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一株光合细菌的分离鉴定及其产电特性

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吴义诚, 肖勇, 赵峰. 一株光合细菌的分离鉴定及其产电特性[J]. 环境工程学报, 2014, 8(10): 4503-4507.
引用本文: 吴义诚, 肖勇, 赵峰. 一株光合细菌的分离鉴定及其产电特性[J]. 环境工程学报, 2014, 8(10): 4503-4507.
shu
Wu Yicheng, Xiao Yong, Zhao Feng. Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4503-4507.
Citation: Wu Yicheng, Xiao Yong, Zhao Feng. Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4503-4507.
shu

一株光合细菌的分离鉴定及其产电特性

  • 1.

    中国科学院城市环境研究所, 厦门 361021

  • 基金项目:

    国家“863”高技术研究发展计划项目（2011AA060907）

  • 中图分类号: X172

Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics

  • 1.

    Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

  • Fund Project:

  • 摘要: 采用光照富集及厌氧划线法从污水厂二沉池活性污泥分离到一株光合细菌YC-1，结合菌落特征、细胞形态、活细胞吸收光谱及16S rRNA基因序列分析等对其进行了分类学鉴定，并研究了YC-1在不同光源照射下的产电性能。结果表明，该菌为短杆状，有鞭毛，菌落呈淡粉色，含有大量细菌叶绿素a，16S rRNA基因序列与Rhodopseudomonas palustris DX-1相似度为99%，属于假单胞菌属（Pseudomonas sp.）；双室微生物燃料电池的阳极室接种YC-1，并以乙酸钠为底物，铁氰化钾作为阴极电子受体，外载为1 000 Ω时，电池稳定运行时输出电压为0.58 V，且输出电压不受光源光谱影响。
    Abstract: A photosynthetic bacterium strain YC-1 was isolated from the activate sludge of a wastewater treatment plant through light enrichment and anaerobic separation.The YC-1 was identified as Rhodopseudomonas sp.according to morphological properties of colony and cell,absorption spectrum of living cells and the 16S rRNA gene sequence.The stable voltage output of the microbial fuel cell (MFC) with YC-1 as anodic inoculum reached 0.58 V with an external resistance of 1 000 Ω.The electricity generation of MFC inoculated by YC-1 in anode chamber was not affected by light sources.
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  • 收稿日期:  2013-10-12
  • 刊出日期:  2014-09-28
吴义诚, 肖勇, 赵峰. 一株光合细菌的分离鉴定及其产电特性[J]. 环境工程学报, 2014, 8(10): 4503-4507.
引用本文: 吴义诚, 肖勇, 赵峰. 一株光合细菌的分离鉴定及其产电特性[J]. 环境工程学报, 2014, 8(10): 4503-4507.
shu
Wu Yicheng, Xiao Yong, Zhao Feng. Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4503-4507.
Citation: Wu Yicheng, Xiao Yong, Zhao Feng. Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4503-4507.
shu

一株光合细菌的分离鉴定及其产电特性

  • 1. 中国科学院城市环境研究所, 厦门 361021
  • 收稿日期:  2013-10-12
基金项目:

国家“863”高技术研究发展计划项目（2011AA060907）

摘要: 采用光照富集及厌氧划线法从污水厂二沉池活性污泥分离到一株光合细菌YC-1，结合菌落特征、细胞形态、活细胞吸收光谱及16S rRNA基因序列分析等对其进行了分类学鉴定，并研究了YC-1在不同光源照射下的产电性能。结果表明，该菌为短杆状，有鞭毛，菌落呈淡粉色，含有大量细菌叶绿素a，16S rRNA基因序列与Rhodopseudomonas palustris DX-1相似度为99%，属于假单胞菌属（Pseudomonas sp.）；双室微生物燃料电池的阳极室接种YC-1，并以乙酸钠为底物，铁氰化钾作为阴极电子受体，外载为1 000 Ω时，电池稳定运行时输出电压为0.58 V，且输出电压不受光源光谱影响。

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