摘要:
为提高Anammox菌对各种操作条件的应变能力, 扩大该技术在实际工程上的应用范围, 对Anammox反应器在不同操作条件下的脱氮性能及其菌群的迁移转化规律进行试验性研究。292 d的实验数据表明, Anammox反应器在充足无机碳源环境、室温环境以及高盐环境下均可高效稳定的运行, 且在室温为 (23±2)℃、污泥量为22 g-MLSS/L下最高氮负荷达20.5 kg/m3·d, 根据DNA结果, 此阶段KU2约占反应器内所有菌群的75%, 说明此类菌群对低温高负荷条件具有很强的生存性。此外, 当进水盐度为30 g/L时, Anammox反应器仍可进行高效脱氮处理, 而DNA结果显示, 此阶段反应器内KU2所占比例降至36.5%, 说明进水中的高盐度对KU2的富集具有消极意义。有关Anammox菌对高盐环境长期适应性及菌群变化的研究尚少, 仍需进一步探讨。
关键词:
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厌氧氨氧化
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脱氮
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室温
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高盐
Abstract:
In order to improve the adaption capacity of the Anammox bacteria on a variety of operating conditions, and also to expand the scope of application in practice, the nitrogen removal performance as well as the shift and transformation of Anammox bacteria population under different environmental conditions were studied.The experimental data of 292 days showed that the Anammox reactor could operate efficiently under the conditions of adequate inorganic carbon source, ambient temperature and high-salt concentration.Under the ambient temperature of (23±2)℃ and sludge concentration of 22 g-MLSS/L, the maximum nitrogen loading rate (NLR) was as high as 20.5 kg/m3·d.According to the DNA results, KU2 accounted for 75% of the microbial population in the Anammox reactor at this running stage, indicating the strong survival capacity of this kind of bacteria under the conditions of low temperature and high NLR.Besides, when the influent NaCl concentration was 30 g/L, Anammox reactor could still be efficient for nitrogen removal, but DNA results exhibited that at this stage the proportion of KU2 in the reactor dropped to 36.5%, indicating that the high salinity water had negative effect on KU2 enrichment.Long-term cultivation of these kinds of Anammox bacteria under high salt concentrations was still poorly understood and should be investigated in the future.
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