碲化镉量子点诱导氧自由基产生的检测

刘娜; 赵淑锐; 林彤; 冯爽; 牟颖; 韩四海; 黄沛力; 孙志伟

生态毒理学报

碲化镉量子点诱导氧自由基产生的检测

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刘娜, 赵淑锐, 林彤, 冯爽, 牟颖, 韩四海, 黄沛力, 孙志伟. 碲化镉量子点诱导氧自由基产生的检测[J]. 生态毒理学报, 2012, 7(1): 99-106.

引用本文:

刘娜, 赵淑锐, 林彤, 冯爽, 牟颖, 韩四海, 黄沛力, 孙志伟. 碲化镉量子点诱导氧自由基产生的检测[J]. 生态毒理学报, 2012, 7(1): 99-106.

Liu Na, Zhao Shurui, Lin Tong, Feng Shuang, Mu Ying, Han Sihai, Huang Peili, Sun Zhiwei. Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots[J]. Asian journal of ecotoxicology, 2012, 7(1): 99-106.

Citation:

Liu Na, Zhao Shurui, Lin Tong, Feng Shuang, Mu Ying, Han Sihai, Huang Peili, Sun Zhiwei. Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots[J]. Asian journal of ecotoxicology, 2012, 7(1): 99-106.

碲化镉量子点诱导氧自由基产生的检测

1. 首都医科大学公共卫生与家庭医学学院, 北京 100069;
2. 首都医科大学医学实验与测试中心现代药学实验与测试室, 北京 100069;
3. 浙江大学智能系统与控制研究所分析仪器研究中心工业控制技术国家重点实验室, 杭州 310058

作者简介: 刘娜(1985-),女,硕士研究生,研究方向为毒理学,E-mail:liuliu3011580@sohu.com

基金项目:
国家自然科学基金资助项目(31070772)

苏州市科技计划项目(ZXG0920)
中图分类号: X171.5;R12

Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots

1. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China;
2. Pharmaceutical Experimental Test Chamber, Medical Experiment and Test Center, Capital Medical University, Beijing 100069, China;
3. State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, China
Fund Project:

摘要: 随着量子点(quantum dots,QDs)在生物标记和医学影像等领域的应用日益广泛,其环境暴露量逐渐增加,深入探讨QDs的毒性机制具有重要意义。QDs通过产生活性氧(ROS)诱发毒性效应是目前普遍接受的毒作用模式。为了探讨量子点的毒性与所诱发的ROS的种类和数量的关系,选用碲化镉量子点(CdTe QDs和CdTe/ZnS QDs),利用电子顺磁共振技术(EPR)分别测定了CdTe QDs和CdTe/ZnS QDs在无细胞体系中诱导ROS产生的种类和强度;利用EPR法、紫外可见分光光度法和荧光分光光度法分别测定了4、20和100 nmol· mL^-1的CdTe QDs和CdTe/ZnS QDs对超氧阴离子(· O₂^-)和羟基自由基(· OH)产生的促进作用。实验结果显示,CdTe QDs可诱导· O₂^-的产生;CdTe QDs和CdTe/ZnS QDs对· OH与· O₂^-的产生有明显的促进作用,且具有剂量-效应关系。研究表明,量子点可诱导和促进ROS产生,不同结构量子点对ROS的诱导和促进作用不同。
- 量子点 /
- 羟基自由基 /
- 超氧阴离子 /
- 电子顺磁共振
Abstract: With the extensive application of quantum dots (QDs) in biomarker and bioimaging, the amount of human exposure to QDs increases, so the exploration of the mechanism of their biological toxicity is of significant concern. The generation of ROS (reactive oxygen species) induced by QDs is accepted to act as a mechanism to produce toxicity. To investigate the relationship between the toxicity of QDs and the production of ROS, cadmium-telluride QDs (CdTe QDs and CdTe/ZnS QDs) were selected, and the type and intensity of ROS were detected using electron paramagnetic resonance (EPR) when adding various concentrations of CdTe QDs or CdTe/ZnS QDs in the cell-free system. The promotive effect of CdTe QDs or CdTe/ZnS QDs on the production of superoxide anion (·O₂^-) and hydroxyl free radicals (·OH) was studied by EPR, ultraviolet and fluorescence spectrophotometer. Results showed that CdTe QDs could induce the production of·O₂^-. The generation of·OH and·O₂^- could be obviously promoted by CdTe QDs and CdTe/ZnS QDs. The intensity of·OH (or·O₂^-) and the dose of QDs showed a clear dose-effect relationship. It was indicated that QDs could induce and promote the production of ROS. The inducing and promoting effect was dependent on the structure of QDs.
- quantum dots /
- hydroxyl free radicals /
- superoxide anion /
- electron paramagnetic resonance

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刘娜, 赵淑锐, 林彤, 冯爽, 牟颖, 韩四海, 黄沛力, 孙志伟. 碲化镉量子点诱导氧自由基产生的检测[J]. 生态毒理学报, 2012, 7(1): 99-106.

引用本文:

刘娜, 赵淑锐, 林彤, 冯爽, 牟颖, 韩四海, 黄沛力, 孙志伟. 碲化镉量子点诱导氧自由基产生的检测[J]. 生态毒理学报, 2012, 7(1): 99-106.

Citation:

碲化镉量子点诱导氧自由基产生的检测

作者简介: 刘娜(1985-),女,硕士研究生,研究方向为毒理学,E-mail:liuliu3011580@sohu.com
1. 首都医科大学公共卫生与家庭医学学院, 北京 100069;

2. 首都医科大学医学实验与测试中心现代药学实验与测试室, 北京 100069;

3. 浙江大学智能系统与控制研究所分析仪器研究中心工业控制技术国家重点实验室, 杭州 310058

收稿日期: 2011-09-07

基金项目:

国家自然科学基金资助项目(31070772)

苏州市科技计划项目(ZXG0920)

关键词:

摘要: 随着量子点(quantum dots,QDs)在生物标记和医学影像等领域的应用日益广泛,其环境暴露量逐渐增加,深入探讨QDs的毒性机制具有重要意义。QDs通过产生活性氧(ROS)诱发毒性效应是目前普遍接受的毒作用模式。为了探讨量子点的毒性与所诱发的ROS的种类和数量的关系,选用碲化镉量子点(CdTe QDs和CdTe/ZnS QDs),利用电子顺磁共振技术(EPR)分别测定了CdTe QDs和CdTe/ZnS QDs在无细胞体系中诱导ROS产生的种类和强度;利用EPR法、紫外可见分光光度法和荧光分光光度法分别测定了4、20和100 nmol· mL^-1的CdTe QDs和CdTe/ZnS QDs对超氧阴离子(· O₂^-)和羟基自由基(· OH)产生的促进作用。实验结果显示,CdTe QDs可诱导· O₂^-的产生;CdTe QDs和CdTe/ZnS QDs对· OH与· O₂^-的产生有明显的促进作用,且具有剂量-效应关系。研究表明,量子点可诱导和促进ROS产生,不同结构量子点对ROS的诱导和促进作用不同。

English Abstract

Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots

1. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China;
2. Pharmaceutical Experimental Test Chamber, Medical Experiment and Test Center, Capital Medical University, Beijing 100069, China;
3. State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, China

Received Date: 2011-09-07

Fund Project:

Keywords:

Abstract: With the extensive application of quantum dots (QDs) in biomarker and bioimaging, the amount of human exposure to QDs increases, so the exploration of the mechanism of their biological toxicity is of significant concern. The generation of ROS (reactive oxygen species) induced by QDs is accepted to act as a mechanism to produce toxicity. To investigate the relationship between the toxicity of QDs and the production of ROS, cadmium-telluride QDs (CdTe QDs and CdTe/ZnS QDs) were selected, and the type and intensity of ROS were detected using electron paramagnetic resonance (EPR) when adding various concentrations of CdTe QDs or CdTe/ZnS QDs in the cell-free system. The promotive effect of CdTe QDs or CdTe/ZnS QDs on the production of superoxide anion (·O₂^-) and hydroxyl free radicals (·OH) was studied by EPR, ultraviolet and fluorescence spectrophotometer. Results showed that CdTe QDs could induce the production of·O₂^-. The generation of·OH and·O₂^- could be obviously promoted by CdTe QDs and CdTe/ZnS QDs. The intensity of·OH (or·O₂^-) and the dose of QDs showed a clear dose-effect relationship. It was indicated that QDs could induce and promote the production of ROS. The inducing and promoting effect was dependent on the structure of QDs.

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碲化镉量子点诱导氧自由基产生的检测

作者简介: 刘娜(1985-),女,硕士研究生,研究方向为毒理学,E-mail:liuliu3011580@sohu.com

Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots

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碲化镉量子点诱导氧自由基产生的检测

English Abstract

Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots

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