruginosa Strain[J]. Environmental Science, 2018,39(4):1794-1803.

    [5] HARITASH A K, KAUSHIK C P. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs):A review[J]. Journal of Hazardous Materials, 2009, 169(1):1-15.
    [6] 王涛, 蓝慧, 田云, 等. 多环芳烃的微生物降解机制研究进展[J]. 化学与生物工程, 2016, 33(2):8-14.

    WANG T, LAN H, TIAN Y, et al. Research prog ress on microbial degradation mechanisms for poly cyclic aromatic hydrocarbons[J].Chemistry & Bioengineering, 2016, 33(2):8-14(in Chinese).

    [7] 苏梦缘, 王红旗, 李艺,等.多环芳烃降解菌菌群构建及其适宜降解环境条件的确定[J]. 环境工程学报, 2017, 11(2):1192-1198.

    SU M Y, WANG H Q, LI Y, et al. Construction of microbial consortia for PAHs degradation and appropriate environmental condition for degradation[J].Chinese Journal of Environmental Engineering, 2017, 11(2):1192-1198(in Chinese).

    [8] 陈政阳, 刘国, 唐彬彬,等. 油基泥浆钻井岩屑中高效石油降解菌的筛选及其降解特性[J]. 环境工程, 2018, 36(2):48-53.

    CHEN Z Y, LIU G, TANG B B, et al. Isolation of degrading bacteria from oil base mud drilling cuttings and its degrading characteristics[J]. Environmental Engineering, 2018, 36(2):48-53(in Chinese).

    [9] 陈亚婷,姜博,邢奕,等.基于非培养手段的多环芳烃降解微生物解析[J]. 中国环境科学, 2018,38(9):3562-3575.

    CHEN Y T, JIANG B, XIE Y, et al.Identification and characterization on PAHs-degrading microorganisms via cultivation-independent approaches[J]. China Environmental Science, 2018,38(9):3562-3575(in Chinese).

    [10] 刁硕,王红旗,许洁,等.低温耐盐芘降解菌的筛选鉴定及降解特性研究[J].中国环境科学,2017,37(2):677-685.

    DIAO S, WANG H Q, XU J, et al.Isolation, identification and analysis of degradation ability of a cold-resistant haloduric pyrene-degrading strains[J]. China Environmental Science,2017,37(2):677-685(in Chinese).

    [11] 侯晓鹏, 李春华, 叶春, 等.不同电子受体作用下微生物降解多环芳烃研究进展[J].环境工程技术学报, 2016, 6(1):78-84.

    HOU X P, LI C H, YE C, et al.Research progress of biodegradation of polycyclic aromatic hydrocarbons with amendment of different electron acceptors[J]. Journal of Environmental Engineering Technology, 2016, 6(1):78-84(in Chinese).

    [12] 吴蔓莉, 聂麦茜, 王晓昌,等.金属离子对多环芳烃酶促降解的影响作用[J]. 水处理技术, 2008, 34(8):13-16.

    WU M L, NIE M Q, WANG X C, et al. Effect of metal ions on enzymatic degradation of polycyclic aromatic hydrocarbons[J]. Technology of Water Treatment, 2008, 34(8):13-16(in Chinese).

    [13] 徐苗, 晁群芳. 一株产胞外邻苯二酚-2,3-双加氧酶菌种的筛选及鉴定[J]. 中国农业科技导报, 2017, 19(12):34-42.

    XU M, ZHAO Q F. Study on the synthesis and characteristic of hexadecylldimethyl (2-sulphite) ethyl ammonium[J]. Technology of Water Treatment, 2017, 19(12):34-42(in Chinese).

    [14] 邵猛猛. 多环芳烃降解菌的筛选、降解性能及环境安全影响研究[D].西安:西安建筑科技大学,2018. SHAO M M.Isolation of PAHs-degrading bacteria, study on their characterization of the degradation metabolism and environment safety impact[D]. XI'an:Xi'an University of Architecture and Technology.2018(in Chinese).
    [15] 杨宏伟, 吴亚丽,马旭阳, 等.黄河干流表层沉积物铁形态的分布特征及相关性分析[J]. 中国环境科学, 2015,35(12):3663-3669.

    YANG H W, WU Y L, MA X Y, et al. Distribution characteristics and correlation analysis of Fe speciation in the surface sediments from different reaches along Yellow River.[J].China Environmental Science, 2015,35(12):3663-3669(in Chinese).

    [16] 邓梦, 杨正凤, 黄遵锡, 等.粪便微生物宏基因组来源的热稳定性邻苯二酚1,2-双加氧酶异源表达及酶学性质[J]. 微生物学通报, 2017, 44(8):1947-1957.

    DENG M, YANG Z F, HUANG Z X, et al. Expression and characterization of thermostable catechol 1,2-dioxygenase from a fecal microbial metagenome of Nycticebuspygmaeus[J]. Microbiol. China, 2017, 44(8):1947-1957(in Chinese).

    [17] 卫昆, 陈烁娜, 尹华, 等. 蜡状芽胞杆菌对芘的降解特性及降解酶研究[J]. 环境科学学报, 2016, 36(2):506-512.

    WEI K, CHEN S N, YIN H, et al. The biodegradation characteristics of pyrene bacillus cereus and its enzyme[J]. Acta Scientiae Circumstantiae, 2016, 36(2):506-512(in Chinese).

    [18] 孔德康,李艺,王红旗,等红球菌跨膜运输荧蒽的差异膜蛋白分析[J]. 中国环境科学, 2019,39(1):274-280

    KONG D K, LI Y, WANG H Q, et al.Differential membrane protein analysis of Rhodococcus during the transmembrane-transport process of fluoranthene[J].China Environmental Science, 2019,39(1):274-280

    [19] SENDA T, SUGIYAMA K, NARITA H, et al. Three-dimensional structures of free form and two substrate complexes of an extradiol ring-cleavage type dioxygenase, the BphC enzyme from Pseudomonas sp. strain KKS102[J]. Journal of Molecular Biology, 1996, 255(5):735-752.
    [20] 周鑫淼, 陈洁君, 耿立召,等.邻苯二酚2,3-双加氧酶的结构和功能研究进展[J]. 生物技术通报, 2007,4(1):51-54.

    ZHOU X M, CHEN J J, GENG L Z, et al. Advances in study on structure and function of catechol 2, 3-Dioxygenase[J]. Biotechnology Bulletin, 2007,4(1):51-54(in Chinese).

  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1844
  • HTML全文浏览数:  1844
  • PDF下载数:  36
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-01-16
  • 刊出日期:  2019-12-10

金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制

    通讯作者: 刘永军, E-mail: liuyongjun@xauat.edu.cn ; 
  • 西安建筑科技大学 环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安, 710055
  • 收稿日期:  2019-01-16
  • 网络出版日期:  2019-12-10
基金项目:

国家自然科学基金（51178377）资助.

摘要: 

实验室从石油污染土壤中筛选得到一株高效芘降解菌，命名为赤红球菌（RhodococcusruberL9），分析了不同浓度Fe3+、Ca2+、Cu2+、Mn2+对该菌降解芘效果的影响.结果发现，0.45 mmol·L-1 Fe3+对芘的降解率有明显促进作用，6d时芘降解率最高，可达77%，相较于不加金属离子时提升了约30%.进一步研究发现，当Fe3+存在时，芘降解过程中蛋白总量变化、邻苯二酚1，2-双加氧酶（C120）和邻苯二酚2，3-双加氧酶（C230）酶活性的变化与Fe3+和Fe2+在胞内、胞外浓度的变化密切相关，主要原因是Fe3+的存在，能诱导赤红球菌合成更多与芘降解有关的蛋白，且可以作为酶的活性中心提高酶的活性，从而大幅提高芘的降解效率.

English Abstract

    全文HTML

参考文献 (20)

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心