施氏假单胞菌N2代谢邻/间/对甲酚的特性研究
Characterization of o-/m-/p-cresol Metabolizated by Pseudomonas stutzeri N2
-
摘要: 本论文以施氏假单胞菌N2为受试菌株，研究了N2菌对邻/间/对甲酚及其混合物的生物降解特性.结果表明，N2菌能以邻/间/对甲酚为唯一碳源和能源生长，但对3种异构体的降解速率各异.完全降解600 mg·L-1的对甲酚仅需6 h，间甲酚则需24 h，但对邻甲酚的降解明显减缓；200 mg·L-1邻甲酚48 h的降解率仅为11.38%.GC-MS结果分析发现，N2菌代谢甲酚途径主要为甲基氧化、芳环羟化，随后脱羧、开环裂解、降解转化至矿化，但3种甲酚的降解途径及酸性代谢产物的形成次序不一致.3种甲酚混合存在时可促进N2菌对其降解，这主要是因为混合碳源的协同作用减少了体系中因产酸过多引起的毒性，从而促进了N2菌对甲酚的降解矿化.Abstract: In this paper,the biodegradation of o-/m-/p-cresol and their mixture by Pseudomonas stutzeri N2 was studied. The results showed that the strain N2 was able to grow with each isomer of o-/m-/p-cresol as the sole carbon source and energy,and the degradation rate of the each isomer of o,m,p-cresol was obviously different. When the concentration of cresol was 600 mg·L-1,the complete degradation of p-cresol only needed 6 h,and m-cresol needed 24 h by strain N2. But the degradation of o-cresol was significantly slow,the degradation rate of 200 mg·L-1 o-cresol for 48 h was only 11.38%. The analysis of GC-MS found that the metabolic pathways of cresols by the strain N2 were mainly including:methyl oxidation,aromatic hydroxylation,and afterward decarboxylation,ring opening degradation,conversion even to mineralization. The degradation pathways and the gendrating orders of the acid intermediates of the o-/m-/p-cresol were diverse from each other. With the cresols of same concentration mixed,the degradation of cresols by Pseudomonas stutzeri N2 was promoted,mainly because the synergistic action had reduced the toxicity caused by excessive acid products in the system,thus promoting the degradation and mineralization of cresols.
-
Key words:
- Pseudomonas stutzeri N2 /
- cresol /
- biodegradation /
- synergistic action
-
-
[1] DUAN W Y, MENG F P,CUI H W,et al.,Ecotoxicity of phenol and cresols to aquatic organisms:A review[J].Ecotoxicology and Environmental Safety,2018,157:441-456. [2] 张万辉,韦朝海,晏波,等.焦化废水中溶解性有机物组分的特征分析[J].环境化学,2012,31(5):702-707. ZHANG W H,WEI C H,YAN B,et.al.Characteristic analysis of dissolved organic matter components in coking wastewater[J]. Environmenta Chemistry,2012,31(5):702-707(in Chinese).
[3] AFRIDA W, LU Y, SUN S, et al.,Effect of operational strategies on activated sludge's acclimation to phenol, subsequent aerobic granulation, and accumulation of polyhydoxyalkanoates[J].J Hazard Mater,2016,317:221-228. [4] LIU Y J,ZHANG A N,WANG X C. Biodegradation of phenol by using free and immobilized cells of Acinetobacter sp. XA05 and Sphingomonas sp. FG03.[J].Biochemical Engineering Journal, 2009,44(2-3):187-192. [5] 张云鸽,梅荣武,张宇,等.高盐含酚废水生物处理技术的研究进展及应用前景[J].环境工程,2018,36(5):12-16. ZHANG Y G,MEI R W,ZHANG Y,et al.Research progress of biological treatment technologies for phenolic wastewater with high salinity and their application prospects[J]. Environmental Engineering,2018,36(5):12-16(in Chinese).
[6] STEPHEN D P,AYALUR K B,et al. Phycoremediation of phenolic effluent of a coal gasification plant by chlorella pyrenoidosa[J].Process Satety and Environmental Protection,2017, 111:31-39. [7] 国家环保局《污水综合排放标准》(GB8978-1996)编委会.GB89782-1996 Z60.污水综合排放标准(GB8978-1996)[S].北京:中国环境科学出版社,1997. National Environmental Protection Agency《Integrated Wastewater Discharge Standard》(GB8978-1996) Editorial Board. GB89782 -1996 Z60. Integrated Wastewater Discharge Standard (GB8978-1996)[S]. Beijing:China Environmental Science Press, 1997.
[8] [9] 刘蛟,贾晓强,闻建平.双菌种协同代谢酚类化合物的机制研究[J].环境科学,2011,32(10).3053-3058. LIU J,JIA X Q,WEN J P,et al.Co-metabolism of dual culture in the degradation of phenolic compounds[J].Environmental Science,2011,32(10). 3053-3058(in Chinese).
[10] 石高科,王孟,贾子龙.一株假单胞菌降解间甲酚的特性研究[J].山西建筑,2016,42(24):127-130. SHI G K,WANG M,JIA Z L.Study on the degradation of m-cresol by a strain of Pseudomonas[J].Shanxi Architecture,2016,42(24):127-130(in Chinese).
[11] 彭丽花,任源,邓留杰.间甲酚降解菌Citrobacter farmeri 的降解特性及代谢途径解析[J].环境化学,2009,28(1):44-48. PENG L H,REN Y,DENG L J.Degradation characteristics and metabolic pathways of m-cresol degrading bacteria Citrobacter farmeri[J]. Environmental Chemistry,2009,28(1):44-48(in Chinese).
[12] SHAHAB S,HOSSEIN S Z,KAMAHLDIN H,et al.High phenol degradation capacity of a newly characterized Acinetobacter sp. SA01:Bacterial cell viability and membrane impairment in respect to the phenol toxicity[J].Ecotoxicology and Environmental Safet,.2018,164:455-466. [13] OSCAR F,FRANCISCA R,Rolando C.Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula[J].Electronic Journal of Biotechnology, 2018, 35:33-38. [14] JIANG Y,CAI X.WU D,et al.Biodegradation of phenol and m-cresol by mutated Candida tropicalis[J].Journal of Environmental Sciences,2010, 22(4) 621-626. [15] REN Y,LI H P, ZHAO G B,et al. Degradation of m-cresol via the ortho cleavage pathway by Citrobacter farmeri SC01[J]. Biochemical Engineering Journal, 2014, 88:108-114. [16] PICHIAH S,KANNAN P, PRABIRKUMAR S, et al. Kinetics of phenol and m-cresol biodegradation by an indigenous mixed microbial culture isolated from a sewage treatment plant[J]. Journal of Environmental Sciences, 2008,20:1508-1513. [17] RIHAM S,MUFTAH H, EL-Naas,et al.Biological treatment of wastewater contaminated with p-cresol using Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel[J].Journal of Water Process Engineering,2014,1:84-90. [18] 樊瑜,聂麦茜,葛碧洲,等.耐高浓度苯酚菌株的筛选及其降解特性研究[J].安全与环境学报,2012,12(4):113-117. FAN Y,NIE M Q,GE B Z,et al.Screening of high-phenol-tolerance strain and studying of its phenol-degrading abilities[J].Journal of Safety and Environment,2012,12(4):113-117(in Chinese).
[19] NIE H Y,NIE M Q,YANG Y Z,et al. Characterization of phenol metabolization by P. stutzeri N2[J].Polycyclic Aromatic Compounds,2017,586-599. [20] 陈军.苯酚生物降解开环裂解方式影响因素及其对矿化效率的促进作用[D].西安:西安建筑科技大学, 2017. CHEN J.Influencing factors of phenol biodegradation and ring opening cracking and its promoting effect on mineralization efficiency[D]. Xi'an:Xi'an University of Architecture and Technology,2017(in Chinese). [21] 张金丽,郑天凌.多环芳烃污染环境的控制与生物修复研究进展[J].福建环境,2002,19(2):26-29. ZHANG J L,ZHENG T J.Advances in research on control and bioremediation of polycyclic aromatic hydrocarbons[J].Fujian Environment,2002,19(2):26-29.
[22] 姚华群.液相色谱法测定工业废水中苯酚[J].石化技术与应用,1998,12:240-242. YAO H Q.Determination of phenol in industrial wastewater by liquid chromatography[J].Petrochemical Technology and Application,1998 ,12:240-242(in Chinese).
[23] 崔鹏,马晶,孙谦,等.GC-MS/MS法测定马铃薯中多农药残留含量[J].环境化学,2018,37(9):2087-2090. CUI P,MA J,SUN Q,et al.Determination of pesticide residues in potato by GC-MS/MS[J].Environmental Chemistry,2018,37(9):2087-2090(in Chinese).
[24] 王迪,马晶,李灏,等.GC-MS/MS法测定土壤中16种多环芳烃[J].环境化学,2018,37(3):617-620. WANG D,MA J,LI H,et al.Determination of 16 polycyclic aromatic hydrocarbons in soil by GC-MS/MS method[J].Environmental Chemistry, 2018,37(3):617-620(in Chinese).
[25] CAI W,LI J,ZHANG Z. The characteristics and mechanisms of phenol biodegradation by Fusarium sp[J]. Journal of Hazardous Materials, 2007, 148(1-2):38-42. [26] RATTABAL K,NURAK G. Mechanism pathway and kinetics of p-cresol photocatalytic degradation over titania nanorods under UV-visible irradiation[J].Chemical Engineering Journal,2016, 296:420-427. [27] YAMIN W, HUANGZHAO W,ZHAO Y,et al.The optimization, kinetics and mechanism of m-cresol degradation via catalytic wet peroxide oxidation with sludge-derived carbon catalyst[J].Journal of Hazardous Materials, 2017,326:36-46. -
点击查看大图
计量
- 文章访问数: 1488
- HTML全文浏览数: 1488
- PDF下载数: 55
- 施引文献: 0
下载:
