白腐菌对十溴联苯醚的酶促降解研究

熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
引用本文: 熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
Citation: XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.

白腐菌对十溴联苯醚的酶促降解研究

  • 基金项目:

    国家自然科学基金委-广东省联合基金重点项目(U0933002)

    国家自然科学基金项目(50978122)

    中央高校基本科研业务费专项资金资助项目(21611343)资助.

Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium

  • Fund Project:
  • 摘要: 考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg·L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30 ℃、pH 4—5、BDE-209浓度1 mg·L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.
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  • [1] Mckinney M A,Dietz R, Sonne C, et al. Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenylether, and decabromodiphenylethane flame retardants in arctic marine-feeding mammals[J]. Environ Toxicol Chem,2011,30(7):1506-1514
    [2] Moller A, Xie Z Y, Sturm R, et al. Polybrominated diphenyl ethers (PBDEs) and alternative brominated flame retardants in air and seawater of the European Arctic[J]. Environ Pollut,2011,159(6):1577-1583
    [3] Qiu X H, Zhu T, Hu J X. Polybrominated diphenyl ethers (PBDEs) and other flame retardants in the atmosphere and water from Taihu Lake, East China[J]. Chemosphere, 2010, 80(10):1207-1212
    [4] Gandhi N, Bhavsar S P, Gewurtz S B, et al. Can biotransformation of BDE-209 in lake trout cause bioaccumulation of more toxic, lower-brominated PBDEs (BDE-47,-99) over the long term?[J]. Environ Int, 2011, 37(1):170-177
    [5] Puzyn T,Haranczyk M,Suzuki N,et al.Estimating persistence of brominated and chlorinated organic pollutants in air, water, soil, and sediments with the QSPR-based classification scheme[J]. Mol Divers, 2011, 15(1):173-188
    [6] Liang X W, Zhu S Z,Chen P, et al. Bioaccumulation and bioavailability of polybrominated diphynel ethers (PBDEs) in soil[J]. Environ Pollut,2010,158(7):2387-2392
    [7] Leung A O W, Luksemburg W J, Wong A S, et al. Spatial distribution of polybrominated diphenyl ethers and polychlorinated dibenzo-p-dioxins and dibenzofurans in soil and combusted residue at Guiyu, an electronic waste recycling site in southeast China[J]. Environ Sci Technol, 2007, 41(8): 2730-2737
    [8] Xia C H, Lam J C W, Wu X G, et al. Levels and distribution of polybrominated diphenyl ethers (PBDEs) in marine fishes from Chinese coastal waters[J]. Chemosphere, 2011, 82(1): 18-24
    [9] Yen J H, Liao W C, Chen W C, et al.Interaction of polybrominated diphenyl ethers (PBDEs) with anaerobic mixed bacterial cultures isolated from river sediment[J]. J Hazard Mater, 2009, 165(1/3):518-524
    [10] Shin M, Duncan B, Seto P, et al. Dynamics of selected pre-existing polybrominated diphenylethers (PBDEs) in municipal wastewater sludge under anaerobic conditions[J]. Chemosphere, 2010, 78(10):1220-1224
    [11] 王芳芳,尹华,龙焰,等. 表面活性剂对苏云金芽孢杆菌J-1降解BDE-209的影响[J]. 环境科学学报,2011,31(4):738-744
    [12] Robrock Kristin R,Coelhan Mehmet,Sedlak David L,et al. Aerobic biotransformation of polybrominated diphenyl ethers (PBDEs) by bacterial isolates[J]. Environ Sci Technol, 2009, 43(15):5705-5711
    [13] 苑宝玲,陈彩云,李云琴,等. 假单胞菌胞内酶粗提液对藻毒素MCLR的降解[J]. 环境化学,2009,28(6):854-858
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  • 收稿日期:  2011-09-21
熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
引用本文: 熊士昌, 尹华, 何宝燕, 彭辉, 龙焰, 叶锦韶, 张娜, 彭素芬. 白腐菌对十溴联苯醚的酶促降解研究[J]. 环境化学, 2012, 31(5): 615-619.
XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.
Citation: XIONG Shichang, YIN Hua, HE Baoyan, PENG Hui, LONG Yan, YE Jinshao, ZHANG Na, PENG Sufen. Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J]. Environmental Chemistry, 2012, 31(5): 615-619.

白腐菌对十溴联苯醚的酶促降解研究

  • 1. 暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州, 510632
基金项目:

国家自然科学基金委-广东省联合基金重点项目(U0933002)

国家自然科学基金项目(50978122)

中央高校基本科研业务费专项资金资助项目(21611343)资助.

摘要: 考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg·L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30 ℃、pH 4—5、BDE-209浓度1 mg·L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.

English Abstract

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