应用混料实验设计制备生猪养殖发酵床复合菌剂

钟仁方, 吴祖芳. 应用混料实验设计制备生猪养殖发酵床复合菌剂[J]. 环境工程学报, 2014, 8(10): 4427-4432.
引用本文: 钟仁方, 吴祖芳. 应用混料实验设计制备生猪养殖发酵床复合菌剂[J]. 环境工程学报, 2014, 8(10): 4427-4432.
Zhong Renfang, Wu Zufang. Preparation of microbial fermenter of fermentation bed in pig culture by mixture design[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4427-4432.
Citation: Zhong Renfang, Wu Zufang. Preparation of microbial fermenter of fermentation bed in pig culture by mixture design[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4427-4432.

应用混料实验设计制备生猪养殖发酵床复合菌剂

  • 基金项目:

    宁波市农业攻关项目(2010C10058)

  • 中图分类号: X172

Preparation of microbial fermenter of fermentation bed in pig culture by mixture design

  • Fund Project:
  • 摘要: 畜禽粪便中包含很多有害成份在自然条件下分解速率慢、效率低。以发酵产物的蛋白酶活、对吲哚和粪臭素降解能力为评价指标,采用混料试验设计优化4株优良菌种的复配条件并建立数学回归方程,通过软件拟合优化和实验验证,最后得到发酵床使用的菌剂最优配比为放线菌G10%、光合细菌H40%、光合细菌L23%和枯草芽孢杆菌Y27%;根据此配比得到的发酵菌剂其蛋白酶酶活、吲哚和粪臭素降解率分别为11.3 u/g、61.8%和41.3%。
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  • [1] 李远.我国规模化畜禽养殖业存在的环境问题与防治对策.上海环境科学,2002,21(10):597-599 Li Yuan.Existing problems and prevention and control coun- termeasures of intensivelivestockcultivation in China.Shanghai Environmental Sciences,2002,21(10):597-599(in Chinese)
    [2] 环境保护部.2009年中国环境状况公报,2010
    [3] Zhang C.,Zeng G.M.,Yuan L.Aerobic degradation of bisphenol A by Achromobacter xylosoxidans strain B-16 isolated from compost leachate of municipal solid waste.Chemosphere,2007,68(1):181-190
    [4] Petric I.,Helic' A.,Avdic E.A.Evolution of process parameters and determination of kinetics for co-composting of organic fraction of municipal solid waste with poultry manure.Bioresource Technology,2012,117:107-116
    [5] Korniowicz-Kowalska T.,Bohacz J.Biodegradation of keratin waste:Theory and practical aspects.Waste Management,2011,31(8):1689-1701
    [6] 李凤,贺稚非,李洪军.公猪膻味物质粪臭素Ⅰ相代谢产物研究进展.食品工业科技,2012,33(1):404-408 Li Feng,He Zhifei,Li Hongjun.Review of phaseⅠmetabolites of skatole in boar taint.Science and Technology of Food Industry,2012,33(1):404-408(in Chinese)
    [7] 张庆宁,胡明,朱荣生,等.生态养猪模式中发酵床优势细菌的微生物学性质及其应用研究.山东农业科学,2009,(4):99-105 Zhang Qingning,Hu Ming,Zhu Rongsheng,et al.Microbial properties and application of superior bacteria in deep-litter ecosystem for piggery.Shandong Agricultural Sciences,2009,(4):99-105(in Chinese)
    [8] 胡秀双.微生态发酵床养猪技术的应用.养殖技术顾问,2008,(4):14-17 Hu Xiushuang.Application of microbial fermentation bed pig.Technical Advisor for Animal Husbandry,2008,(4):14-17(in Chinese)
    [9] 黄仁术.畜禽粪污除臭剂的研究与应用.广东农业科学,2010,37(7):177-179 Huang Renshu.Research and application of Livestoc dung deodorant.Guangdong Agricultural Sciences,2010,37(7):177-179(in Chinese)
    [10] 朱洪,常志州,王世梅,等.基于畜禽废弃物管理的发酵床技术研究Ⅱ.接种剂的应用效果研究.江苏农业科学,2007,(2):228-232 Zhu Hong,Chang Zhizhou,Wang Shimei,et al.Study on deep litter system for management of livestock manure evaluation on effects of inoculant.Jiangsu Agricultural Sciences,2007,(2):228-232(in Chinese)
    [11] 简保权,朱舒平,邓昌彦,等.猪粪堆肥过程中NH3和H2S的释放及除臭微生物的筛选研究.农业工程学报,2006,22(2):183-196 Jian Baoquan,Zhu Shuping,Deng Changyan,et al.Studies on the characteristics of ammonia and hydrogen sulfide volatilization during composting of pig manure and screening of deodorizing microorganisms.Transactions of the Chinese Society of Agricultural Engineering,2006,22(2):183-196(in Chinese)
    [12] 席北斗,刘鸿亮,孟伟,等.垃圾堆肥高效复合微生物菌剂的制备.环境科学研究,2003,16(2):58-60 Xi Beudou,Liu Hongliang,Meng Wei,et al.Study on preparation technology of complex microbial community in composting process.Research of Environmental Sciences,2003,16(2):58-60(in Chinese)
    [13] 刘睿,倪光远,万楚筠,等.应用混料实验设计制备秸秆复合降解菌剂.中国生物工程杂志,2009,29(9):50-55 Liu Rui,Ni Guangyuan,Wan Chuyun,et al.Application of mixture design for compound microbial inoculants in straw degradation.China Biotechnology,2009,29(9):50-55(in Chinese)
    [14] 毛君,尹航,崔闯,等.基于Minitab确定最佳参数的实验设计.煤矿机械,2008,29(8):14-16 Mao Jun,Yin Hang,Cui Chuang,et al.Based on Minitab confirmed most appropriate parameter of design of experiment.Mine Machinery,2008,29(8):14-16(in Chinese)
    [15] 周剑忠,黄开红,董明盛,等.混料设计在藏灵菇奶纯培养发酵剂配方设计中的应用.中国农业科学,2008,41(3):816-822 Zhou Jianzhong,Huang Kaihong,Dong Mingsheng,et al.Application of mixture design to design of formulation of pure cultures in Tibetan kefir.Scientia Agricultura Sinica,2008,41(3):816-822(in Chinese)
    [16] 吴祖芳,张泽瑞,翁佩芳.降解畜禽废物光合细菌的筛选及其代谢特性.生物技术,2011,21(6):70-74 Wu Zufang,Zhang Zerui,Weng Peifang.A screening and metabolic characteristics of photosynthetic bacteria for animal manure degradation.Biotechnology,2011,21(6):70-74(in Chinese)
    [17] 毕泗伟.生猪养殖废物发酵菌筛选及发酵性能评价.宁波:宁波大学硕士学位论文,2013 Bi Siwei.Screening of fermentation bacteria of pig farming waste and performance evaluation of fermentation.Ningbo:Master Dissertation of Ningbo Universty,2013(in Chinese)
    [18] 于文涛,顾磊,唐美华,等.分光光度法测定焦化废水中的吲哚.分析化学,2005,33(5):680-682 Yu Wentao,Gu Lei,Tang Meihua,et al.Novel method for spectrophotometric determination of indole in coal coking wastewater.Chinese Journal of Analytical Chemistry,2005,33(5):680-682(in Chinese)
    [19] 李凤.公猪膻味物质粪臭素的乳酸菌降解研究.重庆:西南大学博士学位论文,2011 Li Feng.Deradation of skatole that caused boar taint by lactic acid bacteria.Chongqing:Doctor Dissertation of Southwest University,2011(in Chinese)
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出版历程
  • 收稿日期:  2013-10-29
  • 刊出日期:  2014-09-28
钟仁方, 吴祖芳. 应用混料实验设计制备生猪养殖发酵床复合菌剂[J]. 环境工程学报, 2014, 8(10): 4427-4432.
引用本文: 钟仁方, 吴祖芳. 应用混料实验设计制备生猪养殖发酵床复合菌剂[J]. 环境工程学报, 2014, 8(10): 4427-4432.
Zhong Renfang, Wu Zufang. Preparation of microbial fermenter of fermentation bed in pig culture by mixture design[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4427-4432.
Citation: Zhong Renfang, Wu Zufang. Preparation of microbial fermenter of fermentation bed in pig culture by mixture design[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4427-4432.

应用混料实验设计制备生猪养殖发酵床复合菌剂

  • 1. 宁波大学海洋学院, 宁波 315211
基金项目:

宁波市农业攻关项目(2010C10058)

摘要: 畜禽粪便中包含很多有害成份在自然条件下分解速率慢、效率低。以发酵产物的蛋白酶活、对吲哚和粪臭素降解能力为评价指标,采用混料试验设计优化4株优良菌种的复配条件并建立数学回归方程,通过软件拟合优化和实验验证,最后得到发酵床使用的菌剂最优配比为放线菌G10%、光合细菌H40%、光合细菌L23%和枯草芽孢杆菌Y27%;根据此配比得到的发酵菌剂其蛋白酶酶活、吲哚和粪臭素降解率分别为11.3 u/g、61.8%和41.3%。

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