高级搜索

利用MFC阳极室暗发酵培养沼泽红假单胞菌

,  ,  ,  , 

downloadPDF

上一篇

下一篇

王兴祖, 程翔, 孙德智, 任以伟, 徐贵华. 利用MFC阳极室暗发酵培养沼泽红假单胞菌[J]. 环境工程学报, 2014, 8(10): 4492-4496.
引用本文: 王兴祖, 程翔, 孙德智, 任以伟, 徐贵华. 利用MFC阳极室暗发酵培养沼泽红假单胞菌[J]. 环境工程学报, 2014, 8(10): 4492-4496.
shu
Wang Xingzu, Cheng Xiang, Sun Dezhi, Ren Yiwei, Xu Guihua. Dark culture of Rhodopseudomonas palustris using MFC reactor[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4492-4496.
Citation: Wang Xingzu, Cheng Xiang, Sun Dezhi, Ren Yiwei, Xu Guihua. Dark culture of Rhodopseudomonas palustris using MFC reactor[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4492-4496.
shu

利用MFC阳极室暗发酵培养沼泽红假单胞菌

  • 1.

    北京林业大学污染水体源控与生态修复工程研究中心, 北京 100083

  • 2.

    中国科学院重庆绿色智能技术研究院, 重庆 401122

  • 基金项目:

    国家自然科学基金资助项目（51008025，51008023，5110-8439）

    重庆市科技攻关计划项目（CSTC2012GGC20001）

  • 中图分类号: X172

Dark culture of Rhodopseudomonas palustris using MFC reactor

  • 1.

    Research Center for Water Pollution Source Control and Bioremediation, Beijing Forestry University, Beijing 100083, China

  • 2.

    Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 401122, China

  • Fund Project:

  • 摘要: 为了克服光合细菌光培养时的光衰减及高能耗等问题，考查了将微生物燃料电池（MFC）作为培养装置黑暗培养光合细菌的可行性。结果表明，MFC有利于菌株W1的优势生长，接种3 d后MFC内光合细菌浓度即可达到715 mg/L，而空白系统中不到308 mg/L。荧光原位杂交分析表明，MFC体系中的杂菌含量小于4%，远低于空白的33%。利用MFC进行光合细菌培养时，最大输出电压和最大输出功率可分别达到487 mV和56 mW/m2。MFC促进光合细菌生长的原因可能在于对体系兼氧环境的维持，在MFC体系中氧化还原电位始终处于200~-300 mV之间，有利于兼氧光合细菌的优势生长。
    Abstract: To solve the problems of light attenuation and high-energy cost during cultivation of photosynthetic bacteria,we investigated the feasibility whether microbial fuel cell (MFC) can be used as the cultivation device for dark culture of Rhodopseudomonas palustris W1.The investigation results showed that the biomass of strain W1 reached 715 mg/L in the MFC and was below 308 mg/L in control reactor after 3-d growth.Thus,the MFC is conducive to the dominant growth of strain W1.Fluorescent in situ hybridization analysis revealed that the composition percentage of contaminating bacteria was lower than 4% in MFC and higher than 33% in control reactor.During the cultivation of photosynthetic bacteria,the obtained maximum voltage and power density of MFC were 487 mV and 56 mW/m2,respectively.Moreover,the function that MFC promotes the dominant growth of strain W1 can be interpreted as follows:MFC is able to maintain anoxic environment for the dominant growth of photosynthetic bacteria (an oxidation reduction potential in a range from 200 to -300 mV).
  • 加载中
  • [1] 张光明,赵微.光合细菌内高价值物质研究进展.东北农业大学学报,2013,44(7):156-160 Zhang Guangming,Zhao Wei.Research development of high-value substances in photosynthetic bacteria.Journal of Northeast Agricultural University,2013,44(7):156-160(in Chinese)
    [2] 任丽滨,杨晓瑞,陈晓晔,等.光合细菌利用沼液废水制氢的影响因素.环境工程学报,2013,7(3):869-872 Ren Libin,Yang Xiaorui,Chen Xiaoye,et al.Influencing factors of hydrogen producing by photosynthetic bacteria using biogas slurry.Chinese Journal of Environmental Engineering,2013,7(3):869-872(in Chinese)
    [3] Hubas C.,Jesus B.,Passarelli C.,et al.Tools providing new insight into coastal anoxygenic purple bacterial mats:review and perspectives.Research in Microbiology,2011,162(9):858-868
    [4] Ponsano E.H.G.,Paulino C.Z.,Pinto M.F.Phototrophic growth of Rubrivivax gelatinosus in poultry slaughterhouse wastewater.Bioresource Technology,2008,99(9):3836-3842
    [5] Tada C.,Miah M.S.,Tsukahara K.,et al.Simultaneous methanogenesis and phototrophic bacterial growth in relatively dry sewage sludge under light.The Journal of General and Applied Microbiology,2005,51(1):27-33
    [6] Harada N.,Nishiyama M.,Matsumoto S.Inhibition of methanogens increases photo-dependent nitrogenase activities in anoxic paddy soil amended with rice straw.FEMS Microbiology Ecology,2001,35(3):231-238
    [7] Akkerman I.,Janssen M.,Rocha J.,et al.Photobiological hydrogen production:photochemical efficiency and bioreactor design.International Journal of Hydrogen Energy,2002,27(11):1195-1208
    [8] Grammel H.,Gilles E.D.,Ghosh R.Microaerophilic cooperation of reductive and oxidative pathways allows maximal photosynthetic membrane biosynthesis in Rhodospirillum rubrum.Applied and Environmental Microbiology,2003,69(11):6577-6586
    [9] Wang X.Z.,Cheng X.,Sun D.Z.,et al.Biodecolorization and partial mineralization of Reactive Black 5 by a strain of Rhodopseudomonas palustris.Journal of Environmental Sciences,2008,20(10):1218-1225
    [10] Honda R.,Fukushi K.,Yamamoto K.Optimization of wastewater feeding for single-cell protein production in an anaerobic wastewater treatment process utilizing purple non-sulfur bacteria in mixed culture condition.Journal of Biotechnology,2006,125(4):565-573
    [11] Xing D.F.,Zuo Y.,Cheng S.A.,et al.Electricity generation by Rhodopseudomonas palustris DX-1.Environmental Science & Technology,2008,42(11):4146-4151
    [12] Rabaey K.,Verstraete W.Microbial fuel cells:novel biotechnology for energy generation.Trends in Biotechnology,2005,23(6):291-298
    [13] Commault A.S.,Lear G.,Packer M.A.,et al.Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells.Bioresource Technology,2013,139:226-234
    [14] 徐丽丽,庄传东,贾友敦,等.光合细菌最佳生长条件筛选.生物技术,2007,17(2):77-82 Xu Lili,Zhuang Chuandong,Jia Youdun,et al.Choice of optimal growth conditions on photosynthetic bacteria.Biotechnology,2007,17(2):77-82(in Chinese)
    [15] 卢海凤,张光明,何春华.一株光合细菌的鉴定及其处理大豆加工废水试验.哈尔滨工业大学学报,2011,43(12):72-76 Lu Haifeng,Zhang Guangming,He Chunhua.The identification of a strain of photosynthetic bacteria and its effect in treating soybean wastewater.Journal of Harbin Institute of Technology,2011,43(12):72-76(in Chinese)
    [16] Matsunaga T.,Hatano T.,Yamada A.,et al.Microaerobic hydrogen production by photosynthetic bacteria in a double-phase photobioreactor.International Journal of Low-carbon Technologies,2012,7(2):97-103
    [17] Chang Y.J.,Chang Y.T.,Hung C.H.The use of magnesium peroxide for the inhibition of sulfate-reducing bacteria under anoxic conditions.Journal of Industrial Microbiology & Biotechnology,2008,35(11):1481-1491
    [18] Yuan Yanli,Conrad R.,Lu Yahai.Responses of methanogenic archaeal community to oxygen exposure in rice field soil.Environmental Microbiology Reports,2009,1(5):347-354
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1724
  • HTML全文浏览数:  1071
  • PDF下载数:  908
  • 施引文献:  0
出版历程
  • 收稿日期:  2013-10-13
  • 刊出日期:  2014-09-28
王兴祖, 程翔, 孙德智, 任以伟, 徐贵华. 利用MFC阳极室暗发酵培养沼泽红假单胞菌[J]. 环境工程学报, 2014, 8(10): 4492-4496.
引用本文: 王兴祖, 程翔, 孙德智, 任以伟, 徐贵华. 利用MFC阳极室暗发酵培养沼泽红假单胞菌[J]. 环境工程学报, 2014, 8(10): 4492-4496.
shu
Wang Xingzu, Cheng Xiang, Sun Dezhi, Ren Yiwei, Xu Guihua. Dark culture of Rhodopseudomonas palustris using MFC reactor[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4492-4496.
Citation: Wang Xingzu, Cheng Xiang, Sun Dezhi, Ren Yiwei, Xu Guihua. Dark culture of Rhodopseudomonas palustris using MFC reactor[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4492-4496.
shu

利用MFC阳极室暗发酵培养沼泽红假单胞菌

  • 1.  北京林业大学污染水体源控与生态修复工程研究中心, 北京 100083
  • 2.  中国科学院重庆绿色智能技术研究院, 重庆 401122
  • 收稿日期:  2013-10-13
基金项目:

国家自然科学基金资助项目（51008025，51008023，5110-8439）

重庆市科技攻关计划项目（CSTC2012GGC20001）

摘要: 为了克服光合细菌光培养时的光衰减及高能耗等问题，考查了将微生物燃料电池（MFC）作为培养装置黑暗培养光合细菌的可行性。结果表明，MFC有利于菌株W1的优势生长，接种3 d后MFC内光合细菌浓度即可达到715 mg/L，而空白系统中不到308 mg/L。荧光原位杂交分析表明，MFC体系中的杂菌含量小于4%，远低于空白的33%。利用MFC进行光合细菌培养时，最大输出电压和最大输出功率可分别达到487 mV和56 mW/m2。MFC促进光合细菌生长的原因可能在于对体系兼氧环境的维持，在MFC体系中氧化还原电位始终处于200~-300 mV之间，有利于兼氧光合细菌的优势生长。

English Abstract

    全文HTML

参考文献 (18)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心