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有机负荷冲击对固定床厌氧反应器启动及古菌群落动态影响

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赵洪颜, 于海茹, 陈迪, 朴仁哲, 李虎林, 崔宗均. 有机负荷冲击对固定床厌氧反应器启动及古菌群落动态影响[J]. 环境工程学报, 2015, 9(10): 4655-4663. doi: 10.12030/j.cjee.20151008
引用本文: 赵洪颜, 于海茹, 陈迪, 朴仁哲, 李虎林, 崔宗均. 有机负荷冲击对固定床厌氧反应器启动及古菌群落动态影响[J]. 环境工程学报, 2015, 9(10): 4655-4663. doi: 10.12030/j.cjee.20151008
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Zhao Hongyan, Yu Hairu, Chen Di, Piao Renzhe, Li Hulin, Cui Zongjun. Impact of organic loading rate shock on operation and archaea communities dynamics in different anaerobic fixed-bed reactors[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4655-4663. doi: 10.12030/j.cjee.20151008
Citation: Zhao Hongyan, Yu Hairu, Chen Di, Piao Renzhe, Li Hulin, Cui Zongjun. Impact of organic loading rate shock on operation and archaea communities dynamics in different anaerobic fixed-bed reactors[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4655-4663. doi: 10.12030/j.cjee.20151008
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有机负荷冲击对固定床厌氧反应器启动及古菌群落动态影响

  • 1.

    延边大学, 延吉 133002

  • 2.

    延边州农科院, 延吉 133002

  • 3.

    中国农业大学农学与生物技术学院/中国农业大学生物质工程中心, 北京 100193

  • 基金项目:

    公益性行业(农业)科研专项(201303080)

    国家"863"高技术研究发展计划资助项目(2012BAD14B01)

  • 中图分类号: X703.1

Impact of organic loading rate shock on operation and archaea communities dynamics in different anaerobic fixed-bed reactors

  • 1.

    Yanbian University, Yanji 133002, China

  • 2.

    Yanbian Academy of Agricultural Science, Yanji 133002, China

  • 3.

    College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China

  • Fund Project:

  • 摘要: 为开发高效率、抗冲击能力强的有机废水沼气发酵技术,以人工配制的糖蜜废水为试材,在不同有机负荷(1.7~6.73 kg/(m3·d))的冲击方式下,对4个炭纤维膜为载体固定床厌氧反应器的启动进行了研究。通过COD去除率、pH、产气量、甲烷含量、变性梯度凝胶电泳(DGGE)、16S rDNA克隆文库技术、实时荧光定量PCR(Q-PCR)等指标和方法,分析炭纤维载体在抵御有机负荷冲击过程中的作用及对微生物定殖的贡献。结果表明,在不同有机负荷冲击下,4个炭纤维载体固定床厌氧反应器在第40天时均启动成功,其产气量稳定在21 L 左右,出水pH在6.8~7.5之间,COD去除率在80%以上,甲烷含量在75%以上,其中冲击最大的R2反应器(COD每隔5 d增加5 000 mg/L)的有机负荷是固定床厌氧反应器的一个阈值。附着在炭纤维载体上的产甲烷古菌的多样性丰富,并且繁殖缓慢、容易附着的产甲烷微菌(MMB)的16S rRNA基因浓度最高,反应器中占优势的产甲烷菌是甲烷鬃毛菌(MST)和产甲烷微菌(MMB),说明炭纤维载体对微生物的定殖起到非常重要的作用,提高了污泥活性,从而提高了反应器的性能。
    Abstract: To develop a biogas fermentation technology with high efficiency and resistance of shock loading,in this study,the simulated molasses wastewater was used as material and the impact of different organic loading(1.7—6.73 kg/(m3·d)) on fixed-bed reactors packed with active carbon fiber was investigated.Chemical oxygen demand (COD) removal rate,pH,biogas production,methane content,denaturing gradient gel electrophoresis(DGGE),16S rRNA gene clone library screening,and quantitative PCR et al.were used to analyze the role and contribution of carbon fiber carrier to the colonization of microbial community in the process of resisting the organic load shock.Under different organic loading shocks,four fixed-bed reactors were all started-up successively on the 40th day,and biogas production remained stable at 21 L,effluent pH remained between 6.8—7.5,and COD removal efficiency and the biogas methane content were over 80% and 75%,respectively.The OLR of R2 (COD 5 d increased 5 000 mg/L) was the operational threshold of the fixed-bed anaerobic reactor.Findings revealed that methanogenic archaea were typically dominant in the adhering sludge.Methanomicrobiales which were breeding slowly and attached to the carrier easily were identified in carbon fiber carriers.The 16S rRNA gene concentration of methanogenic archaea was higher in the adhering sludge.The methanosaetaceae and methanomicrobiales were the dominant methanogense in fixed-bed reactors packed with active carbon fiber.Our results indicated that the carbon fiber carriers played a very important role on the colonization of the microorganism,as well as on the improvement of sludge activity and the ability of the reactor to resist shock loading.
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  • [1] 成官文,章非娟,穆军,等.糖蜜酒精废液厌氧生物处理研究进展.环境科学与技术,2003,26(1):50-52Cheng Guanwen,Zhang Feijuan,Mu Jun,et al.Research progress of molasses alcohol wastewater with anaerobic treatment.Environmental Science & Technology,2003,26(1):50-52
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  • 收稿日期:  2014-09-24
  • 刊出日期:  2015-10-14

有机负荷冲击对固定床厌氧反应器启动及古菌群落动态影响

  • 1. 延边大学, 延吉 133002
  • 2. 延边州农科院, 延吉 133002
  • 3. 中国农业大学农学与生物技术学院/中国农业大学生物质工程中心, 北京 100193
  • 收稿日期:  2014-09-24
基金项目:

公益性行业(农业)科研专项(201303080)

国家"863"高技术研究发展计划资助项目(2012BAD14B01)

摘要: 为开发高效率、抗冲击能力强的有机废水沼气发酵技术,以人工配制的糖蜜废水为试材,在不同有机负荷(1.7~6.73 kg/(m3·d))的冲击方式下,对4个炭纤维膜为载体固定床厌氧反应器的启动进行了研究。通过COD去除率、pH、产气量、甲烷含量、变性梯度凝胶电泳(DGGE)、16S rDNA克隆文库技术、实时荧光定量PCR(Q-PCR)等指标和方法,分析炭纤维载体在抵御有机负荷冲击过程中的作用及对微生物定殖的贡献。结果表明,在不同有机负荷冲击下,4个炭纤维载体固定床厌氧反应器在第40天时均启动成功,其产气量稳定在21 L 左右,出水pH在6.8~7.5之间,COD去除率在80%以上,甲烷含量在75%以上,其中冲击最大的R2反应器(COD每隔5 d增加5 000 mg/L)的有机负荷是固定床厌氧反应器的一个阈值。附着在炭纤维载体上的产甲烷古菌的多样性丰富,并且繁殖缓慢、容易附着的产甲烷微菌(MMB)的16S rRNA基因浓度最高,反应器中占优势的产甲烷菌是甲烷鬃毛菌(MST)和产甲烷微菌(MMB),说明炭纤维载体对微生物的定殖起到非常重要的作用,提高了污泥活性,从而提高了反应器的性能。

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