高级搜索

全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系

,  , 

downloadPDF

上一篇

下一篇

谢蕾, 章涛, 孙红文. 全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系[J]. 环境化学, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
引用本文: 谢蕾, 章涛, 孙红文. 全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系[J]. 环境化学, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
shu
XIE Lei, ZHANG Tao, SUN Hongwen. Enrichment characteristics of perfluoroalkyl substances(PFASs) in human liver and its association with hepatocyte apoptosis[J]. Environmental Chemistry, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
Citation: XIE Lei, ZHANG Tao, SUN Hongwen. Enrichment characteristics of perfluoroalkyl substances(PFASs) in human liver and its association with hepatocyte apoptosis[J]. Environmental Chemistry, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
shu

全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系

  • 1. 中山大学环境科学与工程学院, 广州, 510275;

  • 2. 南开大学环境科学与工程学院, 天津, 300350

    • 通讯作者: 章涛, E-mail: zhangt47@mail.sysu.edu.cn ; 
  • 基金项目:

    国家自然科学基金（21677184，41877375）资助.

Enrichment characteristics of perfluoroalkyl substances(PFASs) in human liver and its association with hepatocyte apoptosis

  • 1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China;

  • 2. College of Environmental Science and Engineering, NanKai University, Tianjin, 300350, China

    • Corresponding author: ZHANG Tao, zhangt47@mail.sysu.edu.cn ; 
  • Fund Project: Supported by the National Natural Science Foundation of China (21677184, 41877375).

  • 摘要: 全氟烷基化合物（polyfluoroalkyl substances，PFASs，F（CF2）n-R）是广泛用于生产生活中的有机污染物，研究发现PFASs的暴露会对人体造成危害.为分析人体肝脏中PFASs的富集特征，本研究采用高效液相色谱串联质谱（HPLC-MS/MS）的方法，对天津市31例肝癌患者肝脏中10种PFASs（全氟己烷磺酸PFHxS、全氟辛烷磺酸PFOS、全氟十二酸PFDoDA、全氟己酸PFHxA、全氟庚酸PFHpA、全氟十一酸PFUnDA、全氟壬酸PFNA、全氟癸酸PFDA、全氟辛酸PFOA、全氟戊酸PFPeA）进行检测分析.结果表明，PFOS、PFOA、PFNA、PFUnDA和PFHxS具有较高的检出率（>80%）；∑PFASs浓度范围为8.97-181.87 ng·g-1，其中PFOS的平均浓度最高（40.87 ng·g-1），占∑PFASs的77%；∑PFASs的平均浓度在男性肝脏样品中（64.04 ng·g-1）与女性样品中（36.82 ng·g-1）存在显著性差异，PFHxS、PFOS和PFDA的浓度在性别上也均呈显著差异，PFOA的浓度与年龄呈显著负相关性.此外，PFOA和PFHpA的浓度分别与肝细胞损伤标志物ALT和GGT之间存在显著正相关性.本研究是国内首次对人体肝脏中PFASs的暴露分析，PFOS是肝脏的主要暴露物；PFASs对男性的暴露风险高于女性，且青年人群PFASs的暴露风险相对较高；另外，浓度水平进行分析，PFASs的暴露与人体肝脏损伤存在统计学关联.
    Abstract: Perfluoroalkyl substances (PFASs, F(CF2)n-R), a group of persistent organic pollutants, are widely used in industrial and daily life. Several studies have rported that PFASs exposure would have adverse effect to human health. To investigate whether PFASs exposure would increase the risk of human liver disease, concentrations of 10 PFASs (PFHxS, PFOS, PFDoDA, PFHxA, PFHpA, PFUnDA, PFNA, PFDA, PFOA, PFPeA) in liver samples, collected from 31 liver cancer patients in Tianjin city of China, were analyzed by using high performance chromatography-tandem mass spectrometry (HPLC-MS/MS). Higher detection rates (> 80%) were found for PFOS, PFOA, PFNA, PFUnDA and PFHxS. Concentrations of ∑PFASs ranged from 8.97 to 181.87 ng·g-1, with PFOS showing the highest mean level (40.87 ng·g-1) and accounting for 77% of the total PFASs. In addition, levels of ∑PFASs in males (64.04 ng·g-1) were significantly higher (P < 0.05) than those in females (36.82 ng·g-1). Also, gender differences in the concentrations of PFHxS, PFOS and PFDA were also observed. Negative correlations were found between PFOA concentration and age. Moreover, positive correlations were found between PFOA concentration and ALT, PFHpA concentration and GGT, respectively. This study is the first to report the exposure of PFASs in human liver. PFOS was the predominant PFASs in human liver samples. Males and the younger group suffer higher cumulative exposure risk. Furthermore, there was a statistical correlation between PFASs exposure and human liver injury.
  • 加载中
  • [1] WANG Z, DEWITT J C, HIGGINS C P, et al. A Never-ending story of per-and polyfluoroalkyl substances (PFASs)?[J]. Environmental Science & Technology, 2017,51(5):2508-2518.
    [2] NELSON J W, HATCH E E, WEBSTER T F. Exposure to polyfluoroalkyl chemicals and cholesterol, body weight, and insulin resistance in the general U.S. population[J]. Environ Health Perspect, 2010,118(2):197-202.
    [3] JONES P D, HU W, DE COEN W, et al. Binding of perfluorinated fatty acids to serum proteins[J]. Environmental Toxicology and Chemistry, 2003,22(11):2639-2649.
    [4] KARÁSKOVÁ P, VENIER M, MELYMUK L, et al. Perfluorinated alkyl substances (PFASs) in household dust in Central Europe and North America[J]. Environment International, 2016,94:315-324.
    [5] ZHANG T, WU Q, SUN H W, et al. Perfluorinated compounds in whole blood samples from infants, children, and adults in China[J]. Environmental Science & Technology, 2010,44(11):4341-4347.
    [6] ZHENG F, SHENG N, ZHANG H, et al. Perfluorooctanoic acid exposure disturbs glucose metabolism in mouse liver[J]. Toxicology and Applied Pharmacology, 2017,335:41-48.
    [7] MANERA M, GIARI L, VINCENZI F, et al. Texture analysis in liver of common carp (Cyprinus carpio) sub-chronically exposed to perfluorooctanoic acid[J]. Ecological Indicators, 2017,81:54-64.
    [8] LAU C, ANITOLE K, HODES C, et al. Perfluoroalkyl acids:A review of monitoring and toxicological findings[J]. Toxicol Sci, 2007,99(2):366-394.
    [9] ZHANG T, SUN H, LIN Y, et al. Perfluorinated compounds in human blood, water, edible freshwater fish, and seafood in china:Daily intake and regional differences in human exposures[J]. Journal of Agricultural and Food Chemistry, 2011,59(20):11168-11176.
    [10] ZHANG T, SUN H, QIN X, et al. PFOS and PFOA in paired urine and blood from general adults and pregnant women:Assessment of urinary elimination[J]. Environmental Science and Pollution Research International, 2015,22(7):5572-5579.
    [11] ZHANG Y, JIANG W, FANG S, et al. Perfluoroalkyl acids and the isomers of perfluorooctanesulfonate and perfluorooctanoate in the sera of 50 new couples in Tianjin, China[J]. Environment International, 2014,68:185-191.
    [12] VERREAULT J, HOUDE M, GABRIELSEN G W, et al. Perfluorinated alkyl substances in plasma, liver, brain, and eggs of glaucous gulls (larus hyperboreus) from the norwegian arctic[J]. Environmental Science & Technology, 2005,39(19):7439-7445.
    [13] HANSEN K J, CLEMEN L A, ELLEFSON M E, et al. Compound-specific, quantitative characterization of organic fluorochemicals in biological matrices[J]. Environmental Science & Technology, 2001,35(4):766-770.
    [14] OLSEN G W, HANSEN K J, STEVENSON L A, et al. Human donor liver and serum concentrations of perfluorooctanesulfonate and other perfluorochemicals[J]. Environmental Science & Technology, 2003,37(5):888-891.
    [15] MAESTRI L, NEGRI S, FERRARI M, et al. Determination of perfluorooctanoic acid and perfluorooctanesulfonate in human tissues by liquid chromatography/single quadrupole mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2006,20(18):2728-2734.
    [16] KÄRRMAN A, DOMINGO J L, LLEBARIA X, et al. Biomonitoring perfluorinated compounds in Catalonia, Spain:Concentrations and trends in human liver and milk samples[J]. Environmental Science and Pollution Research, 2010,17(3):750-758.
    [17] YEUNG L W Y, GURUGE K S, TANIYASU S, et al. Profiles of perfluoroalkyl substances in the liver and serum of patients with liver cancer and cirrhosis in Australia[J]. Ecotoxicology and Environmental Safety, 2013,96:139-146.
    [18] MAMSEN L S, JÖNSSON B A G, LINDH C H, et al. Concentration of perfluorinated compounds and cotinine in human foetal organs, placenta, and maternal plasma[J]. Science of the Total Environment, 2017,596-597:97-105.
    [19] MAMSEN L S, BJÖRVANG R D, MUCS D, et al. Concentrations of perfluoroalkyl substances (PFASs) in human embryonic and fetal organs from first, second, and third trimester pregnancies[J]. Environment International, 2019,124:482-492.
    [20] LI L, ZHAI Z, LIU J, et al. Estimating industrial and domestic environmental releases of perfluorooctanoic acid and its salts in China from 2004 to 2012[J]. Chemosphere, 2015,129:100-109.
    [21] OLSEN G W, MAIR D C, LANGE C C, et al. Per-and polyfluoroalkyl substances (PFAS) in American Red Cross adult blood donors, 2000-2015[J]. Environmental Research, 2017,157:87-95.
    [22] XIE S, WANG T, LIU S, et al. Industrial source identification and emission estimation of perfluorooctane sulfonate in China[J]. Environment International, 2013,52:1-8.
    [23] JIN Y, SAITO N, HARADA K H, et al. Historical trends in human serum levels of perfluorooctanoate and perfluorooctane sulfonate in Shenyang, China[J]. The Tohoku Journal of Experimental Medicine, 2007,212(1):63-70.
    [24] SUNDERLAND E M, HU X C, DASSUNCAO C, et al. A review of the pathways of human exposure to poly-and perfluoroalkyl substances (PFASs) and present understanding of health effects[J]. Journal of Exposure Science & Environmental Epidemiology, 2019,29(2):131-147.
    [25] NAKAYAMA K, IWATA H, TAO L, et al. Potential effects of perfluorinated compounds in common cormorants from Lake Biwa, Japan:An implication from the hepatic gene expression profiles by microarray[J]. Environmental Toxicology and Chemistry, 2008,27(11):2378.
    [26] GURUGE K S, MANAGE P M, YAMANAKA N, et al. Species-specific concentrations of perfluoroalkyl contaminants in farm and pet animals in Japan[J]. Chemosphere, 2008,73(1):S210-S215.
    [27] ISHIBASHI H, IWATA H, KIM E, et al. Contamination and effects of perfluorochemicals in baikal seal (pusa sibirica).1. Residue level, tissue distribution, and temporal trend[J]. Environmental Science & Technology, 2008,42(7):2295-2301.
    [28] KANNAN K, CORSOLINI S, FALANDYSZ J, et al. Perfluorooctanesulfonate and related fluorinated hydrocarbons in marine mammals, fishes, and birds from coasts of the baltic and the mediterranean seas[J]. Environmental Science & Technology, 2002,36(15):3210-3216.
    [29] CHO C R, LAM N H, CHO B M, et al. Concentration and correlations of perfluoroalkyl substances in whole blood among subjects from three different geographical areas in Korea[J]. Science of The Total Environment, 2015,512-513:397-405.
    [30] FU Y, WANG T, WANG P, et al. Effects of age, gender and region on serum concentrations of perfluorinated compounds in general population of Henan, China[J]. Chemosphere, 2014,110:104-110.
    [31] HSU J, HSU J, HO H, et al. Background levels of persistent organic pollutants in humans from taiwan:Perfluorooctane sulfonate and perfluorooctanoic acid[J]. Chemosphere, 2013,93(3):532-537.
    [32] WAN H T, LEUNG P Y, ZHAO Y G, et al. Blood plasma concentrations of endocrine disrupting chemicals in Hong Kong populations[J]. Journal of Hazardous Materials, 2013,261:763-769.
    [33] FROMME H, MIDASCH O, TWARDELLA D, et al. Occurrence of perfluorinated substances in an adult German population in southern Bavaria[J]. International Archives of Occupational and Environmental Health, 2007,80(4):313-319.
    [34] KÄRRMAN A, MUELLER J F, van BAVEL B, et al. Levels of 12 perfluorinated chemicals in pooled australian serum, collected 2002-2003, in Relation to Age, Gender, and Region[J]. Environmental Science & Technology, 2006,40(12):3742-3748.
    [35] CALAFAT A M, KUKLENYIK Z, REIDY J A, et al. Serum concentrations of 11 polyfluoroalkyl compounds in the U.S. Population:Data from the National Health and Nutrition Examination Survey (NHANES) 1999-2000[J]. Environmental Science & Technology, 2007,41(7):2237-2242.
    [36] WONG F, MACLEOD M, MUELLER J F, et al. Enhanced elimination of perfluorooctane sulfonic acid by menstruating women:Evidence from population-based pharmacokinetic modeling[J]. Environmental Science & Technology, 2014,48(15):8807-8814.
    [37] FROMME H, MOSCH C, MOROVITZ M, et al. Pre-and postnatal exposure to perfluorinated compounds (PFCs)[J]. Environmental Science & Technology, 2010,44(18):7123-7129.
    [38] TAO L, MA J, KUNISUE T, et al. Perfluorinated compounds in human breast milk from several Asian countries, and in infant formula and dairy milk from the United States[J]. Environmental Science & Technology, 2008,42(22):8597-8602.
    [39] HARADA K, INOUE K, MORIKAWA A, et al. Renal clearance of perfluorooctane sulfonate and perfluorooctanoate in humans and their species-specific excretion[J]. Environmental Research, 2005,99(2):253-261.
    [40] HAUG L S, THOMSEN C, BECHER G. Time trends and the influence of age and gender on serum concentrations of perfluorinated compounds in archived human samples[J]. Environmental Science & Technology, 2009,43(6):2131-2136.
    [41] KLEPEIS N E, NELSON W C, OTT W R, et al. The National Human Activity Pattern Survey (NHAPS):A resource for assessing exposure to environmental pollutants[J]. J Expo Anal Environ Epidemiol, 2001,11(3):231-252.
    [42] PAN Y, SHI Y, WANG J, et al. Concentrations of perfluorinated compounds in human blood from twelve cities in China[J]. Environmental Toxicology and Chemistry, 2010,29(12):2695-2701.
    [43] KANNAN K, CORSOLINI S, FALANDYSZ J, et al. Perfluorooctanesulfonate and related fluorochemicals in human blood from several countries[J]. Environmental Science & Technology, 2004,38(17):4489-4495.
    [44] YEUNG L W Y, SO M K, JIANG G, et al. Perfluorooctanesulfonate and related fluorochemicals in human blood samples from China[J]. Environmental Science & Technology, 2006,40(3):715-720.
    [45] JAIN R B, Ducatman A. Selective associations of recent low concentrations of perfluoroalkyl substances with liver function biomarkers:NHANES 2011-2014 Data on US Adults Aged ≥ 20 Years[J]. Journal of Occupational and Environmental Medicine, 2019, 61(4):293-302.
    [46] DARROW L A, GROTH A C, WINQUIST A, et al. Modeled perfluorooctanoic acid (PFOA) exposure and liver function in a Mid-Ohio Valley Community[J]. Environmental Health Perspectives, 2016,124(8):1227-1233.
    [47] GALLO V, LEONARDI G, GENSER B, et al. Serum perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) concentrations and liver function biomarkers in a population with elevated PFOA Exposure[J]. Environmental Health Perspectives, 2012,120(5):655-660.
    [48] BASSLER J, DUCATMAN A, ELLIOTT M, et al. Environmental perfluoroalkyl acid exposures are associated with liver disease characterized by apoptosis and altered serum adipocytokines[J]. Environmental Pollution, 2019,247:1055-1063.
    [49] XIE Y, YANG Q, NELSON B D, et al. The relationship between liver peroxisome proliferation and adipose tissue atrophy induced by peroxisome proliferator exposure and withdrawal in mice[J]. Biochemical Pharmacology, 2003,66(5):749-756.
    [50] PHILIPPE G, ELISABETH WREFORD A, ESBEN B, et al. Serum vaccine antibody concentrations in children exposed to perfluorinated compounds[J]. Journal of the American Medical Association, 2012,307(4):391-397.
    [51] WANG J, ZENG X, BLOOM M S, et al. Renal function and isomers of perfluorooctanoate (PFOA) and perfluorooctanesulfonate (PFOS):Isomers of C8 Health Project in China[J]. Chemosphere, 2019,218:1042-1049.
    [52] SHANKAR A, XIAO J, DUCATMAN A. Perfluoroalkyl chemicals and chronic kidney disease in US Adults[J]. American Journal of Epidemiology, 2011,174(8):893-900.
    [53] TIAN Y, ZENG X, BLOOM M S, et al. Isomers of perfluoroalkyl substances and overweight status among Chinese by sex status:Isomers of C8 Health Project in China[J]. Environment International, 2019,124:130-138.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  4320
  • HTML全文浏览数:  4320
  • PDF下载数:  137
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-04-11
谢蕾, 章涛, 孙红文. 全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系[J]. 环境化学, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
引用本文: 谢蕾, 章涛, 孙红文. 全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系[J]. 环境化学, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
shu
XIE Lei, ZHANG Tao, SUN Hongwen. Enrichment characteristics of perfluoroalkyl substances(PFASs) in human liver and its association with hepatocyte apoptosis[J]. Environmental Chemistry, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
Citation: XIE Lei, ZHANG Tao, SUN Hongwen. Enrichment characteristics of perfluoroalkyl substances(PFASs) in human liver and its association with hepatocyte apoptosis[J]. Environmental Chemistry, 2020, (6): 1479-1487. doi: 10.7524/j.issn.0254-6108.2019041103
shu

全氟烷基化合物在人体肝脏中的富集特征及其与肝损伤的关系

    通讯作者: 章涛, E-mail: zhangt47@mail.sysu.edu.cn ; 
  • 1. 中山大学环境科学与工程学院, 广州, 510275;
  • 2. 南开大学环境科学与工程学院, 天津, 300350
  • 收稿日期:  2019-04-11
基金项目:

国家自然科学基金（21677184，41877375）资助.

摘要: 全氟烷基化合物（polyfluoroalkyl substances，PFASs，F（CF2）n-R）是广泛用于生产生活中的有机污染物，研究发现PFASs的暴露会对人体造成危害.为分析人体肝脏中PFASs的富集特征，本研究采用高效液相色谱串联质谱（HPLC-MS/MS）的方法，对天津市31例肝癌患者肝脏中10种PFASs（全氟己烷磺酸PFHxS、全氟辛烷磺酸PFOS、全氟十二酸PFDoDA、全氟己酸PFHxA、全氟庚酸PFHpA、全氟十一酸PFUnDA、全氟壬酸PFNA、全氟癸酸PFDA、全氟辛酸PFOA、全氟戊酸PFPeA）进行检测分析.结果表明，PFOS、PFOA、PFNA、PFUnDA和PFHxS具有较高的检出率（>80%）；∑PFASs浓度范围为8.97-181.87 ng·g-1，其中PFOS的平均浓度最高（40.87 ng·g-1），占∑PFASs的77%；∑PFASs的平均浓度在男性肝脏样品中（64.04 ng·g-1）与女性样品中（36.82 ng·g-1）存在显著性差异，PFHxS、PFOS和PFDA的浓度在性别上也均呈显著差异，PFOA的浓度与年龄呈显著负相关性.此外，PFOA和PFHpA的浓度分别与肝细胞损伤标志物ALT和GGT之间存在显著正相关性.本研究是国内首次对人体肝脏中PFASs的暴露分析，PFOS是肝脏的主要暴露物；PFASs对男性的暴露风险高于女性，且青年人群PFASs的暴露风险相对较高；另外，浓度水平进行分析，PFASs的暴露与人体肝脏损伤存在统计学关联.

English Abstract

    全文HTML

参考文献 (53)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心