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太湖贝类中微囊藻毒素的测定与健康风险评估

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郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
引用本文: 郭雅欣, 钱宗耀, 龚婷婷, 鲜啟鸣. 太湖贝类中微囊藻毒素的测定与健康风险评估[J]. 环境化学, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
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GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
Citation: GUO Yaxin, QIAN Zongyao, GONG Tingting, XIAN Qiming. Determination and health risk assessment of microcystins in shellfish from Lake Taihu[J]. Environmental Chemistry, 2020, (10): 2673-2682. doi: 10.7524/j.issn.0254-6108.2019120802
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太湖贝类中微囊藻毒素的测定与健康风险评估

  • 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京, 210023

    • 通讯作者: 鲜啟鸣, E-mail: xianqm@nju.edu.cn
  • 基金项目:

    太湖水环境综合治理科研课题基金（TH2016301）和"十三五"水专项课题（2017ZX07204004）资助.

Determination and health risk assessment of microcystins in shellfish from Lake Taihu

  • State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China

    • Corresponding author: XIAN Qiming, xianqm@nju.edu.cn
  • Fund Project: Supported by the Taihu Pollution Control Office of Jiangsu Province (TH 2016301) and the Major Science and Technology Program for Water Pollution Control and Treatment (2017ZX07204004).

  • 摘要: 本文调查了太湖环境水体贝类中5种微囊藻毒素microcystins（MCs：MC-RR、MC-YR、MC-LR、MC-LF、MC-LW）的残留水平及其健康风险.采集2017年太湖湖区（梅梁湖和南太湖）的贝类样品（河蚌、河蚬），取可食用组织，经冻干处理后，采用80%甲醇/水溶液涡旋振荡提取，高速离心后的上清液通过HLB固相萃取柱富集净化，采用高效液相色谱-串联质谱法测定样品中MCs的含量.结果表明，MCs的质量浓度在5.00—100.0 μg·L-1范围内呈良好线性关系，相关系数r2均大于0.99.MCs在空白贝类组织中的加标回收率范围为72.4%—82.6%，相对标准偏差（RSD）均小于8%；在太湖贝类可食用组织中都能检测到MC-RR和MC-LR两种常见的MCs，河蚌中藻毒素浓度大于河蚬中藻毒素的浓度，梅梁湖河蚌中总藻毒素含量为0.877 mg·kg-1 dw（干重），高于南太湖河蚌中藻毒素的浓度0.690 mg·kg-1 dw.通过人体健康风险评价模型分析，太湖湖区的贝类存在不同程度的健康风险，其中采集的河蚬样品的风险指数较低，而河蚌样品健康风险指数均大于0.7，接近风险阈值，人类食用河蚌水产品具有一定的潜在健康风险.
    Abstract: This paper investigated the contamination levels and health risks of five microcystins (MCs: MC-RR, MC-YR, MC-LR, MC-LF, MC-LW) in shellfish from Taihu Lake. The shellfish samples were collected in the Taihu Lake (Meiliang Lake and Southern Taihu Lake) in 2017, and the edible tissues of the shellfish were obtained and lyophilized. MCs were extracted with 80% methanol/water solution from a vortex. The supernatant after high-speed centrifugation was enriched and purified by HLB solid phase extraction column, and the content of MCs in the sample was determined by high performance liquid chromatography-tandem mass spectrometry. The results showed that the mass concentration of MCs showed a good linear relationship in the range of 5.00—100.0 μg·L-1, and the correlation coefficient (R2) was greater than 0.99. The spiked recoveries of MCs in blank shellfish tissues ranged from 72.4% to 82.6%, and the relative standard deviation (RSD) values were less than 8%. MC-RR and MC-LR were detected with a relatively high levels in the edible tissues of shellfish from Taihu Lake. The concentration of MCs in Hyriopsis cumingii was higher than that in Corbicula fluminea. The concentrations of MCs in H. cumingii were 0.877 mg·kg-1 dw (dry weight) in Meiliang Lake and 0.690 mg·kg-1 dw in Southern Taihu Lake. Based on the analysis of human health risk assessment models, shellfish in the Taihu Lake region had different degrees of health risks. The risk index of collected C. fluminea samples was low, and the health risk index of H. cumingii samples was greater than 0.7, which was close to the risk threshold.
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  • [1] 谢平. 蓝藻水华及其次生危害[J]. 水生态学杂志, 2015, 36(4):1-13.

    XIE P. Cyanobacterial blooms and their secondary harms[J]. Journal of Hydroecology, 2015, 36(4):1-13(in Chinese).

    [2] VAN APELDOORN M E, VAN EGMOND H P, SPEIJERS G J, et al. Toxins of cyanobacteria[J]. Molecular Nutrition & Food Research, 2007, 51(1):7-60.
    [3] WHO. Guidelines for drinking water quality[M]. Third edition. Geneva:World Health Organization, 2004:407-408.
    [4] 中华人民共和国卫生部, 中国国家标准化管理委员会. GB 5749-2006中华人民共和国国家标准-生活饮用水卫生标准[S]. 北京:中国标准出版社, 2006. Ministry of Health of the People's Republic of China, China National Standardization Administration Committee. GB 5749-2006 National Standard of the People's Republic of China-Sanitary Standards for Drinking Water[S]. Beijing:China Standard Press, 2006

    (in Chinese).

    [5] CHORUS I. Toxic cyanobacteria in water-A guide to their public health consequences, monitoring and management[M]. London:World Health Organization, 1999:407-408.
    [6] NISHIWAKI R, OHTA T, SUEOKA E, et al. Two significant aspects of microcystin-LR:Specific binding and liver specificity[J]. Cancer Letters, 1994, 83(1/2):283-289.
    [7] 曹莹, 张亚辉, 高富, 等. 太湖水中微囊藻毒素的测定及其分布特征[J]. 环境科学与技术, 2012, 35(S1):229-233.

    CAO Y, ZHANG Y H, GAO F, et al. Determination and the distribution characteristics of microcystins in the water of Taihu Lake[J]. Environmental Science & Technology, 2012, 35(S1):229-233(in Chinese).

    [8] 姜锦林, 周军英, 刘仁彬, 等. 太湖重污染湖区和水源地水质概况及藻毒素污染环境风险[J]. 生态毒理学报, 2019, 14(3):60-71.

    JIANG J L, ZHOU J Y, LIU R B, et al. General situation of water quality and environmental risks of algal toxins pollution in major polluted area and water sources of Lake Taihu[J]. Asian Journal of Ecotoxicology, 2019, 14(3):60-71(in Chinese).

    [9] MCELHINEY J, LAWTON L A. Detection of the cyanobacterial hepatotoxins microcystins[J]. Toxicology & Applied Pharmacology, 2005, 203(3):219-230.
    [10] 张维昊, 徐小清. 固相萃取高效液相色谱法测定水中痕量-微囊藻毒素[J]. 分析化学, 2001, 29(5):522-525.

    ZHANG W H, XU X Q. Determination of trace level microcystins in water using solid-phase extraction and high performance liquid chromatography[J]. Chinese Journal of Analytical Chemistry, 2001, 29(5):522-525(in Chinese).

    [11] 郭坚, 杨新磊, 叶明立. 全自动在线固相萃取-高效液相色谱法测定水体中痕量微囊藻毒素[J]. 分析化学, 2011, 39(8):1256-1260.

    GUO J, YANG X L, YE M L. Determination of trace level microcystins in water by fully automated online solid phase extraction coupled with high performance liquid chromatography[J]. Chinese Journal of Analytical Chemistry, 2011, 39(8):1256-1260(in Chinese).

    [12] FOSS A J, AUBEL M T. Using the MMPB technique to confirm microcystin concentrations in water measured by ELISA and HPLC (UV, MS, MS/MS)[J]. Toxicon Official Journal of the International Society on Toxinology, 2015, 104:91-101.
    [13] 顾丽丽. ELISA试剂盒法测定水中LR型微囊藻毒素[J]. 化学分析计量, 2013, 22(1):97-99.

    GU L L. Determination of microcystin-LR in water by ELISA kit[J]. Chemical Analysis and Meterage 2013, 22(1):97-99(in Chinese).

    [14] 吴幸强, 龚艳, 王智, 等. 微囊藻毒素在滇池鱼体内的积累水平及分布特征[J]. 水生生物学报, 2010, 34(2):388-393.

    WU X Q, GONG Y, WANG Z, et al. Residue levels and distribution features of microcystins in fish samples from Lake Dianchi[J]. Acta Hydrobiologica Sinica, 2010, 34(2):388-393(in Chinese).

    [15] 李兵, 刘伟, 范赛, 等. 超高效液相色谱-串联质谱法检测螺旋藻保健品中7种微囊藻毒素[J]. 色谱, 2012, 30(6):584-589.

    LI B, LIU W, FAN S, et al. Determination of 7 microcystins in Spirulina health food products by ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2012, 30(6):584-589(in Chinese).

    [16] DAI M, XIE P, LIANG G, et al. Simultaneous determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2008, 862(1):43-50.
    [17] 沈坚, 张爱芝, 曹丽丽, 等. 超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素[J]. 理化检验-化学分册, 2014, 50(5):606-610.

    SHEN J, ZHANG A Z, CAO L L, et al. Simultaneous UHPLC-MS/MS determination of 3 microcystic algae toxins in field snail[J]. Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2014, 50(5):606-610(in Chinese).

    [18] 汪靖, 郑竟, 鄢灵君, 等. 福建沿海市售海产贝类微囊藻毒素的污染状况[J]. 环境与职业医学, 2016, 33(11):1037-1042.

    WANG J, ZHENG J, YAN L J, Pollution status of microcystins in retail marine shellfish in coastal cities of Fujian[J]. Journal of Environmental & Occupational Medicine, 2016, 33(11):1037-1042(in Chinese).

    [19] 虞锐鹏, 陶冠军, 贡小清, 等. 高效液相色谱-质谱联用方法测定背角无齿蚌体内微囊藻毒素[J]. 分析测试学报, 2007, 26(5):671-674.

    YU R P, TAO G J, GONG X Q, et al. Determination of microcystins in Anodonta Woodiana by high performance liquid chromatography combined with electrospray ionization mass spectrometry[J]. Journal of Instrumental Analysis, 2007, 26(5):671-674(in Chinese).

    [20] KOPP R, MARE J, MIROSLAVA P, et al. Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpio L.) from a hypertrophic pond with cyanobacterial water bloom[J]. Aquaculture Research, 2009, 40(15):1683-1693.
    [21] 薛庆举, 苏小妹, 谢丽强. 蓝藻毒素对底栖动物的毒理学研究进展[J]. 生态学报, 2015, 35(14):4570-4578.

    XUE Q J, SU X M, XIE L Q. Advances on cyanotoxin toxicology of zoobenthos[J]. Acta Ecologica Sinica, 2015, 35(14):4570-4578(in Chinese).

    [22] MILLER M A, KUDELA R M, MEKEBRI A, et al. Evidence for a novel marine harmful algal bloom:Cyanotoxin (microcystin) transfer from land to sea otters[J]. Plos One, 2010, 5(9):e815.
    [23] PREECE E P, MOORE B C, HARDY F J. Transfer of microcystin from freshwater lakes to Puget Sound, WA and toxin accumulation in marine mussels (Mytilus trossulus)[J]. Ecotoxicology & Environmental Safety, 2015, 122:98-105.
    [24] IBELINGS B W, CHORUS I. Accumulation of cyanobacterial toxins in freshwater "seafood" and its consequences for public health:A review[J]. Environmental Pollution, 2007, 150(1):177-192.
    [25] CHEN W, SONG L, GAN N, et al. Sorption, degradation and mobility of microcystins in Chinese agriculture soils:Risk assessment for groundwater protection[J]. Environmental Pollution, 2006, 144(3):752-758.
    [26] SONG H, REICHWALDT E S, GHADOUANI A. Contribution of sediments in the removal of microcystin-LR from water[J]. Toxicon, 2014, 83:84-90.
    [27] HU C L, GAN N Q, HE Z K, et al. A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples[J]. International Journal of Environmental Analytical Chemistry, 2008, 88(4):267-277.
    [28] RUIZ M J, CAMEÁN A M, MORENO I M, et al. Determination of microcystins in biological samples by matrix solid-phase dispersion and liquid chromatography-mass spectrometry[J]. Journal of Chromatography A, 2005, 1073(1):257-262.
    [29] CAZENAVE J, AME M V, KRAUSE E, et al. Uptake, tissue distribution and accumulation of microcystin-RR in Corydoras paleatus, Jenynsia multidentata and Odontesthes bonariensis. A field and laboratory study[J]. Aquatic Toxicology, 2005, 75(2):178-190.
    [30] Commission Decision 2002/657/EC of 12 August 2002, implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of result[Z]. Official Journal of the European Communities, 2002, L221:8-36.
    [31] DE FIGUEIREDO D R, AZEITEIRO U M, ESTEVES S M, et al. Microcystin-producing blooms-a serious global public health issue[J]. Ecotoxicology and Environmental Safety, 2004, 59(2):151-163.
    [32] MALBROUCK C, KESTEMONT P. Effects of microcystins on fish[J]. Environmental Toxicology and Chemistry, 2006, 25(1):72-86.
    [33] 贾军梅, 罗维, 吕永龙. 太湖鲫鱼和鲤鱼体内微囊藻毒素的累积及健康风险[J]. 环境化学, 2014, 33(2):186-193.

    JIA J M, LUO W, LV Y L. Accumulation of microcystins in Carassius auratus and Cyprinus carpio from the Taihu Lake and its human health risk[J]. Environmental Chemistry, 2014, 33(2):186-193(in Chinese).

    [34] 贾军梅, 罗维, 吕永龙. 微囊藻毒素在太湖白鲢体内的累积规律及其影响因素[J]. 生态毒理学报, 2014, 9(2):382-390.

    JIA J M, LUO W, LV Y L. Accumulation of Microcystins in Silver Carp from Taihu Lake and its influencing factors[J]. Asian Journal of Ecotoxicology, 2014, 9(2):382-390(in Chinese).

    [35] 高鸽, 周游, 王小林, 等. 夏秋季太湖4个控藻区鳙体内微囊藻毒素累积分析[J]. 大连海洋大学学报, 2017, 32(3):334-341.

    GAO G,ZHOU Y,WANG X L, et al. Accumulation of microcystins in bighead carp Aristichthys nobilis in four alga control areas of Taihu Lake in summer and autumn[J]. Journal of Dalian Ocean University, 2017, 32(3):334-341(in Chinese).

    [36] 陈隽. 肝毒性微囊藻毒素在巢湖和太湖水生动物体内的生物富集及对水产品安全性的潜在威胁[D]. 北京:中国科学院研究生院, 2006. CHEN J. Bioaccumulation of the hepatotoxic microcystins in aquatic animals of Lakes Chaohu and Taihu with potential risk to the safety of aquatic products[D]. Beijing:Chinese Academy of Sciences, 2006(in Chinese).
    [37] 徐海滨, 孙明, 隋海霞, 等. 江西鄱阳湖微囊藻毒素污染及其在鱼体内的动态研究[J]. 卫生研究, 2003(3):192-194. XU H B, SUN M, SUI H X, et al. Microcystin contamination of fish on Poyang Lake in Jiangxi province[J]. Journal of Hygiene Research, 2003

    (3):192-194(in Chinese).

    [38] 张君倩, 谢志才, 王智, 等. 微囊藻毒素在滇池螺蛳各组织中的积累及动态分布[J]. 长江流域资源与环境, 2011, 20(2):179-184.

    ZHANG J Q, XIE Z C, WANG Z, et al. Bioaccumulation of microcystins in various tissues of the freshwater snail margarya melanioides (an endemic specie of Yunnan province) from a large shallow plateau lake, the eutrophic Lake Dianchi, China[J]. Resources and Environment in the Yangtze Basin, 2011, 20(2):179-184(in Chinese).

    [39] DIETRICH D, HOEGER S. Guidance values for microcystins in water and cyanobacterial supplement products (blue-green algal supplements):A reasonable or misguided approach[J]. Toxicology & Applied Pharmacology, 2005, 203(3):273-289.
    [40] ZHANG D, XIE P, LIU Y, et al. Transfer, distribution and bioaccumulation of microcystins in the aquatic food web in Lake Taihu, China, with potential risks to human health[J]. Science of the Total Environment, 2009, 407(7):2191-2199.
    [41] 杨晓红, 蒲朝文, 张仁平, 等. 水体微囊藻毒素污染对人群的非致癌健康风险[J]. 中国环境科学, 2013, 33(1):181-185.

    YANG X H, PU C W, ZHANG R P, et al. Assessment on non-carcinogenic health risk of microcystins in the water environment of Chongqing[J]. China Environmental Science, 2013, 33(1):181-185(in Chinese).

    [42] GUPTA N, PANT S C, VIJAYARAGHAVAN R, et al. Comparative toxicity evaluation of cyanobacterial cyclic peptide toxin microcystin variants (LR, RR, YR) in mice[J]. Toxicology, 2003, 188(2):285-296.
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  • 收稿日期:  2019-12-08

太湖贝类中微囊藻毒素的测定与健康风险评估

    通讯作者: 鲜啟鸣, E-mail: xianqm@nju.edu.cn
  • 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京, 210023
  • 收稿日期:  2019-12-08
基金项目:

太湖水环境综合治理科研课题基金（TH2016301）和"十三五"水专项课题（2017ZX07204004）资助.

摘要: 本文调查了太湖环境水体贝类中5种微囊藻毒素microcystins（MCs：MC-RR、MC-YR、MC-LR、MC-LF、MC-LW）的残留水平及其健康风险.采集2017年太湖湖区（梅梁湖和南太湖）的贝类样品（河蚌、河蚬），取可食用组织，经冻干处理后，采用80%甲醇/水溶液涡旋振荡提取，高速离心后的上清液通过HLB固相萃取柱富集净化，采用高效液相色谱-串联质谱法测定样品中MCs的含量.结果表明，MCs的质量浓度在5.00—100.0 μg·L-1范围内呈良好线性关系，相关系数r2均大于0.99.MCs在空白贝类组织中的加标回收率范围为72.4%—82.6%，相对标准偏差（RSD）均小于8%；在太湖贝类可食用组织中都能检测到MC-RR和MC-LR两种常见的MCs，河蚌中藻毒素浓度大于河蚬中藻毒素的浓度，梅梁湖河蚌中总藻毒素含量为0.877 mg·kg-1 dw（干重），高于南太湖河蚌中藻毒素的浓度0.690 mg·kg-1 dw.通过人体健康风险评价模型分析，太湖湖区的贝类存在不同程度的健康风险，其中采集的河蚬样品的风险指数较低，而河蚌样品健康风险指数均大于0.7，接近风险阈值，人类食用河蚌水产品具有一定的潜在健康风险.

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