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填埋场中亚硝酸还原酶测定条件的优化

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樊超, 邱忠平, 龚正君, 海维燕, 唐建, 华建军. 填埋场中亚硝酸还原酶测定条件的优化[J]. 环境工程学报, 2012, 6(10): 3747-3751.
引用本文: 樊超, 邱忠平, 龚正君, 海维燕, 唐建, 华建军. 填埋场中亚硝酸还原酶测定条件的优化[J]. 环境工程学报, 2012, 6(10): 3747-3751.
shu
Fan Chao, Qiu Zhongpin, Gong Zhengjun, Hai Weiyan, Tang Jian, Hua Jianjun. Optimization for determination condition of nitrite reductase in landfill[J]. Chinese Journal of Environmental Engineering, 2012, 6(10): 3747-3751.
Citation: Fan Chao, Qiu Zhongpin, Gong Zhengjun, Hai Weiyan, Tang Jian, Hua Jianjun. Optimization for determination condition of nitrite reductase in landfill[J]. Chinese Journal of Environmental Engineering, 2012, 6(10): 3747-3751.
shu

填埋场中亚硝酸还原酶测定条件的优化

  • 1.

    西南交通大学生命科学与工程学院, 成都 610031

  • 2.

    西南交通大学地球科学与环境工程学院, 成都 610031

  • 基金项目:

    四川省科技支撑计划(2011SZ0229)

    中央高校基本科研业务费专项资金科技创新项目(SWJTU12CX052,SWJTU12CX020)

  • 中图分类号: X705

Optimization for determination condition of nitrite reductase in landfill

  • Fund Project:

  • 摘要: 填埋垃圾中的含氮化合物经一系列生物脱氮作用,最终使得填埋场中的氮素得以消减,在这一过程中亚硝酸还原酶起着十分重要的作用。以填埋场中的填埋垃圾为研究对象,在土壤亚硝酸还原酶测定方法的基础上,对亚硝酸还原酶活性测定条件进行了优化。结果表明,填埋垃圾中的亚硝酸还原酶活性测定的最优条件为:垃圾样品风干温度25℃,2mL的1%NaNO2溶液和2.5mL的1%葡萄糖溶液,抽气5min,置于25℃的培养箱中培养24h。优化后的测定条件相对标准偏差(RSD)小于1.27%,表明该方法具有较高的灵敏度和精密度。
    Abstract: Nitrogen compounds in the landfill are removed though a series of biological denitrification,in which the nitrite reductase plays a crucial role.Based on the traditional enzymological methods used for the determination of soil nitrite reductase,the conditions for determination of nitrite reductase were optimized with waste samples of landfill.The results showed that the optimum determination conditions for nitrite reductase in landfill were as follows:25℃ air-dried sample,2.0 mL of 1% NaNO2 and 2.5 mL of 1% glucose,5 min of evacuation time,24 h incubation in 25℃ of culture temperature.The relatively standard deviation(RSD)was less than 1.27% when nitrite reductase was measured under optimization conditions,which indicated higher precision and sensitivity.
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出版历程
  • 收稿日期:  2011-10-25
  • 刊出日期:  2012-10-16
樊超, 邱忠平, 龚正君, 海维燕, 唐建, 华建军. 填埋场中亚硝酸还原酶测定条件的优化[J]. 环境工程学报, 2012, 6(10): 3747-3751.
引用本文: 樊超, 邱忠平, 龚正君, 海维燕, 唐建, 华建军. 填埋场中亚硝酸还原酶测定条件的优化[J]. 环境工程学报, 2012, 6(10): 3747-3751.
shu
Fan Chao, Qiu Zhongpin, Gong Zhengjun, Hai Weiyan, Tang Jian, Hua Jianjun. Optimization for determination condition of nitrite reductase in landfill[J]. Chinese Journal of Environmental Engineering, 2012, 6(10): 3747-3751.
Citation: Fan Chao, Qiu Zhongpin, Gong Zhengjun, Hai Weiyan, Tang Jian, Hua Jianjun. Optimization for determination condition of nitrite reductase in landfill[J]. Chinese Journal of Environmental Engineering, 2012, 6(10): 3747-3751.
shu

填埋场中亚硝酸还原酶测定条件的优化

  • 1. 西南交通大学生命科学与工程学院, 成都 610031
  • 2. 西南交通大学地球科学与环境工程学院, 成都 610031
  • 收稿日期:  2011-10-25
基金项目:

四川省科技支撑计划(2011SZ0229)

中央高校基本科研业务费专项资金科技创新项目(SWJTU12CX052,SWJTU12CX020)

摘要: 填埋垃圾中的含氮化合物经一系列生物脱氮作用,最终使得填埋场中的氮素得以消减,在这一过程中亚硝酸还原酶起着十分重要的作用。以填埋场中的填埋垃圾为研究对象,在土壤亚硝酸还原酶测定方法的基础上,对亚硝酸还原酶活性测定条件进行了优化。结果表明,填埋垃圾中的亚硝酸还原酶活性测定的最优条件为:垃圾样品风干温度25℃,2mL的1%NaNO2溶液和2.5mL的1%葡萄糖溶液,抽气5min,置于25℃的培养箱中培养24h。优化后的测定条件相对标准偏差(RSD)小于1.27%,表明该方法具有较高的灵敏度和精密度。

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