摘要:
采集北京城区大气可吸入颗粒物中的细颗粒物(PM2.5),用其对人肺腺癌A549细胞染毒,探讨PM2.5对细胞增殖的毒性和诱导细胞凋亡的作用,并且考察了加入不同浓度的红豆越橘提取物和竹叶提取物对其的抗性作用。实验采用MTT法检测细胞增殖作用,采用Annexin V-FITC/PI双染法和流式细胞仪检测细胞凋亡。结果显示:PM2.5浓度< 150 mg·L-1时可刺激A549细胞增殖,而PM2.5浓度≥ 150 mg·L-1时可抑制细胞增殖;竹叶提取物和红豆越橘提取物浓度< 200 mg·L-1范围内没有明显的细胞毒性作用,但具有抗PM2.5细胞毒性的作用;与PM2.5单独作用组比较,浓度为50 mg·L-1和100 mg·L-1的竹叶提取物可明显降低细胞凋亡率(p < 0.05);红豆越橘提取物也能够降低PM2.5诱导的A549细胞凋亡率,且有剂量-效应关系(r=-0.958,p < 0.05);在相同浓度下(50 mg·L-1),竹叶提取物的拮抗保护能力强于红豆越橘提取物。实验结果表明,竹叶提取物和红豆越橘提取物的细胞毒性较低,一定浓度范围内2种提取物对PM2.5诱导的A549细胞凋亡率有拮抗作用。
Abstract:
The mechanism of cell damage induced by fine particulate matter (PM2.5) and the anti-damage effect produced by natural extracts were studied by human lung carcinoma A549 cells as targets. MTT assay was used to measure the cytotoxicity, and apoptotic rate was detected by flow cytometry with Annexin V-FITC/PI double staining method. The results showed that PM2.5 stimulated the proliferation of A549 cells at lower concentration (< 150 mg·L-1), while PM2.5inhibited the proliferation at higher concentration (≥ 150 mg·L-1). No significant cytotoxicity was observed in cells exposed to bamboo leaf extract and lingonberry extract but anti-cytotoxicity effects were shown at concentrations lower than 200 mg·L-1. Compared to the apoptotic rate induced by PM2.5,bamboo leaf extract could significantly decrease the apoptosis at 50 mg·L-1 and 100 mg·L-1 (p < 0.05). Lingonberry extract could reduce the apoptosis induced by PM2.5 with good dose-response relationship (r=-0.958, p < 0.05). The anti-apoptosis effect of bamboo leaf extract was higher than that of lingonberry extract at the same concentration (50 mg·L-1). The results suggested that the bamboo leaf extract and lingonberry extract would have no significant cyto-toxicity but have antagonistic effect on PM2.5-induced apoptotic rate in certain concentration range.
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