高级搜索

喹诺酮抗性基因在城市污水处理系统中的分布及去除

,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
引用本文: 李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu
Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
Citation: Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu

喹诺酮抗性基因在城市污水处理系统中的分布及去除

  • 1.

    石河子大学化学化工学院／新疆兵团化工绿色过程重点实验室, 石河子 832000

  • 基金项目:

    国家自然科学基金资助项目(21267019)

  • 中图分类号: X703

Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant

  • 1.

    Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China

  • Fund Project:

  • 摘要: 城市污水处理厂是耐药细菌和抗生素抗性基因进入自然环境的主要途径之一。对污水处理厂不同工艺采集7组样品,重点考察污水处理系统中喹诺酮类耐药细菌和7种喹诺酮类抗性基因污染分布及其去除效率。结果显示,在不同处理工艺阶段均检测到耐环丙沙星细菌,最高浓度为(6.65±0.19)×106 CFU/mL,耐药率为0.87%~7.24%。污水处理系统受抗生素抗性基因严重污染,入水样品中抗性基因总浓度为(1.28±0.12)×107 copies/mL。污水处理工艺对抗环丙沙星细菌和喹诺酮类抗性基因具有有效的去除作用,除qnrC去除效率为75%,其余均在98%以上,但是仍有浓度为2.77×104 CFU/mL的耐环丙沙星细菌和3.98×104 copies/mL的抗性基因进入自然环境。表明污水处理系统已成为耐药细菌和抗生素抗性基因的储存库和污染源。
    Abstract: The municipal sewage treatment plant is a major route through which antibiotic bacteria as well as antibiotic-resistance genes from anthropogenic sources are introduced into the natural ecosystems. In the present study, wastewater samples were collected from a sequence of process stages in the municipal sewage treatment plant. The concentrations of quinolone-resistant bacteria and quinolone-resistance genes were investigated, and their removal efficiency was presented in a preliminary analysis. Ciprofloxacin-resistant bacteria were detected in all treatment processes of the sewage treatment plant, and the highest concentration was found to be (6.65±0.19)×106 CFU/mL in the influent. The rates of ciprofloxacin-resistant bacteria made up 0.87% to 7.24% of total bacteria. It was observed that quinolone-resistance genes were prevalent in all the samples from the sewage treatment plant, except for the qnrB gene. The total concentration of the quinolone-resistance genes was (1.28±0.12)×107 copies/mL in the influent from the sewage treatment processes. The ciprofloxacin-resistant bacteria and quinolone-resistance genes were effectively removed during the sewage treatment processes, and their removal efficiency was more than 98%, except for the qnrC gene. However, a high abundance of ciprofloxacin-esistant bacteria and quinolone-resistance genes were released in the natural environment. This indicates that the sewage treatment systems have become reservoirs and contamination sources for the antibiotic-resistant bacteria and antibiotic-resistance genes.
  • 加载中
  • [1] 徐冰洁, 罗义, 周启星, 等. 抗生素抗性基因在环境中的来源、传播扩散及生态风险. 环境化学, 2010, 29(2):169-178 Xu Bingjie, Luo Yi, Zhou Qixing, et al. Sources, dissemination, and ecolagical risks of antibiotic resistances genes (AkGs) in the environment. Environmental Chemistry, 2010, 29(2):169-178(in Chinese)
    [2] 文汉卿, 史俊, 寻昊, 等. 抗生素抗性基因在水环境中的分布、传播扩散与去除研究进展. 应用生态学报, 2015, 26(2):625-635 Wen Hanqing, Shi Jun, Xun Hao, et al. Distribution, dissemination and removal of antibiotic resistant genes (ARGs) in the aquatic environment. Chinese Journal of Applied Ecology, 2015, 26(2):625-635(in Chinese)
    [3] Pruden A., Pei Ruoting, Storteboom H., et al. Antibiotic resistance genes as emerging contaminants:Studies in northern Colorado. Environmental Science & Technology, 2006, 40(23):7445-7450
    [4] Su Haochang, Ying Guangguo, Tao Ran, et al. Class 1 and 2 integrons, sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River, South China. Environmental Pollution, 2012, 169:42-49
    [5] Ji Xiuling, Shen Qunhui, Liu Fang, et al. Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai, China. Journal of Hazardous Materials, 2012, 235-236:178-185
    [6] Nõlvak H., Truu M., Tiirik K., et al. Dynamics of antibiotic resistance genes and their relationships with system treatment efficiency in a horizontal subsurface flow constructed wetland. Science of the Total Environment, 2013, 461-462:636-644
    [7] 张月琴. 氟喹诺酮类药物的药理特点及研究概. 临床和实验医学杂志, 2013, 12(24):2024-2027
    [8] 佟娟, 魏源送. 污水处理厂削减耐药菌与抗性基因的研究进展. 环境科学学报, 2012, 32(11):2650-2659 Tong Juan, Wei Yuansong. State-of-the-art removal of antibiotic resistance bacteria (ARB) and antibiotic resistance gene (ARG) in wastewater treatment plants (WWTPs). Acta Scientiae Circumstantiae, 2012, 32(11):2650-2659(in Chinese)
    [9] Guillard T., Moret H., Brasme L., et al. Rapid detection of qnr and qepA plasmid-mediated quinolone resistance genes using real-time PCR. Diagnostic Microbiology and Infectious Disease, 2011, 70(2):253-259
    [10] 窦春玲, 郭雪萍, 尹大强. 污水处理厂抗生素抗性基因分布和去除研究进展. 环境化学, 2013, 32(10):1885-1893 Dou Chunling, Guo Xueping, Yin Daqiang. Review on distribution and removal of antibiotic resistance genes(ARGs) in wastewater treatment plants (WWTPs). Environmental Chemistry, 2013, 32(10):1885-1893(in Chinese)
    [11] Novo A., Manaia C. M. Factors influencing antibiotic resistance burden in municipal wastewater treatment plants. Applied Microbiology and Biotechnology, 2010, 87(3):1157-1166
    [12] Al-Jassim N., Ansari M. I., Harb M., et al. Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia:Is the treated wastewater safe to reuse for agricultural irrigation. Water Research, 2015, 73:277-290
    [13] Brooks J. P., Maxwell S. L., Rensing C., et al. Occurrence of antibiotic-resistant bacteria and endotoxin associated with the land application of biosolids. Canadian Journal of Microbiology, 2007, 53(5):616-622
    [14] Xu Jian, Xu Yan, Wang Hongmei, et al. Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river. Chemosphere, 2015, 119:1379-1385
    [15] Gao Panpan, Mao Daqing, Luo Yi, et al. Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment. Water Research, 2012, 46(7):2355-2364
    [16] Munir M., Wong K., Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan. Water Research, 2011, 45(2):681-693
    [17] Zhuang Yao, Ren Hongqiang, Geng Jinju, et al. Inactivation of antibiotic resistance genes in municipal wastewater by chlorination, ultraviolet, and ozonation disinfection. Environmental Science and Pollution Research, 2015, 22(9):7037-7044
    [18] Li Jianan, Cheng Weixiao, Xu Like, et al. Antibiotic-resistant genes and antibiotic-resistant bacteria in the effluent of urban residential areas, hospitals, and a municipal wastewater treatment plant system. Environmental Science and Pollution Research, 2015, 22(6):4587-4596
    [19] Mckinney C. W., Loftin K. A., Meyer M. T., et al. tet and sul antibiotic resistance genes in livestock lagoons of various operation type, configuration, and antibiotic occurrence. Environmental Science & Technology, 2010, 44(16):6102-6109
    [20] Yuan Qingbin, Guo Meiting, Yang Jian. Monitoring and assessing the impact of wastewater treatment on release of both antibiotic-resistant bacteria and their typical genes in a Chinese municipal wastewater treatment plant. Environmental Science Process & Impacts, 2014, 16(8):1930-1937
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2380
  • HTML全文浏览数:  2027
  • PDF下载数:  524
  • 施引文献:  0
出版历程
  • 收稿日期:  2015-10-14
  • 刊出日期:  2016-03-18
李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
引用本文: 李超, 鲁建江, 童延斌, 刘江, 李超道, 郑晓伍. 喹诺酮抗性基因在城市污水处理系统中的分布及去除[J]. 环境工程学报, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu
Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
Citation: Li Chao, Lu Jianjiang, Tong Yanbin, Liu Jiang, Li Chaodao, Zheng Xiaowu. Removal of quinolone resistance bacteria and corresponding resistance genes in a conventional municipal sewage treatment plant[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1177-1183. doi: 10.12030/j.cjee.20160328
shu

喹诺酮抗性基因在城市污水处理系统中的分布及去除

  • 1. 石河子大学化学化工学院／新疆兵团化工绿色过程重点实验室, 石河子 832000
  • 收稿日期:  2015-10-14
基金项目:

国家自然科学基金资助项目(21267019)

摘要: 城市污水处理厂是耐药细菌和抗生素抗性基因进入自然环境的主要途径之一。对污水处理厂不同工艺采集7组样品,重点考察污水处理系统中喹诺酮类耐药细菌和7种喹诺酮类抗性基因污染分布及其去除效率。结果显示,在不同处理工艺阶段均检测到耐环丙沙星细菌,最高浓度为(6.65±0.19)×106 CFU/mL,耐药率为0.87%~7.24%。污水处理系统受抗生素抗性基因严重污染,入水样品中抗性基因总浓度为(1.28±0.12)×107 copies/mL。污水处理工艺对抗环丙沙星细菌和喹诺酮类抗性基因具有有效的去除作用,除qnrC去除效率为75%,其余均在98%以上,但是仍有浓度为2.77×104 CFU/mL的耐环丙沙星细菌和3.98×104 copies/mL的抗性基因进入自然环境。表明污水处理系统已成为耐药细菌和抗生素抗性基因的储存库和污染源。

English Abstract

    全文HTML

参考文献 (20)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心