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太湖贝类中微囊藻毒素的测定与健康风险评估

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郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
引用本文: 郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
shu
GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
Citation: GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
shu

太湖贝类中微囊藻毒素的测定与健康风险评估

  • 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京, 210023

    • 通讯作者: 鲜啟鸣, E-mail: xianqm@nju.edu.cn
  • 基金项目:

    太湖水环境综合治理科研课题基金（TH2016301）和"十三五"水专项课题（2017ZX07204004）资助.

Determination and health risk assessment of microcystins in shellfish from Lake Taihu

  • State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China

    • Corresponding author: XIAN Qiming, xianqm@nju.edu.cn
  • Fund Project: Supported by the Taihu Pollution Control Office of Jiangsu Province (TH 2016301) and the Major Science and Technology Program for Water Pollution Control and Treatment (2017ZX07204004).

  • 摘要: 本文调查了太湖环境水体贝类中5种微囊藻毒素microcystins（MCs：MC-RR、MC-YR、MC-LR、MC-LF、MC-LW）的残留水平及其健康风险.采集2017年太湖湖区（梅梁湖和南太湖）的贝类样品（河蚌、河蚬），取可食用组织，经冻干处理后，采用80%甲醇/水溶液涡旋振荡提取，高速离心后的上清液通过HLB固相萃取柱富集净化，采用高效液相色谱-串联质谱法测定样品中MCs的含量.结果表明，MCs的质量浓度在5.00—100.0 μg·L-1范围内呈良好线性关系，相关系数r2均大于0.99.MCs在空白贝类组织中的加标回收率范围为72.4%—82.6%，相对标准偏差（RSD）均小于8%；在太湖贝类可食用组织中都能检测到MC-RR和MC-LR两种常见的MCs，河蚌中藻毒素浓度大于河蚬中藻毒素的浓度，梅梁湖河蚌中总藻毒素含量为0.877 mg·kg-1 dw（干重），高于南太湖河蚌中藻毒素的浓度0.690 mg·kg-1 dw.通过人体健康风险评价模型分析，太湖湖区的贝类存在不同程度的健康风险，其中采集的河蚬样品的风险指数较低，而河蚌样品健康风险指数均大于0.7，接近风险阈值，人类食用河蚌水产品具有一定的潜在健康风险.
    Abstract: This paper investigated the contamination levels and health risks of five microcystins (MCs: MC-RR, MC-YR, MC-LR, MC-LF, MC-LW) in shellfish from Taihu Lake. The shellfish samples were collected in the Taihu Lake (Meiliang Lake and Southern Taihu Lake) in 2017, and the edible tissues of the shellfish were obtained and lyophilized. MCs were extracted with 80% methanol/water solution from a vortex. The supernatant after high-speed centrifugation was enriched and purified by HLB solid phase extraction column, and the content of MCs in the sample was determined by high performance liquid chromatography-tandem mass spectrometry. The results showed that the mass concentration of MCs showed a good linear relationship in the range of 5.00—100.0 μg·L-1, and the correlation coefficient (R2) was greater than 0.99. The spiked recoveries of MCs in blank shellfish tissues ranged from 72.4% to 82.6%, and the relative standard deviation (RSD) values were less than 8%. MC-RR and MC-LR were detected with a relatively high levels in the edible tissues of shellfish from Taihu Lake. The concentration of MCs in Hyriopsis cumingii was higher than that in Corbicula fluminea. The concentrations of MCs in H. cumingii were 0.877 mg·kg-1 dw (dry weight) in Meiliang Lake and 0.690 mg·kg-1 dw in Southern Taihu Lake. Based on the analysis of human health risk assessment models, shellfish in the Taihu Lake region had different degrees of health risks. The risk index of collected C. fluminea samples was low, and the health risk index of H. cumingii samples was greater than 0.7, which was close to the risk threshold.
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  • 收稿日期:  2019-12-08
郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
引用本文: 郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
shu
GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
Citation: GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
shu

太湖贝类中微囊藻毒素的测定与健康风险评估

    通讯作者: 鲜啟鸣, E-mail: xianqm@nju.edu.cn
  • 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京, 210023
  • 收稿日期:  2019-12-08
基金项目:

太湖水环境综合治理科研课题基金（TH2016301）和"十三五"水专项课题（2017ZX07204004）资助.

摘要: 本文调查了太湖环境水体贝类中5种微囊藻毒素microcystins（MCs：MC-RR、MC-YR、MC-LR、MC-LF、MC-LW）的残留水平及其健康风险.采集2017年太湖湖区（梅梁湖和南太湖）的贝类样品（河蚌、河蚬），取可食用组织，经冻干处理后，采用80%甲醇/水溶液涡旋振荡提取，高速离心后的上清液通过HLB固相萃取柱富集净化，采用高效液相色谱-串联质谱法测定样品中MCs的含量.结果表明，MCs的质量浓度在5.00—100.0 μg·L-1范围内呈良好线性关系，相关系数r2均大于0.99.MCs在空白贝类组织中的加标回收率范围为72.4%—82.6%，相对标准偏差（RSD）均小于8%；在太湖贝类可食用组织中都能检测到MC-RR和MC-LR两种常见的MCs，河蚌中藻毒素浓度大于河蚬中藻毒素的浓度，梅梁湖河蚌中总藻毒素含量为0.877 mg·kg-1 dw（干重），高于南太湖河蚌中藻毒素的浓度0.690 mg·kg-1 dw.通过人体健康风险评价模型分析，太湖湖区的贝类存在不同程度的健康风险，其中采集的河蚬样品的风险指数较低，而河蚌样品健康风险指数均大于0.7，接近风险阈值，人类食用河蚌水产品具有一定的潜在健康风险.

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