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高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃

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张磊, 方波, 王宏伟, 杨泽, 李娜, 赵玉琦, 郝玉兰, 王曼曼, 王茜. 高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃[J]. 环境化学, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
引用本文: 张磊, 方波, 王宏伟, 杨泽, 李娜, 赵玉琦, 郝玉兰, 王曼曼, 王茜. 高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃[J]. 环境化学, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
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ZHANG Lei, FANG Bo, WANG Hongwei, YANG Ze, LI Na, ZHAO Yuqi, HAO Yulan, WANG Manman, WANG Qian. Simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons in urine of healthy people by high performance liquid chromatography-fluorescence detection[J]. Environmental Chemistry, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
Citation: ZHANG Lei, FANG Bo, WANG Hongwei, YANG Ze, LI Na, ZHAO Yuqi, HAO Yulan, WANG Manman, WANG Qian. Simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons in urine of healthy people by high performance liquid chromatography-fluorescence detection[J]. Environmental Chemistry, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
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高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃

  • 华北理工大学公共卫生学院, 唐山, 063210

    • 通讯作者: 王茜, E-mail: wwqq517@aliyun.com
  • 基金项目:

    河北省自然科学基金（H2017209232）和河北省卫生计生委项目（20170893）资助.

Simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons in urine of healthy people by high performance liquid chromatography-fluorescence detection

  • School of Public Health, North China University of Science and Technology, Tangshan, 063210, China

    • Corresponding author: WANG Qian, wwqq517@aliyun.com
  • Fund Project: Supported by the Natural Science Foundation of Hebei Province, China (H2017209232) and the Research Foundation of Health and Family Planning Commission of Hebei (20170893).

  • 摘要: 基于固相萃取-高效液相色谱-荧光检测法（SPE-HPLC-FLD），建立了同时测定健康人群尿液中7种羟基多环芳烃（OH-PAHs）的分析方法.10 mL酶解后的尿液样品经固相萃取净化富集.实验中考察了淋洗液、洗脱液和洗脱体积对萃取效率的影响.在最优条件下，7种OH-PAHs在各自范围内线性关系良好且相关系数（r）大于0.9970.方法检出限（S/N=3）和定量限（S/N=10）分别为0.04—4.96 ng·mL-1和0.10—15 ng·mL-1，日内（n=3）和日间（n=3）相对标准偏差分别为1.4%—8.9%和3.2%—11.5%.用该方法对40份健康人群尿液样品进行测定，OH-PAHs检出率为20.0%—100.0%，平均回收率在88.5%—102.7%之间.该方法操作简单、灵敏、准确，可应用于尿液中羟基多环芳烃的测定.
    Abstract: A method based on C18 solid phase extraction (SPE) coupled with high performance liquid chromatography-fluorescence detection (SPE-HPLC-FLD) was developed for the simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) in urine of healthy people. The 10 mL enzymatic hydrolyzed urine sample was purified and enriched by SPE process. The parameters influenced on SPE extraction efficiency such as the washing solvent, eluting solvent and eluting volume were investigated in detail. Under the optimized conditions, the method gave good linear ranges with the correlation coefficients (r) higher than 0.9970. The detection limits (S/N=3) and the quantification limits (S/N=10) were in the ranges of 0.04-4.96 ng·mL-1 and 0.10-15.00 ng·mL-1, respectively. The relative standard deviations of intra-day (n=3) and inter-day (n=3) were 1.4%-8.9% and 3.2%-11.5%, respectively. The developed method was applied to monitor the OH-PAHs in urine samples of 40 healthy people. The detection rate of seven target OH-PAHs was 20.0%-100.0% and the average recoveries at three spiked levels were 88.5%-102.7%. The method could be applied for the determination of OH-PAHs in urine of healthy people in a simple, sensitive and accurate way.
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  • [1] 马军,滕应,陆引罡,等. 四通道色谱分离仪净化/气相色谱-质谱法测定烟叶中的多环芳烃[J]. 分析测试学报,2016,35(8):968-973.

    MA J, TENG Y, LU Y G, et al. Determination of 16 polycyclic aromatic hydrocarbons in tobacco (Nicotiana tabacum L) by four-channel chromatograph purification coupled with gas chromatography-mass spectrometry[J]. Journal of Instrumental Analysis, 2016, 35(8):968-973(in Chinese).

    [2] 宋喜丽,胡小键,林少彬. 人尿中羟基多环芳烃分析方法的研究进展[J]. 环境与健康杂志,2017,34(6):555-560.

    SONG X L, HU X J, LIN S B. Analytical methods of hydroxyl polycyclic aromatic hydrocarbons in human urine:A review of recent studies[J]. Journal of Environment and Health, 2017, 34(6):555-560(in Chinese).

    [3] JONGENEELEN F J. Benchmark guideline for urinary 1-hydroxypyrene as biomarker of occupational exposure to polycyclic aromatic hydrocarbons[J]. Annals of Occupational Hygiene, 2001, 45(1):3-13.
    [4] 谭振,陆少游,吴宏海,等. 非职业暴露居民多环芳烃内暴露水平调查[J]. 环境与健康杂志,2013,30(9):830-831.

    TAN Z, LU S Y, WU H H, et al. Investigation on the exposure level of polycyclic aromatic hydrocarbons in non-occupational exposed residents[J]. Journal of Environment and Health, 2013, 30(9):830-831(in Chinese).

    [5] LI Z, ROMANOFF L C, TRINIDAD D A, et al. Measurement of urinary monohydroxy polycyclic aromatic hydrocarbons using automated liquid-liquid extraction and gas chromatography/isotope dilution high-resolution mass spectrometry[J]. Analytical Chemistry, 2006, 78(16):5744-5751.
    [6] 孙谦,王立闱,范军,等. GC-MS/MS测定生活饮用水中16种多环芳烃[J]. 环境化学, 2016,35(3):604-606.

    SUN Q, WANG L W, FAN J, et al. Determination of 16 polycyclic aromatic hydrocarbons in drinking water by GC-MS/MS[J]. Environmental Chemistry, 2016, 35(3):604-606(in Chinese).

    [7] FERRARI S, MANDEL F, BERSET J D. Quantitative determination of 1-hydroxypyrene in bovine urine samples using high-performance liquid chromatography with fluorescence and mass spectrometric detection[J]. Chemosphere, 2002, 47(2):173-182.
    [8] WIELE T R V D, PERU K M, VERSTRAETE W, et al. Liquid chromatography-mass spectrometry analysis of hydroxylated polycyclic aromatic hydrocarbons, formed in a simulator of the human gastrointestinal tract[J]. Journal of Chromatography B, 2004, 806(2):245-253.
    [9] ZHAO G, CHEN Y, WANG S, et al. Simultaneous determination of 11 monohydroxylated PAHs in human urine by stir bar sorptive extraction and liquid chromatography/tandem mass spectrometry[J]. Talanta, 2013, 116:822-826.
    [10] 李永新,黎源倩,张彩云. 尿中4种多环芳烃代谢产物的高效液相色谱-荧光检测方法研究[J]. 现代预防医学,2011,38(22):4706-4708.

    LI Y X, LI Y Q, ZHANG C Y. Simultaneous determination of four metabolites of polycyclic aromatic hydrocarbons in urine by high performance liquid chromatography with fluorescence detection[J]. Modern Preventive Medicine, 2011, 38(22):4706-4708(in Chinese).

    [11] 胡桂羽,王曼曼,念琪循,等. 基于整体柱的固相萃取-高效液相色谱法测定尿液中4种羟基多环芳烃[J]. 色谱,2018,36(4):370-375.

    HU G Y, WANG M M, NIAN Q X, et al. Determination of four hydroxyl polycyclic aromatic hydrocarbons In urine by solid phase extraction with monolithic column coupled with high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2018, 36(4):370-375(in Chinese).

    [12] FAN R, RAMAGE R, WANG D, Et al. Determination of ten monohydroxylated polycyclic aromatic hydrocarbons by liquid-liquid extraction and liquid chromatography/tandem mass spectrometry[J]. Talanta, 2012, 93:383-391.
    [13] LI Z, ROMANOFF L C, TRINIDAD D A, et al. Quantification of 21 metabolites of methylnaphthalenes and polycyclic aromatic hydrocarbons in human urine[J]. Analytical and Bioanalytical Chemistry, 2014, 406(13):3119-3129.
    [14] 成琳,李金玉,吕胜杰,等超高效液相色谱-串联质谱检测尿中多环芳烃羟基代谢物[J]. 环境与职业医学,2017,34(11):1004-1008.

    CHENG L, LI J Y, LV S J, et al. Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine by ultrasensitive performance liquid chromatography-tandem mass spectrometry[J]. Journal of Environmental & Occupational Medicine, 2017, 34(11):1004-1008(in Chinese).

    [15] 付慧,胡小键,陈曦,等. 超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物[J]. 色谱,2018,36(5):487-492.

    FU H, HU X J, CHEN X, et al. Simultaneous determination of 12 monohydroxylated polycyclicaromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(5):487-492(in Chinese).

    [16] GUPTA M K, JAIN R, SINGH P, et al. Determination of urinary PAH Metabolites using DLLME hyphenated to injector port silylation and GC-MS-MS[J]. Journal of Analytical Toxicology, 2015, 39(5):365-373.
    [17] SERRANO M, BARTOLOMÉ M, BRAVO J C, et al. On-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine samples[J]. Talanta, 2017, 166(2):375-382.
    [18] KIM K H, JAHAN S A, KABIR E, et al. A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects[J]. Environment International, 2013, 60:71-80.
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  • 收稿日期:  2018-12-13
张磊, 方波, 王宏伟, 杨泽, 李娜, 赵玉琦, 郝玉兰, 王曼曼, 王茜. 高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃[J]. 环境化学, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
引用本文: 张磊, 方波, 王宏伟, 杨泽, 李娜, 赵玉琦, 郝玉兰, 王曼曼, 王茜. 高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃[J]. 环境化学, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
shu
ZHANG Lei, FANG Bo, WANG Hongwei, YANG Ze, LI Na, ZHAO Yuqi, HAO Yulan, WANG Manman, WANG Qian. Simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons in urine of healthy people by high performance liquid chromatography-fluorescence detection[J]. Environmental Chemistry, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
Citation: ZHANG Lei, FANG Bo, WANG Hongwei, YANG Ze, LI Na, ZHAO Yuqi, HAO Yulan, WANG Manman, WANG Qian. Simultaneous determination of seven hydroxyl polycyclic aromatic hydrocarbons in urine of healthy people by high performance liquid chromatography-fluorescence detection[J]. Environmental Chemistry, 2019, (11): 2435-2442. doi: 10.7524/j.issn.0254-6108.2018121301
shu

高效液相色谱-荧光检测法同时测定健康人群尿液中7种羟基多环芳烃

    通讯作者: 王茜, E-mail: wwqq517@aliyun.com
  • 华北理工大学公共卫生学院, 唐山, 063210
  • 收稿日期:  2018-12-13
基金项目:

河北省自然科学基金（H2017209232）和河北省卫生计生委项目（20170893）资助.

摘要: 基于固相萃取-高效液相色谱-荧光检测法（SPE-HPLC-FLD），建立了同时测定健康人群尿液中7种羟基多环芳烃（OH-PAHs）的分析方法.10 mL酶解后的尿液样品经固相萃取净化富集.实验中考察了淋洗液、洗脱液和洗脱体积对萃取效率的影响.在最优条件下，7种OH-PAHs在各自范围内线性关系良好且相关系数（r）大于0.9970.方法检出限（S/N=3）和定量限（S/N=10）分别为0.04—4.96 ng·mL-1和0.10—15 ng·mL-1，日内（n=3）和日间（n=3）相对标准偏差分别为1.4%—8.9%和3.2%—11.5%.用该方法对40份健康人群尿液样品进行测定，OH-PAHs检出率为20.0%—100.0%，平均回收率在88.5%—102.7%之间.该方法操作简单、灵敏、准确，可应用于尿液中羟基多环芳烃的测定.

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