玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究

卢娜; 周顺桂; 常明; 倪晋仁

环境工程学报

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卢娜, 周顺桂, 常明, 倪晋仁. 玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究[J]. 环境工程学报, 2007, 1(9): 126-130.

引用本文:

卢娜, 周顺桂, 常明, 倪晋仁. 玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究[J]. 环境工程学报, 2007, 1(9): 126-130.

Lu Na, Zhou Shungui, Chang Ming, Ni Jinren. Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material[J]. Chinese Journal of Environmental Engineering, 2007, 1(9): 126-130.

Citation:

Lu Na, Zhou Shungui, Chang Ming, Ni Jinren. Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material[J]. Chinese Journal of Environmental Engineering, 2007, 1(9): 126-130.

玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究

1.
北京大学环境工程系, 教育部水沙科学重点实验室, 北京 100871
基金项目:
广东省自然科学基金资助项目(5006694)

广东省科技攻关资助项目(2005B10401008)
中图分类号: X703

Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material

1.
Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University， Beijing 100871
Fund Project:

摘要: 以玉米淀粉生产过程中的浸泡液为培养基，摇瓶发酵培养苏云金杆菌生物杀虫剂，通过一系列单因子试验，考察了不同培养条件（种子液的种龄、接种量、浸泡液的含固率、初始pH值、摇床转速、发酵温度及发酵时间）对苏云金杆菌在玉米浸泡液中的生长（菌数增长与芽孢形成）以及发酵液的生物毒效的影响。研究表明，在最佳摇瓶培养条件（种子液种龄10 h，接种量2%，浸泡液含固量3%，初始pH值7.0～7.5，摇床转速200 r/min，发酵温度30℃）下发酵48 h，活菌数和活芽孢数分别可达到7.9×108 CFU/mL和5.5×108 CFU/mL，毒力效价为698.0 IU/μL。本试验可为生物农药的工业化生产提供实用参数。
- 玉米浸泡液 /
- 苏云金芽孢杆菌 /
- 培养条件 /
- 生物杀虫剂
Abstract: Using corn steepwater as medium, the fermentation process of Bacillus thuringiensis subsp. kurstaki 130.1 was studied in shaking flask cultures. Effects of various conditions on the growth of Bt including viable cell growth, sporulation and entomotoxicity were investigated； and the optimum culture conditions were determined with shaking flask cultures. Results showed that the suitable culture conditions were as follows: seed age 10 h, inoculum quantity 2%, solid percentage of corn steepwater 3%, initial pH 7.0 ~7.5, shaking speed 200 r/min, temperature 30 ℃ and culture time 48 h. Under these conditions, viable cells and viable spores counts were 7.9×108 CFU/mL and 5.5×108 CFU/mL respectively, and entomotoxicity was 698.0 IU/μL. This study provided basis for practical production.
- corn steepwater /
- Bacillus thuringiensis /
- culture condition /
- biopesticide

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卢娜, 周顺桂, 常明, 倪晋仁. 玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究[J]. 环境工程学报, 2007, 1(9): 126-130.

引用本文:

卢娜, 周顺桂, 常明, 倪晋仁. 玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究[J]. 环境工程学报, 2007, 1(9): 126-130.

Citation:

玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究

1. 北京大学环境工程系, 教育部水沙科学重点实验室, 北京 100871

基金项目:

广东省自然科学基金资助项目(5006694)

广东省科技攻关资助项目(2005B10401008)

关键词:

摘要: 以玉米淀粉生产过程中的浸泡液为培养基，摇瓶发酵培养苏云金杆菌生物杀虫剂，通过一系列单因子试验，考察了不同培养条件（种子液的种龄、接种量、浸泡液的含固率、初始pH值、摇床转速、发酵温度及发酵时间）对苏云金杆菌在玉米浸泡液中的生长（菌数增长与芽孢形成）以及发酵液的生物毒效的影响。研究表明，在最佳摇瓶培养条件（种子液种龄10 h，接种量2%，浸泡液含固量3%，初始pH值7.0～7.5，摇床转速200 r/min，发酵温度30℃）下发酵48 h，活菌数和活芽孢数分别可达到7.9×108 CFU/mL和5.5×108 CFU/mL，毒力效价为698.0 IU/μL。本试验可为生物农药的工业化生产提供实用参数。

English Abstract

Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material

1. Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University， Beijing 100871

Fund Project:

Keywords:

Abstract: Using corn steepwater as medium, the fermentation process of Bacillus thuringiensis subsp. kurstaki 130.1 was studied in shaking flask cultures. Effects of various conditions on the growth of Bt including viable cell growth, sporulation and entomotoxicity were investigated； and the optimum culture conditions were determined with shaking flask cultures. Results showed that the suitable culture conditions were as follows: seed age 10 h, inoculum quantity 2%, solid percentage of corn steepwater 3%, initial pH 7.0 ~7.5, shaking speed 200 r/min, temperature 30 ℃ and culture time 48 h. Under these conditions, viable cells and viable spores counts were 7.9×108 CFU/mL and 5.5×108 CFU/mL respectively, and entomotoxicity was 698.0 IU/μL. This study provided basis for practical production.

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玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究

Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material

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玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究

English Abstract

Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material

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