一株高产纤维素酶真菌的分离及产酶特性研究
Isolation of an effective cellulose-producing fungus and study of its enzyme-production characteristics
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摘要: 一株高产纤维素酶真菌D1，经纤维素刚果红平板识别、滤纸崩解实验初筛以及液体发酵产酶复筛，从腐烂秸秆中被分离出来。经形态观察和18S rDNA 基因片断分析，被鉴定为草酸青霉(Penicillium oxalicum)。通过单因子及正交实验，D1液态发酵产酶条件得到优化。最佳产酶条件为:碳源为麸皮∶稻草=3∶7（w/w）， 含量2.5%（w/v）；氮源为(NH4)2SO4，含量0.15% (w/v)；温度为35℃；自然pH；产酶时间4 d。在此优化条件下，发酵液中CMC酶活和滤纸酶活可达31.12 IU/mL 、45.28 IU/mL。Abstract: An effective cellulose-producing fungus D1 was isolated from rotten straw after identification on congo red dying plates， primary screening by filter paper decomposing and rescreening by liquid fermentation assays. It was identified as Penicillium oxalicum according to its morphology property and corresponding 18S rDNA sequences. The cellulase production conditions via liquid fermentation using D1 were optimized by the single factor and the resulting orthodox experiments. Results showed that the optimal conditions were as follows: 2.5% (w/v) rice bran and wheat straw (3∶7， w/w) as carbon source， 0.15% (w/w) (NH4)2SO4 as the nitrogen source， culture temperature 35℃， natural pH and fermentation time 4 d. Under the conditions， the maximum activities of CMCase and FPA in fermentation broth could reach 31.12 IU/mL and 45.28 IU/mL， respectively.
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Key words:
- cellulase /
- Penicillium oxalicum /
- CMCase activity /
- enzyme production condition
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