高级搜索

小麦细胞色素P450作为土壤污染生物标记物的研究

,  ,  ,  , 

downloadPDF

上一篇

下一篇

李昕馨, 宋玉芳, 杨道丽, 张薇, 宋雪英. 小麦细胞色素P450作为土壤污染生物标记物的研究[J]. 环境化学, 2006, 25(3): 283-287.
引用本文: 李昕馨, 宋玉芳, 杨道丽, 张薇, 宋雪英. 小麦细胞色素P450作为土壤污染生物标记物的研究[J]. 环境化学, 2006, 25(3): 283-287.
shu
LI Xin-xin, SONG Yu-fang, YANG Dao-li, ZHANG Wei, SONG Xue-ying. CYTOCHROME P450 IN WHEAT AS A BIOMARKER FOR DIAGNOSING POLLUTION IN SOIL[J]. Environmental Chemistry, 2006, 25(3): 283-287.
Citation: LI Xin-xin, SONG Yu-fang, YANG Dao-li, ZHANG Wei, SONG Xue-ying. CYTOCHROME P450 IN WHEAT AS A BIOMARKER FOR DIAGNOSING POLLUTION IN SOIL[J]. Environmental Chemistry, 2006, 25(3): 283-287.
shu

小麦细胞色素P450作为土壤污染生物标记物的研究

  • 1.

    中国科学院沈阳应用生态研究所陆地生态过程重点实验室, 沈阳, 110016;

  • 2.

    中国科学院研究生院, 北京, 100039;

  • 3.

    沈阳大学沈阳环境工程重点实验室, 沈阳, 110041;

  • 4.

    云南师范大学生命科学学院, 昆明, 650000

  • 基金项目:

    国家重点基础研究发展规划973项目(2004CB418503)

    国家自然科学基金项目(20337010,2057705,202770416)

    沈阳大学沈阳环境工程实验室项目

    中国科学院沈阳应用生态研究年海外留学基金

CYTOCHROME P450 IN WHEAT AS A BIOMARKER FOR DIAGNOSING POLLUTION IN SOIL

  • 1.

    Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China;

  • 2.

    Graduated School, Chinese Academy of Sciences, Beijing, 100039, China;

  • 3.

    Key Laboratory of Environment Engineering, University of Shenyang, Shenyang, 110041, China;

  • 4.

    College of Life Science, Yunnan Normal University, Kunming, 650000, China

  • Fund Project:

  • 摘要: 以小麦(Triticum acstivnm)为供试植物,建立了小麦细胞色素P450含量的测定方法.在此基础上,以草甸棕壤为供试土壤,菲为外源污染物,进行了菲污染暴露与P450含量的污染诱导量-效关系研究.比较了P450含量与超氧化物歧化酶(SOD)活性对污染诱导的敏感性.结果表明,当土壤菲浓度在1-8mg· kg-1范围内,P450总量分别为对照组的1.78,2.51,2.48和2.17倍,表现为诱导刺激效应,且P450含量与菲含量之间存在明显的剂量-效应关系(P=0.00).而同样浓度范围内,SOD活性变化与对照相比均无显著差异(P=0.168).
    Abstract: Wheat(Triticum acstivnm)as test plant was chosen to establish the method for determination of cytochrome P450 content,through selection of sampling site and quantification of CO charged.Relationships of dose-response for cytochrome P450 contents in wheat and phenanthrene concentrations were conducted in meadow brown soil.Changes in superoxide dismutase(SOD) activity were also evaluated to compare the sensibility with P450.Result of the contact test in soil for phenanthrene at different concentrations(1,2,4 and 8mg·kg-1) showed that there were a positive dose-response relationships between P450 and phenanthrene.Cytochrome P450 was induced significantly(P=0.00),and the inducing coefficients were 1.78,2.51,2.48 and 2.17 respectively.Within the same test concentrations,superoxide dismutase activity showed no obvious changes(P=0.168).
  • 加载中
  • [1] 冷欣夫,邱星辉,细胞色素P450酶系的结构、功能与应用前景.北京:科学出版社,2001,245-247
    [2] 朱琳,钱芸,刘广良,细胞色素P450酶系及其在毒理学上的应用.上海环境科学,2001,20(4):88-91
    [3] Galgani F,Bocquene G,Truquet Ph et al.,Monitoring of Pollutant Biochemical Effects on Marine Organisms of the French Coasts.Oceanologica ACTA,1992,15(4):52-61
    [4] 霍传林,王菊英,韩庚辰等,鱼体内EROD活性对多氯联苯类的指示作用.海洋环境科学,2002,21(1):5-8
    [5] 宋玉芳,周启星,宋雪英等,土壤环境污染的生态毒理学诊断方法研究进展.生态科学,2002,21(2):182-186
    [6] Omura T,Sato R,The Carbon Monoxide-binding Pigment of Liver Microsomes.J.Biol.Chem.,1964,239(7):2370-2378
    [7] Bradford M M,A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding.Anal.Biochem.,1976,72:248-254
    [8] Beauchamp C,Fridovich I,Superoxide Dismutase,Improved Assays and an Assay Applicable to Acrylamide Gels.Anal.Biochem.,1971,44:276-287
    [9] Song Y F,Jing X,Fleischmann S et al.,Comparative Study of Extraction Methods for the Determination of PAHs from Contaminated Soils and Sediments.Chemosphere,2002,48(9):993-1001
    [10] Durst F,Biochemistry and Physiology of Plant Cytochrome P450.In:Ruckpaul K,Rein H,eds.Microbial and Plant Cytochrome P450.London:Taylor and Francis,1991,191-233
    [11] 刘宛,李培军,周启星等,植物细胞色素P450酶系的研究进展及其与外来物质的关系.环境污染治理技术与设备,2001,2(5):1-9
    [12] Bolwell G P,Bozak K,Zimmerlin A,Plant Cytochrome P450.Phytochemistry,1994,37(6):1491-1506
    [13] 于彩虹,高烯武,棉铃虫细胞色素P450 CO差光谱的测定.昆虫学报,2005,48(2):301-304
    [14] 宋玉芳,周启星,宋雪英等,菲和芘对土壤中植物根伸长抑制的生态毒性效应.生态学杂志,2003,22(5):6-9
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  607
  • HTML全文浏览数:  607
  • PDF下载数:  384
  • 施引文献:  0
出版历程
李昕馨, 宋玉芳, 杨道丽, 张薇, 宋雪英. 小麦细胞色素P450作为土壤污染生物标记物的研究[J]. 环境化学, 2006, 25(3): 283-287.
引用本文: 李昕馨, 宋玉芳, 杨道丽, 张薇, 宋雪英. 小麦细胞色素P450作为土壤污染生物标记物的研究[J]. 环境化学, 2006, 25(3): 283-287.
shu
LI Xin-xin, SONG Yu-fang, YANG Dao-li, ZHANG Wei, SONG Xue-ying. CYTOCHROME P450 IN WHEAT AS A BIOMARKER FOR DIAGNOSING POLLUTION IN SOIL[J]. Environmental Chemistry, 2006, 25(3): 283-287.
Citation: LI Xin-xin, SONG Yu-fang, YANG Dao-li, ZHANG Wei, SONG Xue-ying. CYTOCHROME P450 IN WHEAT AS A BIOMARKER FOR DIAGNOSING POLLUTION IN SOIL[J]. Environmental Chemistry, 2006, 25(3): 283-287.
shu

小麦细胞色素P450作为土壤污染生物标记物的研究

  • 1.  中国科学院沈阳应用生态研究所陆地生态过程重点实验室, 沈阳, 110016;
  • 2.  中国科学院研究生院, 北京, 100039;
  • 3.  沈阳大学沈阳环境工程重点实验室, 沈阳, 110041;
  • 4.  云南师范大学生命科学学院, 昆明, 650000
基金项目:

国家重点基础研究发展规划973项目(2004CB418503)

国家自然科学基金项目(20337010,2057705,202770416)

沈阳大学沈阳环境工程实验室项目

中国科学院沈阳应用生态研究年海外留学基金

摘要: 以小麦(Triticum acstivnm)为供试植物,建立了小麦细胞色素P450含量的测定方法.在此基础上,以草甸棕壤为供试土壤,菲为外源污染物,进行了菲污染暴露与P450含量的污染诱导量-效关系研究.比较了P450含量与超氧化物歧化酶(SOD)活性对污染诱导的敏感性.结果表明,当土壤菲浓度在1-8mg· kg-1范围内,P450总量分别为对照组的1.78,2.51,2.48和2.17倍,表现为诱导刺激效应,且P450含量与菲含量之间存在明显的剂量-效应关系(P=0.00).而同样浓度范围内,SOD活性变化与对照相比均无显著差异(P=0.168).

English Abstract

    全文HTML

参考文献 (1)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心