高级搜索

内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛

,  ,  , 

downloadPDF

上一篇

下一篇

蒋晶君, 吴斌, 曹张军, 周美华. 内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛[J]. 环境工程学报, 2014, 8(10): 4415-4418.
引用本文: 蒋晶君, 吴斌, 曹张军, 周美华. 内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛[J]. 环境工程学报, 2014, 8(10): 4415-4418.
shu
Jiang Jingjun, Wu Bin, Cao Zhangjun, Zhou Meihua. Action analysis of hydrogen peroxide (H2O2) produced by Stenotrophomonas maltophilia DHHJ to feather degradation[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4415-4418.
Citation: Jiang Jingjun, Wu Bin, Cao Zhangjun, Zhou Meihua. Action analysis of hydrogen peroxide (H2O2) produced by Stenotrophomonas maltophilia DHHJ to feather degradation[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4415-4418.
shu

内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛

  • 1.

    东华大学生态纺织教育部重点实验室, 上海 201620

  • 2.

    东华大学化学化工与生物工程学院, 上海 201620

  • 3.

    东华大学环境科学与工程学院, 上海 201620

  • 基金项目:

    国家自然科学基金资助项目（31000989）

    中央高校基本科研业务费专项资金（2232014D3-23）

    高等学校博士学科点专项科研基金资助项目（20090075110007）

  • 中图分类号: X71

Action analysis of hydrogen peroxide (H2O2) produced by Stenotrophomonas maltophilia DHHJ to feather degradation

  • 1.

    Key Laboratory of Science & Technology of Eco-Textile (Donghua University), Ministry of Education, Shanghai 201620, China

  • 2.

    College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China

  • 3.

    College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, China

  • Fund Project:

  • 摘要: 过氧化氢为好氧微生物代谢的中间产物。本研究考察了 Stenotrophomonas maltophilia DHHJ代谢H2O2规律及体内外过氧化氢对酶降解羽毛的促进作用，该菌与代谢相关酶SOD及CAT随发酵时间延长而增长。H2O2含量随培养时间延长而升高，84 h后可达71 μg/mL。该菌也可在低浓度下提高酶对羽毛的降解效率，在含量0.015%，即150 μg/mL时，可将降解率从17%提高到25%以上。结果为微生物降解羽毛机理研究提供一条新的线索，同时也可为羽毛生物炼制产业化应用提供新思路。
    Abstract: Feather is a kind of important bio-waste,because the degraded feather product can be applied in many fields.The mechanism underlying bio-degradation of feather is still not clear.In this study,hydrogen peroxide was found to be produced in feather broth inoculated with Stenotrophomonas maltophilia DHHJ.In vitro assay showed that feathers degradation ratio increased from 17% to 25% by a low concentration of hydrogen peroxide (0.015%,about 150 μg/mL).In vivo condition,the H2O2 concentration gradually increased to 71 μg/mL at 84 h during the culture.These results suggest that hydrogen peroxide produced by bacteria is an important factor for feather degradation.
  • 加载中
  • [1] Gupta R.,Ramnani P.Microbial keratinases and their prospective applications:An overview.Applied Microbiology and Biotechnology,2006,70(1):21-33
    [2] Brandelli A.,Daroit D.,Riffel A.Biochemical features of microbial keratinases and their production and applications.Applied Microbiology and Biotechnology,2010,85(6):1735-1750
    [3] 李江,石鹏君,张王照,等.弗氏链霉菌丝氨酸蛋白酶基因的克隆及表达.生物工程学报,2005,21(5):782-788 Li Jiang,Shi Pengjun,Zhang Wangzhao,et al.Gene cloning and expression of serine protease SFP2 from streptomyces fradiae var.k11.Chinese Journal of Biotechnology,2005,21(5):782-788(in Chinese)
    [4] 梁斌,邢述,付学奇,等.角蛋白酶基因 ker B 的提取、克隆及表达.吉林大学学报(理学版),2003,41(3):365-368 Liang Bin,Xing Shu,Fu Xueqi,et al.Extraction,cloning and expression of keratinase gene ker B.Journal of Jilin University (Science Edition),2003,41(3):365-368(in Chinese)
    [5] Lin H.H.,Yin L.J.,Jiang S.T.Cloning,expression,and purification of Pseudomonas aeruginosa keratinase in Escherichia coli AD494 (DE3) pLysS expression system.Journal of Agricultural and Food Chemistry,2009,57(9):3506-3511
    [6] Radha S.,Gunasekaran P.Purification and characterization of keratinase from recombinant Pichia and Bacillus strains.Protein Expression and Purification,2009,64(1):24-31
    [7] Hu H.,Gao J.,He J.et al.Codon optimization significantly improves the expression level of a keratinase gene in pichia pastoris.PloS One,2013,8(3):e58393
    [8] 王晶,陈丽,曹张军,等.一株羽毛角蛋白降解菌的分离与鉴定.农业环境科学学报,2007,26(S1):105-109 Wang Jing,Chen Li,Cao Zhangjun,et al.Isolation and characterization of a strain of new feather-degrading bacteria.Journal of Agro-Environment Science,2007,26(S1):105-109(in Chinese)
    [9] Cao Z.J.,Zhang Q.,Wei D.K.,et al.Characterization of a novel Stenotrophomonas isolate with high keratinase activity and purification of the enzyme.Journal of Industrial Microbiology and Biotechnology,2009,36(2):181-188
    [10] 张淑芳,孔红梅,秦梅.过氧化氢-高碘酸钾-孔雀绿体系分光光度法测定痕量过氧化氢.曲阜师范大学学报 (自然科学版),1998,24(4):122-123 Zhang Shufang,Kong Hongmei,Qin Mei.Hydrogen peroxide-potassium periodate-Malachite Green System spectrophotometric determination of trace hydrogen peroxide.Journal of Qufu Normal University (Science Edition),1998,24(4):122-123(in Chinese)
    [11] 鲁丹,曹张军,周美华.嗜麦芽寡养单胞菌降解羽毛5L发酵罐工业小试过程研究.环境工程学报,2009,3(8):1513-1516 Lu Dan,Cao Zhangjun,Zhou Meihua.Study on Stenotrophomonas maltophilia DHHJ fermentation in 5L fermenter pilot plant.Chinese Journal of Environmental Engineering,2009,3(8):1513-1516(in Chinese)
    [12] Malviya H.K.,Rajak R.C.,Hasija S.K.Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatin factory,Jabalpur,India.Mycopathologia,1992,120(1):1-4
    [13] Bockle B.,Muller R.Reduction of disulfide bonds by streptomyces pactum during growth on chicken feathers.Applied & Environmental Microbiology,1997,63(2):790-792
    [14] Kunert J.Biochemical mechanism of keratin degradation by the actinomycete Streptomyces fradiae and the fungus Microsporum gypseum:A comparison.Journal of Basic Microbiology,1989,29(9):597-604
    [15] 张启,王晶,曹张军,等.嗜麦芽窄食单胞菌( Stenotrophomonas maltophilia )DHHJ分解角蛋白的生化机制初探.河北农业大学学报,2008,31(2):70-73,82 Zhang Qi,Wang Jing,Cao Zhangjun,et al.A primary study on the biochemical mechanism of degrading keratins by Stenotrophomonas maltophilia DHHJ.Journal of Agricultural University of Hebei,2008,31(2):70-73,82(in Chinese)
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1888
  • HTML全文浏览数:  1178
  • PDF下载数:  1292
  • 施引文献:  0
出版历程
  • 收稿日期:  2013-12-23
  • 刊出日期:  2014-09-28
蒋晶君, 吴斌, 曹张军, 周美华. 内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛[J]. 环境工程学报, 2014, 8(10): 4415-4418.
引用本文: 蒋晶君, 吴斌, 曹张军, 周美华. 内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛[J]. 环境工程学报, 2014, 8(10): 4415-4418.
shu
Jiang Jingjun, Wu Bin, Cao Zhangjun, Zhou Meihua. Action analysis of hydrogen peroxide (H2O2) produced by Stenotrophomonas maltophilia DHHJ to feather degradation[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4415-4418.
Citation: Jiang Jingjun, Wu Bin, Cao Zhangjun, Zhou Meihua. Action analysis of hydrogen peroxide (H2O2) produced by Stenotrophomonas maltophilia DHHJ to feather degradation[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4415-4418.
shu

内源过氧化氢(H2O2)对Stenotrophomonas maltophilia DHHJ降解羽毛

  • 1.  东华大学生态纺织教育部重点实验室, 上海 201620
  • 2.  东华大学化学化工与生物工程学院, 上海 201620
  • 3.  东华大学环境科学与工程学院, 上海 201620
  • 收稿日期:  2013-12-23
基金项目:

国家自然科学基金资助项目（31000989）

中央高校基本科研业务费专项资金（2232014D3-23）

高等学校博士学科点专项科研基金资助项目（20090075110007）

摘要: 过氧化氢为好氧微生物代谢的中间产物。本研究考察了 Stenotrophomonas maltophilia DHHJ代谢H2O2规律及体内外过氧化氢对酶降解羽毛的促进作用，该菌与代谢相关酶SOD及CAT随发酵时间延长而增长。H2O2含量随培养时间延长而升高，84 h后可达71 μg/mL。该菌也可在低浓度下提高酶对羽毛的降解效率，在含量0.015%，即150 μg/mL时，可将降解率从17%提高到25%以上。结果为微生物降解羽毛机理研究提供一条新的线索，同时也可为羽毛生物炼制产业化应用提供新思路。

English Abstract

    全文HTML

参考文献 (15)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心