高级搜索

微生物矿化修复重金属污染土壤

,  , 

downloadPDF

上一篇

下一篇

许燕波, 钱春香, 陆兆文. 微生物矿化修复重金属污染土壤[J]. 环境工程学报, 2013, 7(7): 2763-2768.
引用本文: 许燕波, 钱春香, 陆兆文. 微生物矿化修复重金属污染土壤[J]. 环境工程学报, 2013, 7(7): 2763-2768.
shu
Xu Yanbo, Qian Chunxiang, Lu Zhaowen. Remediation of heavy metal contaminated soils by bacteria biomineralization[J]. Chinese Journal of Environmental Engineering, 2013, 7(7): 2763-2768.
Citation: Xu Yanbo, Qian Chunxiang, Lu Zhaowen. Remediation of heavy metal contaminated soils by bacteria biomineralization[J]. Chinese Journal of Environmental Engineering, 2013, 7(7): 2763-2768.
shu

微生物矿化修复重金属污染土壤

  • 1.

    东南大学材料科学与工程学院, 南京 211189

  • 2.

    东南大学江苏省土木工程材料重点实验室, 南京 211189

  • 基金项目:

    江苏省自然科学基金重点项目(BK2010062)

  • 中图分类号: X53

Remediation of heavy metal contaminated soils by bacteria biomineralization

  • 1.

    School of Materials Science and Engineering, Southeast University, Nanjing 211189, China

  • 2.

    Jiangsu Key Laboratory of Construction Materials, Southeast University, Nanjing 211189, China

  • Fund Project:

  • 摘要: 以选矿厂附近土壤为研究对象,分析了土壤中交换态重金属含量, As、Pb、Cd、Zn和Cu的交换态浓度为14.01、4.95、0.64、33.46和12.95 mg/kg。基于生物矿化原理,利用碳酸盐矿化菌生长代谢过程产生的脲酶来分解底物尿素,产生碳酸根离子,固结重金属离子,使得土壤中活泼的重金属离子转变为碳酸盐矿物态,降低其危险。研究了温度、pH和重金属离子对酶活性的影响,发现环境30℃温度有利于促进酶活性;在弱酸性条件下,底物分解量减少15%;重金属离子在低浓度时对脲酶活性影响不大,浓度提高后对酶活性抑制作用没有加剧。将制备好的微生物矿化修复制剂喷洒于1 000 m2的污染土壤中,实验结果发现,土壤中交换态重金属离子含量在0~20 cm范围内明显减少,As、Pb、Cd、Zn和Cu的交换态浓度分别减少至2.37、1.25、0.31、16.67和3.42 mg/kg。
    Abstract: The soil near the concentration plant was analyzed, while the contents of exchangeable As,Pb,Cd,Zn and Cu contents were 14.01,4.95,0.64,33.46 and 12.95 mg/kg, respectively. Based on the theory of biomineralization, the heavy metal ion was mineralized as stable carbonate to reduce the risk by the CO32- decompounded by the enzyme effect of carbonate-mineralization microbe. Effects of temperature, environment pH and heavy metals on the enzyme activity were studied. The results indicated that the higher temperature was helpful to enhance enzyme activity. In weak acidic medium, the decomposition quantity of the substrate decreased by 15%. The heavy metal at low concentration had a weak effect of inhibition on enzyme activity while the inhibition did not get worse at high concentration. The prepared microorganisms were sprayed on the polluted soil. The results showed that the contents of exchangeable heavy metals in soil decreased obviously and the contents of exchangeable As,Pb,Cd,Zn and Cu were 2.37,1.25,0.31,16.67 and 3.42 mg/kg, respectively.
  • 加载中
  • [1] Tessier A., Campbell P. G. C., Bisson M. Sequential extraction procedure for the speciation of particulate trace metals.Analytical Chemistry, 1979, 51(7):844-851
    [2] 刘玉荣,党志,尚爱安. 污染土壤中重金属生物有效性的植物指示法研究. 环境污染与防治,2003, 25(4):215-217 Liu Y.R., Dang Z, Shang A.A. Study on bioavailability of heavy metals in polluted soil using phytoindicating. Environmental Pollution and Control,2003,25(4):215-217 (in Chinese)
    [3] 付善明,周永章,赵宇鴳等. 广东大宝山铁多金属矿废水对河流沿岸土壤的重金属污染. 环境科学,2007, 28(4):805-812 Fu S.M., Zhou Y.Z., Zhao Y.Y., et al. Study on heavy metals in soils contaminated by acid mine drainage from dabaoshan mine, guangdong. Environmental Science,2007, 28(4):805-812 (in Chinese)
    [4] S. Van Roy, Vanbroekhoven K., Dejonghe W., et al. Immobilization of heavy metals in the saturated zone by sorption and in situ bioprecipitation processes. Hydrometallurgy,2006, 83(1):195-203
    [5] Yoshiko Fujita, George D. R., et al. Geochimica et al. Strontium incorporation into calcite generated by bacterial ureolysis. Geochimica et Cosmochimica Acta,2004, 68(15):3261-3270
    [6] Ivan S., Egon M. Homogeneous precipitation by enzyme-catalyzed reactions-strontium and barium carbonates. Chemistry of Materials,2003, 15(6):1322-1326
    [7] Francesca P., Paola Z. Heavy metal coprecipitation with hydrozincite from mine waters caused by photosynthetic microorganisms. Applied and Environmental Microbiology,2000, 66(11):5092-5098
    [8] 王瑞兴,钱春香,吴淼等. 微生物矿化固结土壤中重金属研究. 功能材料,2007, 9 (38):1523-1527 Wang R.X., Qian C.X., Wu M, Cheng L. Study on heavy metals in soil mineralized by bacteria. Functional Materials,2007, 9(38):1523-1527 (in Chinese)
    [9] 成亮,钱春香,王瑞兴,等. 碳酸盐矿化菌株固结土壤Cd2+的生物矿化过程. 硅酸盐学报,2008, 36(S1): 216-222 Cheng L., Qian C.X., Wang R.X., et al. Bioremedidation process of Cd2+ removal from soil by bacteria biomineralization.Chinese Ceramic Society,2008, 36(S1),:216-222 (in Chinese)
    [10] Wang L.N., Huo J.C., Liu S.X., et al. A new route to the synthesis of barium carbonate crystals by the induction of Bacillus pasteurii. Chinese Journal of Structural Chemistry,2011, 30(5):738-742
    [11] 林大义. 土壤学实验指导. 北京:中国林业出版社,2004
    [12] 李亚星,徐秋明,曹一平,等. 分光光度法测定树脂包衣尿素溶出的研究. 中国土壤与肥料,2010, (1):84-87 Li Y.X., Xu Q.M., Cao Y.P., Yang Y.B. Colorimetric estimation of urea release rate in coated urea. Soil and Fertilizer Science in China,2010, (1):84-87 (in Chinese)
    [13] 韩桂琪,王彬,徐卫红,等. 重金属Cd、Zn、Cu、Pb复合污染对土壤微生物和酶活性的影响. 水土保持学报,2010, 24(5):238-242 Han G.Q., Wang B, Xu W.H., et al. Effect of heavy metal combined pollution on soil microbial indicators and soil enzymatic activity. Journal of Soil and Water Conservation,2010, 24(5):238-242(in Chinese)
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2281
  • HTML全文浏览数:  1453
  • PDF下载数:  1058
  • 施引文献:  0
出版历程
  • 收稿日期:  2012-05-16
  • 刊出日期:  2013-07-18
许燕波, 钱春香, 陆兆文. 微生物矿化修复重金属污染土壤[J]. 环境工程学报, 2013, 7(7): 2763-2768.
引用本文: 许燕波, 钱春香, 陆兆文. 微生物矿化修复重金属污染土壤[J]. 环境工程学报, 2013, 7(7): 2763-2768.
shu
Xu Yanbo, Qian Chunxiang, Lu Zhaowen. Remediation of heavy metal contaminated soils by bacteria biomineralization[J]. Chinese Journal of Environmental Engineering, 2013, 7(7): 2763-2768.
Citation: Xu Yanbo, Qian Chunxiang, Lu Zhaowen. Remediation of heavy metal contaminated soils by bacteria biomineralization[J]. Chinese Journal of Environmental Engineering, 2013, 7(7): 2763-2768.
shu

微生物矿化修复重金属污染土壤

  • 1.  东南大学材料科学与工程学院, 南京 211189
  • 2.  东南大学江苏省土木工程材料重点实验室, 南京 211189
  • 收稿日期:  2012-05-16
基金项目:

江苏省自然科学基金重点项目(BK2010062)

摘要: 以选矿厂附近土壤为研究对象,分析了土壤中交换态重金属含量, As、Pb、Cd、Zn和Cu的交换态浓度为14.01、4.95、0.64、33.46和12.95 mg/kg。基于生物矿化原理,利用碳酸盐矿化菌生长代谢过程产生的脲酶来分解底物尿素,产生碳酸根离子,固结重金属离子,使得土壤中活泼的重金属离子转变为碳酸盐矿物态,降低其危险。研究了温度、pH和重金属离子对酶活性的影响,发现环境30℃温度有利于促进酶活性;在弱酸性条件下,底物分解量减少15%;重金属离子在低浓度时对脲酶活性影响不大,浓度提高后对酶活性抑制作用没有加剧。将制备好的微生物矿化修复制剂喷洒于1 000 m2的污染土壤中,实验结果发现,土壤中交换态重金属离子含量在0~20 cm范围内明显减少,As、Pb、Cd、Zn和Cu的交换态浓度分别减少至2.37、1.25、0.31、16.67和3.42 mg/kg。

English Abstract

    全文HTML

参考文献 (13)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心