溴阻燃剂对肝细胞的毒性

那广水; 孙丽娟; 张雯雯; 王惠国

生态毒理学报

溴阻燃剂对肝细胞的毒性

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那广水, 孙丽娟, 张雯雯, 王惠国. 溴阻燃剂对肝细胞的毒性[J]. 生态毒理学报, 2012, 7(5): 570-573.

引用本文:

那广水, 孙丽娟, 张雯雯, 王惠国. 溴阻燃剂对肝细胞的毒性[J]. 生态毒理学报, 2012, 7(5): 570-573.

Na Guangshui, Sun Lijuan, Zhang Wenwen, Wang Huiguo. Hepatotoxicity of Brominated Flame Retardants[J]. Asian journal of ecotoxicology, 2012, 7(5): 570-573.

Citation:

Na Guangshui, Sun Lijuan, Zhang Wenwen, Wang Huiguo. Hepatotoxicity of Brominated Flame Retardants[J]. Asian journal of ecotoxicology, 2012, 7(5): 570-573.

溴阻燃剂对肝细胞的毒性

1. 国家海洋环境监测中心国家海洋局近岸海域生态环境重点实验室, 大连 116023;
2. 大连大学生命科学与技术学院, 大连 116622

作者简介: 那广水(1977-),男,副研究员,主要研究方向:环境新兴污染物分析与毒理研究,E-mail,gsna@nmemc.gov.cn

基金项目:
海洋公益性科研专项(201105013)
中图分类号: X171.5

Hepatotoxicity of Brominated Flame Retardants

1. Key Laboratory for Ecological Environment in Coast Areas of State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian 116622, China;
2. College of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China
Fund Project:

摘要: 为评价四溴联苯醚(2,2,4,4-tetrabromodiphenyl ether,PBDE-47)、五溴联苯醚(2,2',4,4',5-pentabromodiphenyl ether,PBDE-99)、九溴联苯醚(polybrominated diphenyl ethers 206,PBDE-206)、六溴环十二烷(hexabromocyclododecane,HBCD)、四溴双酚A (tetrabromobisphenol A,TBBPA)对人肝细胞(LO₂)的体外细胞毒性。将5种溴阻燃剂与人肝细胞LO₂共同培养,采用四甲基偶氮噻唑蓝比色法(MTT法)检测5种溴阻燃剂对LO₂细胞的相对增殖率,并按照GB/T16886.5-2003/ISO10993-5:1999标准评价5种溴阻燃剂对LO₂的细胞毒性。体外实验表明,5种溴阻燃剂对LO₂细胞均具有抑制作用,其中PBDE-47、PBDE-99和HBCD呈现剂量依赖关系。
- 四溴联苯醚 /
- 五溴联苯醚 /
- 九溴联苯醚 /
- 六溴环十二烷 /
- 四溴双酚A /
- LO₂细胞
Abstract: To test the toxic effects of 2, 2, 4, 4-tetrabromodiphenyl ether (PBDE-47), 2,2',4,4',5-pentabromodiphenyl ether (PBDE-99), polybrominated diphenyl ethers 206 (PBDE-206), hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) on normal human hepatocytes (LO₂) in vitro, the human liver cell line LO₂ was co-cultured with these five brominated flame retardants respectively. The inhibition rate of LO₂ cells was detected by MTT (3-(4,5-Dimethylthia zol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the cytotoxicity was evaluated in accordance with the criterion of GB/T16886.5-2003/ISO10993-5:1999. As a result, these five brominated flame retardants showed inhibiting effect on the LO₂ cells in vitro experiments. PBDE-47, PBDE-99 and HBCD showed dose-response relation.

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那广水, 孙丽娟, 张雯雯, 王惠国. 溴阻燃剂对肝细胞的毒性[J]. 生态毒理学报, 2012, 7(5): 570-573.

引用本文:

那广水, 孙丽娟, 张雯雯, 王惠国. 溴阻燃剂对肝细胞的毒性[J]. 生态毒理学报, 2012, 7(5): 570-573.

Na Guangshui, Sun Lijuan, Zhang Wenwen, Wang Huiguo. Hepatotoxicity of Brominated Flame Retardants[J]. Asian journal of ecotoxicology, 2012, 7(5): 570-573.

Citation:

Na Guangshui, Sun Lijuan, Zhang Wenwen, Wang Huiguo. Hepatotoxicity of Brominated Flame Retardants[J]. Asian journal of ecotoxicology, 2012, 7(5): 570-573.

溴阻燃剂对肝细胞的毒性

作者简介: 那广水(1977-),男,副研究员,主要研究方向:环境新兴污染物分析与毒理研究,E-mail,gsna@nmemc.gov.cn
1. 国家海洋环境监测中心国家海洋局近岸海域生态环境重点实验室, 大连 116023;

2. 大连大学生命科学与技术学院, 大连 116622

收稿日期: 2012-07-21

基金项目:

海洋公益性科研专项(201105013)

关键词:

摘要: 为评价四溴联苯醚(2,2,4,4-tetrabromodiphenyl ether,PBDE-47)、五溴联苯醚(2,2',4,4',5-pentabromodiphenyl ether,PBDE-99)、九溴联苯醚(polybrominated diphenyl ethers 206,PBDE-206)、六溴环十二烷(hexabromocyclododecane,HBCD)、四溴双酚A (tetrabromobisphenol A,TBBPA)对人肝细胞(LO₂)的体外细胞毒性。将5种溴阻燃剂与人肝细胞LO₂共同培养,采用四甲基偶氮噻唑蓝比色法(MTT法)检测5种溴阻燃剂对LO₂细胞的相对增殖率,并按照GB/T16886.5-2003/ISO10993-5:1999标准评价5种溴阻燃剂对LO₂的细胞毒性。体外实验表明,5种溴阻燃剂对LO₂细胞均具有抑制作用,其中PBDE-47、PBDE-99和HBCD呈现剂量依赖关系。

English Abstract

Hepatotoxicity of Brominated Flame Retardants

1. Key Laboratory for Ecological Environment in Coast Areas of State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian 116622, China;
2. College of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China

Received Date: 2012-07-21

Fund Project:

Keywords:

Abstract: To test the toxic effects of 2, 2, 4, 4-tetrabromodiphenyl ether (PBDE-47), 2,2',4,4',5-pentabromodiphenyl ether (PBDE-99), polybrominated diphenyl ethers 206 (PBDE-206), hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) on normal human hepatocytes (LO₂) in vitro, the human liver cell line LO₂ was co-cultured with these five brominated flame retardants respectively. The inhibition rate of LO₂ cells was detected by MTT (3-(4,5-Dimethylthia zol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the cytotoxicity was evaluated in accordance with the criterion of GB/T16886.5-2003/ISO10993-5:1999. As a result, these five brominated flame retardants showed inhibiting effect on the LO₂ cells in vitro experiments. PBDE-47, PBDE-99 and HBCD showed dose-response relation.