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白腐菌对十溴联苯醚的酶促降解研究

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熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
引用本文: 熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
shu
XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
Citation: XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
shu

白腐菌对十溴联苯醚的酶促降解研究

  • 1.

    暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州, 510632

  • 基金项目:

    国家自然科学基金委-广东省联合基金重点项目(U0933002)

    国家自然科学基金项目(50978122)

    中央高校基本科研业务费专项资金资助项目(21611343)资助.

Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium

  • 1.

    Department of Environmental Engineering, Jinan University, Key Laboratory of Water/soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China

  • Fund Project:

  • 摘要: 考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg·L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30 ℃、pH 4—5、BDE-209浓度1 mg·L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.
    Abstract: Degradation of BDE-209 by the cell, extracellular and intracellular enzymes of P.chrysosporium was investigated, and after 7 days 60.17%, 54.14% and 22.32% BDE-209 was degraded respectively at an initial BDE-209 concentration of 1 mg·L-1, which demonstrated that BDE-209 was mainly degraded by extracellular enzymes. The effect of temperature, pH, and BDE-209 concentration on the degradation of BDE-209 was further studied. The results indicated that the optimal conditions of BDE-209 degradation were as follow: temperature 30 ℃, pH 4—5, BDE-209 concentration 1 mg·L-1.Infrared spectroscopy analysis showed that the degradation of BDE-209 was related to Br—C、CH2—O—CH2. The GC-MS chromatogram revealed that deprivation of Br occured during the degradation of BDE-209.
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  • 收稿日期:  2011-09-21
熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
引用本文: 熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
shu
XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
Citation: XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
shu

白腐菌对十溴联苯醚的酶促降解研究

  • 1. 暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州, 510632
  • 收稿日期:  2011-09-21
基金项目:

国家自然科学基金委-广东省联合基金重点项目(U0933002)

国家自然科学基金项目(50978122)

中央高校基本科研业务费专项资金资助项目(21611343)资助.

摘要: 考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg·L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30 ℃、pH 4—5、BDE-209浓度1 mg·L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.

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