摘要:
由于传统固相萃取需耗费大量时间，使用很多有机溶剂，在样品量多的时候，会出现大量样品滞留，为提高实验室工作人员的工作效率，本文建立了在线固相萃取-超高效液相色谱法测定水中15种多环芳烃污染物的方法.水样经在线固相萃取小柱富集，高效液相色谱柱分离，乙腈和水溶液梯度洗脱，荧光检测器检测，外标法定量.在进样量为2 mL时，萘、二氢苊、芴、菲、蒽、荧蒽、芘、（口恶）、苯并（a）蒽、苯并（b）荧蒽、苯并（k）荧蒽、苯并（a）芘、二苯并（a，h）蒽、苯并（g，h，i）苝、茚并（1，2，3-cd）芘在5-80 ng·L-1范围内均具有良好的线性关系.实际水样回收率均大于70%，此方法可用于水中15种多环芳烃类污染物的检测.
Abstract:
The traditional solid-phase extraction has many disadvantages. It alwaystakes a lot of time and uses too many organic solvents. Besides that, when there are a large number of samples need to be solid-phase extraction, most of them will have the phenomenon of retention.To improve the working efficiency of the staff in laboratory,we provide a new technology of on-line solid phase extraction. This method can test 15 polycyclic aromatic hydrocarbon(PAHs) pollutants in water.The water is enriched by on-line solid phase extraction column, high performance liquid chromatography column separation, acetonitrile and aqueous solution gradient elution, fluorescence detector detection, and external standard method quantification. When the sample volume is 2mL,below samples will have a good linear relationship in the range of 5-80 ng·L-1 likenaphthalene, acenaphthene, fluorine, phenanthrene, anthraquinone, fluoranthene, pyrene, chrysene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(k)fluoranthene, Benzo (a) pyrene, dibenzo (a, h) anthracene, benzo (g, h, i) pyrene, indole (1,2,3-cd) pyrene.And alsothiswater sample recovery ratehasexceeded 80%. This method can be used to detect 15 kinds of polycyclic aromatic hydrocarbon pollutants in water.
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