粮食中玉米赤霉烯酮的电子捕获气相色谱分析

环境化学

粮食中玉米赤霉烯酮的电子捕获气相色谱分析

, ,

罗毅, 郑集声, 杨进生. 粮食中玉米赤霉烯酮的电子捕获气相色谱分析[J]. 环境化学, 1991, 10(1): 59-63.

引用本文:

罗毅, 郑集声, 杨进生. 粮食中玉米赤霉烯酮的电子捕获气相色谱分析[J]. 环境化学, 1991, 10(1): 59-63.

Luo Yi, Zheng Jisheng, Yang Jinsheng. DETERMINATION OF ZEARALENONE IN FOODSTUFFS AND FEEDSTUFFS BY GC WITH ELECTRON-CAPTURE DETECTOR[J]. Environmental Chemistry, 1991, 10(1): 59-63.

Citation:

Luo Yi, Zheng Jisheng, Yang Jinsheng. DETERMINATION OF ZEARALENONE IN FOODSTUFFS AND FEEDSTUFFS BY GC WITH ELECTRON-CAPTURE DETECTOR[J]. Environmental Chemistry, 1991, 10(1): 59-63.

粮食中玉米赤霉烯酮的电子捕获气相色谱分析

1.
军事医学科学院毒物药物研究所

DETERMINATION OF ZEARALENONE IN FOODSTUFFS AND FEEDSTUFFS BY GC WITH ELECTRON-CAPTURE DETECTOR

1.
Intitnte of Pharmacology and Toxicology, Academy of Military Medical Sciences

摘要: 本文建立了粮食中玉米赤霉烯酮的电子捕获气相色谱方法。粮食样品经氯仿-醋酸乙酯（1:1）提取后浓缩,浓缩液用NaOH提取,NaOH提取液经HCI调至pH为3.5左右,过Sep-pak C18柱。用乙腈洗脱柱上玉米赤霉烯酮,洗脱液浓缩至干,残渣用七氟丁酸酐衍生化后进行气相色谱分析。结果表明,玉米赤霉烯酮在电子捕获气相色谱上的最低检出限为4×10-11g,相关系数为0.9996。在玉米、小麦、大麦、大米中的回收率在50ng/g—100ng/g的范围内均在80%以上。各粮食中的最低检出限均低于20ng/g。

Abstract: A method to detect zearalenone in corn, wheat, rice and barley by gas-liquid chromatography with electron-capture detector was developed. Toxin was extracted with chloroform-ethyl acetate (1:1) and dissolved in aqueous NaOH solution. The solution was acidified to pH 3.5, passed through a Sep-pak C18 column,and eluted with acetonitrile. Zearalenone was detected quantitatively by GC after derivatized with heptafluorobutyric acid anhydride. Recovery of zearalenone from cereals was all above 80% at 50ng-100ng/g. The detection limit was less than 20ng/g.

WeChat

点击查看大图

计量

文章访问数: 592
HTML全文浏览数: 592
PDF下载数: 196
施引文献: 0

罗毅, 郑集声, 杨进生. 粮食中玉米赤霉烯酮的电子捕获气相色谱分析[J]. 环境化学, 1991, 10(1): 59-63.

引用本文:

罗毅, 郑集声, 杨进生. 粮食中玉米赤霉烯酮的电子捕获气相色谱分析[J]. 环境化学, 1991, 10(1): 59-63.

Luo Yi, Zheng Jisheng, Yang Jinsheng. DETERMINATION OF ZEARALENONE IN FOODSTUFFS AND FEEDSTUFFS BY GC WITH ELECTRON-CAPTURE DETECTOR[J]. Environmental Chemistry, 1991, 10(1): 59-63.

Citation:

Luo Yi, Zheng Jisheng, Yang Jinsheng. DETERMINATION OF ZEARALENONE IN FOODSTUFFS AND FEEDSTUFFS BY GC WITH ELECTRON-CAPTURE DETECTOR[J]. Environmental Chemistry, 1991, 10(1): 59-63.

粮食中玉米赤霉烯酮的电子捕获气相色谱分析

1. 军事医学科学院毒物药物研究所

收稿日期: 1989-02-03

摘要: 本文建立了粮食中玉米赤霉烯酮的电子捕获气相色谱方法。粮食样品经氯仿-醋酸乙酯（1:1）提取后浓缩,浓缩液用NaOH提取,NaOH提取液经HCI调至pH为3.5左右,过Sep-pak C18柱。用乙腈洗脱柱上玉米赤霉烯酮,洗脱液浓缩至干,残渣用七氟丁酸酐衍生化后进行气相色谱分析。结果表明,玉米赤霉烯酮在电子捕获气相色谱上的最低检出限为4×10-11g,相关系数为0.9996。在玉米、小麦、大麦、大米中的回收率在50ng/g—100ng/g的范围内均在80%以上。各粮食中的最低检出限均低于20ng/g。

English Abstract

全文HTML

参考文献 (0)

/

下载: 全尺寸图片幻灯片

分享

用微信扫码二维码

分享至好友和朋友圈

返回