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典型有机磷酸酯在小鼠组织内的特异性分布

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陈敏, 廖晓亮, 杨春, 宋德浩, 陈智锋, 刘国光, 祁增华, 蔡宗苇. 典型有机磷酸酯在小鼠组织内的特异性分布[J]. 环境化学, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
引用本文: 陈敏, 廖晓亮, 杨春, 宋德浩, 陈智锋, 刘国光, 祁增华, 蔡宗苇. 典型有机磷酸酯在小鼠组织内的特异性分布[J]. 环境化学, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
shu
CHEN Min, LIAO Xiaoliang, YANG Chun, SUN Dehao, CHEN Zhifeng, LIU Guoguang, QI Zenghua, CAI Zongwei. The tissue accumulation and distribution of typical organophosphate flame retardants (OPFRs) in mice[J]. Environmental Chemistry, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
Citation: CHEN Min, LIAO Xiaoliang, YANG Chun, SUN Dehao, CHEN Zhifeng, LIU Guoguang, QI Zenghua, CAI Zongwei. The tissue accumulation and distribution of typical organophosphate flame retardants (OPFRs) in mice[J]. Environmental Chemistry, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
shu

典型有机磷酸酯在小鼠组织内的特异性分布

  • 1. 广东工业大学环境科学与工程学院, 广州, 510006;

  • 2. 香港浸会大学化学系, 环境与生物分析国家重点实验室, 香港, 999077

    • 通讯作者: 祁增华, E-mail: zenghuaqi@gmail.com ;  蔡宗苇, E-mail: zwcai@hkbu.edu.hk
  • 基金项目:

    国家自然科学基金（21806025，91543202）和广东省自然科学基金（2019A1515011294）资助.

The tissue accumulation and distribution of typical organophosphate flame retardants (OPFRs) in mice

  • 1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, China;

  • 2. State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, 999077, China

    • Corresponding authors: QI Zenghua, zenghuaqi@gmail.com ;  CAI Zongwei, zwcai@hkbu.edu.hk
  • Fund Project: Supported by the National Natural Science Foundation of China (21806025, 91543202) and the Natural Science Foundation of Guangdong Province (2019A1515011294).

  • 摘要: 基于气相色谱质谱（GC-MS/MS）联用建立了生物体内典型有机磷酸酯（OPFRs）的高效提取和仪器分析方法，并探讨了其在小鼠组织中的积累分布特征.本研究通过对样品提取、净化和仪器定性定量等环节的条件优化，建立了高效测定机体中典型OPFRs的超声辅助萃取-无乙二胺-N-丙基硅烷（PSA）柱净化-气相色谱质谱分析方法.结果显示，在最优条件下加标样品中氘代磷酸三丁酯TnBP-d27和氘代磷酸三苯酯TPhP-d15的回收率分别为104.86%和102.12%.暴露实验采用昆明（KM）小鼠单次尾静脉注射OPFRs标记物的方式，分时间段取样，获得实际样品.使用本文建立的方法对实际样品进行定量分析，TnBP-d27和TPhP-d15的线性范围在1—200 μg·L-1之间，r2 > 0.99，检出限为0.02—1.08 μg·L-1，定量限为0.06—3.61 μg·L-1；平均回收率为54.04%—130.29%，相对标准偏差（RSDs）为9.58%—18.21%；TnBP-d27主要在脾脏（36 h）和肾脏（24 h）富集，分别为（8.55±1.00） ng·g-1和（20.34±6.60） ng·g-1；TPhP-d15主要在肾脏（12 h）富集，为（29.86±11.90） ng·g-1.
    Abstract: In this study, an optimized method of solid-phase extraction and GC-MS/MS was applied to characterize and analyze the tissue distribution of typical organophosphate flame retardants (OPFRs) in KM mice. The procedures of extraction and purification, as well as chromatographic and mass spectrometric parameters were optimized. An effective method for determination of typical OPFRs in organism by GC-MS/MS with ultrasonic-assisted solvent extraction and non-primary secondary amine (PSA) purification was developed. Under the optimized conditions, our results showed that recoveries of TnBP-d27 and TPhP-d15 were 104.86% and 102.12%, respectively, showing a suitable extraction method. The tissue samples were obtained from mice after tail intravenous injection with different time points. The calibration curves showed good linearity in 1—200 μg·L-1 with correlation coefficients of r2>0.99. The limits of detection were 0.02—1.08 μg·L-1 and the limits of quantification were 0.06—3.61 μg·L-1. The average spiked recoveries were 54.04%—130.29% with relative standard deviation(RSDs)of 9.58%—18.21%. Spleen and kidney were the most obvious tissues for TnBP-d27 bioaccumulation with the concentration of (8.55±1.00) ng·g-1 in spleen after the 36 h exposure, and (20.34±6.60) ng·g-1 in kidney after the 24 h exposure. The highest concentration of TPhP-d15 was (29.86 ±11.90) ng·g-1 in kidney after exposure of 24 h.
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  • 收稿日期:  2019-12-31
陈敏, 廖晓亮, 杨春, 宋德浩, 陈智锋, 刘国光, 祁增华, 蔡宗苇. 典型有机磷酸酯在小鼠组织内的特异性分布[J]. 环境化学, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
引用本文: 陈敏, 廖晓亮, 杨春, 宋德浩, 陈智锋, 刘国光, 祁增华, 蔡宗苇. 典型有机磷酸酯在小鼠组织内的特异性分布[J]. 环境化学, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
shu
CHEN Min, LIAO Xiaoliang, YANG Chun, SUN Dehao, CHEN Zhifeng, LIU Guoguang, QI Zenghua, CAI Zongwei. The tissue accumulation and distribution of typical organophosphate flame retardants (OPFRs) in mice[J]. Environmental Chemistry, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
Citation: CHEN Min, LIAO Xiaoliang, YANG Chun, SUN Dehao, CHEN Zhifeng, LIU Guoguang, QI Zenghua, CAI Zongwei. The tissue accumulation and distribution of typical organophosphate flame retardants (OPFRs) in mice[J]. Environmental Chemistry, 2020, (10): 2627-2636. doi: 10.7524/j.issn.0254-6108.2019123101
shu

典型有机磷酸酯在小鼠组织内的特异性分布

    通讯作者: 祁增华, E-mail: zenghuaqi@gmail.com ;  蔡宗苇, E-mail: zwcai@hkbu.edu.hk
  • 1. 广东工业大学环境科学与工程学院, 广州, 510006;
  • 2. 香港浸会大学化学系, 环境与生物分析国家重点实验室, 香港, 999077
  • 收稿日期:  2019-12-31
基金项目:

国家自然科学基金（21806025，91543202）和广东省自然科学基金（2019A1515011294）资助.

摘要: 基于气相色谱质谱（GC-MS/MS）联用建立了生物体内典型有机磷酸酯（OPFRs）的高效提取和仪器分析方法，并探讨了其在小鼠组织中的积累分布特征.本研究通过对样品提取、净化和仪器定性定量等环节的条件优化，建立了高效测定机体中典型OPFRs的超声辅助萃取-无乙二胺-N-丙基硅烷（PSA）柱净化-气相色谱质谱分析方法.结果显示，在最优条件下加标样品中氘代磷酸三丁酯TnBP-d27和氘代磷酸三苯酯TPhP-d15的回收率分别为104.86%和102.12%.暴露实验采用昆明（KM）小鼠单次尾静脉注射OPFRs标记物的方式，分时间段取样，获得实际样品.使用本文建立的方法对实际样品进行定量分析，TnBP-d27和TPhP-d15的线性范围在1—200 μg·L-1之间，r2 > 0.99，检出限为0.02—1.08 μg·L-1，定量限为0.06—3.61 μg·L-1；平均回收率为54.04%—130.29%，相对标准偏差（RSDs）为9.58%—18.21%；TnBP-d27主要在脾脏（36 h）和肾脏（24 h）富集，分别为（8.55±1.00） ng·g-1和（20.34±6.60） ng·g-1；TPhP-d15主要在肾脏（12 h）富集，为（29.86±11.90） ng·g-1.

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