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固定化微生物修复石油污染土壤影响因素研究

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张秀霞, 白雪晶, 徐娜娜, 刘永博, 孔甜甜. 固定化微生物修复石油污染土壤影响因素研究[J]. 环境工程学报, 2013, 7(3): 1156-1162.
引用本文: 张秀霞, 白雪晶, 徐娜娜, 刘永博, 孔甜甜. 固定化微生物修复石油污染土壤影响因素研究[J]. 环境工程学报, 2013, 7(3): 1156-1162.
shu
Zhang Xiuxia, Bai Xuejing, Xu Nana, Liu Yongbo, Kong Tiantian. Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1156-1162.
Citation: Zhang Xiuxia, Bai Xuejing, Xu Nana, Liu Yongbo, Kong Tiantian. Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1156-1162.
shu

固定化微生物修复石油污染土壤影响因素研究

  • 1.

    中国石油大学(华东)化学工程学院环境科学与工程系,青岛 266555

  • 基金项目:

    中国石油科技创新基金资助项目(2009D-5006-07-01)

    中央高校基本科研业务费专项基金项目(27R1104052A)

    青岛市科技发展指导计划项目(KJZD-12-65-jch)

  • 中图分类号: X53

Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil

  • 1.

    Department of Environmental Science and Engineering,College of Chemical Engineering, China University of Petroleum (East China),Qingdao 266555, China

  • Fund Project:

  • 摘要: 针对石油污染土壤修复,利用实验室已筛选的高效石油降解单菌SM-3,以天然有机材料为载体,吸附法制备固定化微生物。将游离与固定化微生物应用于室内花盆模拟修复石油污染土壤,对C/N/P、微生物投加量、石油含量、氧化剂和表面活性剂设计5因素4水平正交实验,探讨不同修复时期各影响因素的重要性顺序,最佳条件下各菌株的修复效果。结果表明,不同微生物在不同降解时期,各影响因素的重要性会发生变化;经过21 d的修复,固定化单菌SM-3石油降解率为22.77%,修复过程中,接种量是最重要的影响因素,营养元素N、P投加影响较大,表面活性剂和氧化剂影响次之。
    Abstract: Aiming to remedy the oil-contaminated soil, high-efficiency oil-degrading single strain SM-3 which had been screened in the laboratory was elected as experimental strain. Use natural organic materials as carriers to obtain immobilized microorganism by adsorption method. Free and immobilized microorganisms were applied to simulate and remedy the oil-contaminated soil in the indoor pot. The orthogonal experiment of five factors (C/N/P, inoculated quantity, oil content, oxidant and surfactant) and four levels was designed to investigate the sequence of the importance of each influencing factor in different remedial periods and the remedial effect of each strain under their respective optimum conditions. The experiment showed that for different microorganisms, the importance of each factor changed in different periods. After the remediation of 21 days, the oil degradation rate of immobilized single strain SM-3 was 22.77%. During the biodegradation, inoculated quantity was the most important factor. Nutrients nitrogen and phosphorus had a relative greater effect on degradation rate compared with the influence of oxidant and surfactant.
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出版历程
  • 收稿日期:  2011-10-13
  • 刊出日期:  2013-03-18
张秀霞, 白雪晶, 徐娜娜, 刘永博, 孔甜甜. 固定化微生物修复石油污染土壤影响因素研究[J]. 环境工程学报, 2013, 7(3): 1156-1162.
引用本文: 张秀霞, 白雪晶, 徐娜娜, 刘永博, 孔甜甜. 固定化微生物修复石油污染土壤影响因素研究[J]. 环境工程学报, 2013, 7(3): 1156-1162.
shu
Zhang Xiuxia, Bai Xuejing, Xu Nana, Liu Yongbo, Kong Tiantian. Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1156-1162.
Citation: Zhang Xiuxia, Bai Xuejing, Xu Nana, Liu Yongbo, Kong Tiantian. Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1156-1162.
shu

固定化微生物修复石油污染土壤影响因素研究

  • 1. 中国石油大学(华东)化学工程学院环境科学与工程系,青岛 266555
  • 收稿日期:  2011-10-13
基金项目:

中国石油科技创新基金资助项目(2009D-5006-07-01)

中央高校基本科研业务费专项基金项目(27R1104052A)

青岛市科技发展指导计划项目(KJZD-12-65-jch)

摘要: 针对石油污染土壤修复,利用实验室已筛选的高效石油降解单菌SM-3,以天然有机材料为载体,吸附法制备固定化微生物。将游离与固定化微生物应用于室内花盆模拟修复石油污染土壤,对C/N/P、微生物投加量、石油含量、氧化剂和表面活性剂设计5因素4水平正交实验,探讨不同修复时期各影响因素的重要性顺序,最佳条件下各菌株的修复效果。结果表明,不同微生物在不同降解时期,各影响因素的重要性会发生变化;经过21 d的修复,固定化单菌SM-3石油降解率为22.77%,修复过程中,接种量是最重要的影响因素,营养元素N、P投加影响较大,表面活性剂和氧化剂影响次之。

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