蓝藻发酵液中氮磷回收及其作为反硝化碳源研究
Recovery of nitrogen and phosphorus from fermentation liquid of cyanobacteria and its application as a carbon source for denitrification
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摘要: 蓝藻的处理处置及其资源化利用成为亟需解决的问题.本文采用鸟粪石+磷酸钙沉淀法组合工艺回收蓝藻厌氧发酵产酸液中的氮和磷，纯化后的蓝藻发酵液作为外源碳源进行污水的反硝化脱氮利用.实验结果表明，蓝藻通过厌氧发酵可产生大量的挥发性脂肪酸（VFAs），浓度高达25960 mg·L-1，其主要成分乙酸和丁酸的浓度分别为12800 mg·L-1和7062 mg·L-1，占VFAs的76.51%.鸟粪石沉淀的最佳工艺参数为：pH值为9，反应时间为60 min，Mg：P：N的摩尔比为1.2：1.1：1；磷酸钙沉淀的最佳工艺参数为：pH值为10，反应时间为15 min，Ca：P的摩尔比为6.68：1.在最佳条件下，组合工艺处理后的蓝藻发酵液出水NH4+-N和PO43--P浓度为22.83 mg·L-1和2.7 mg·L-1，回收率分别为98.84%和94.62%.反硝化脱氮实验中，蓝藻发酵液作为外源碳源时，NO3--N的去除率达到99.13%，反硝化速率为1.98 mg·（g·h）-1（以MLSS计），反硝化效果比商业碳源（乙醇、乙酸钠）更好，可以作为城市污水处理厂反硝化脱氮的替代碳源.本文可为蓝藻的全流程处理提供借鉴.Abstract: The treatment of cyanobacteria and its resource utilization had become an urgent problem. In this paper, struvite precipitation combined with calcium phosphate precipitation was used to recover ammonia nitrogen and phosphorus in the cyanobacteria acid anaerobic fermentation liquid, the purified cyanobacteria fermentation liquid was used as an exogenous carbon source for wastewater denitrification. The experimental results indicated that a large amount of volatile fatty acids (VFAs) was generated by the anaerobic fermentation from cyanobacteria, with the concentration of 25960 mg·L-1, the main component of acetic acid and butyric acid were 12800 and 7062 mg·L-1, respectively, accounting for 76.51% of VFAs. The optimum process parameters of struvite precipitation were that: the pH value was 9, the reaction time was 60 min, the molar ratio of Mg:P:N was 1.2:1.1:1; the optimal parameters of calcium phosphate precipitation were that: the pH value was 10, the reaction time was 15 min, and the molar ratio of Ca:P was 6.68:1. Under the optimal conditions, the effluent NH4+-N and PO43--P concentration of the cyanobacteria fermentation liquid were 22.83 mg·L-1 and 2.7 mg·L-1, respectively, and the recovery rates were 98.84% and 94.62%, respectively. In the denitrification experiment, when the cyanobacteria fermentation liquid was used as the exogenous carbon source, the removal rate of NO3--N reached 99.13%, and the denitrification rate was 1.98 mg·(g·h)-1 (calculated by MLSS), the denitrification efficiency of the cyanobacteria fermentation liquid was better than the traditional commercial carbon source (ethanol, sodium acetate), and could be used as an alternative carbon source for denitrification in municipal wastewater treatment plants. This paper could provide a reference for the whole process treatment of cyanobacteria.
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