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丙酯草醚降解菌CY的分离、鉴定及其降解特性

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蔡志强, 杨百科, 赵希岳, 陈巧丽, 王利群, 黄磊, 叶庆富. 丙酯草醚降解菌CY的分离、鉴定及其降解特性[J]. 环境化学, 2011, 30(8): 1433-1438.
引用本文: 蔡志强, 杨百科, 赵希岳, 陈巧丽, 王利群, 黄磊, 叶庆富. 丙酯草醚降解菌CY的分离、鉴定及其降解特性[J]. 环境化学, 2011, 30(8): 1433-1438.
shu
CAI Zhiqiang, YANG Baike, ZHAO Xiyue, CHEN Qiaoli, WANG liqun, HUANG Lei, YE Qingfu. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF A HERBICIDE ZJ0273-DEGRADING STRAIN CY[J]. Environmental Chemistry, 2011, 30(8): 1433-1438.
Citation: CAI Zhiqiang, YANG Baike, ZHAO Xiyue, CHEN Qiaoli, WANG liqun, HUANG Lei, YE Qingfu. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF A HERBICIDE ZJ0273-DEGRADING STRAIN CY[J]. Environmental Chemistry, 2011, 30(8): 1433-1438.
shu

丙酯草醚降解菌CY的分离、鉴定及其降解特性

  • 1.

    常州大学微生物与生物工程实验室, 常州, 213164;

  • 2.

    浙江大学, 原子核农业科学研究所, 农业部核农学重点开放实验室, 杭州, 310029

  • 基金项目:

    国家自然科学基金(面上项目10775118,重点项目20632070)

    中国科学院知识创新工程重要方向项目(KGCX3-SYW-203-03)

    农业部公益性行业科研专项(200803034)

    常州市科技基金项目(20092008)资助.

ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF A HERBICIDE ZJ0273-DEGRADING STRAIN CY

  • 1.

    Laboratory of Microbiology and Biotechnology, Changzhou University, Changzhou, 213164, China;

  • 2.

    Institute of Nuclear Agricultural Science/Key Lab. of Nuclear-Agricultural Science, Ministry of Agriculture, Zhejiang Uviversity, Hangzhou, 310029, China

  • Fund Project:

  • 摘要: 采用富集培养的方法,从施用过丙酯草醚除草剂的油菜田中分离筛选出5株以丙酯草醚(ZJ0273)为唯一生长碳源和能源的菌株,选其中降解率高的菌株CY进行研究.根据其生理生化和16S rRNA 基因序列相似性分析,鉴定其为芽孢杆菌属(Bacillus sp.).根据对CY的初步研究和简单优化,菌株CY在30 d内对50 mg·L-1 ZJ0273的降解率达到55.1%,该菌株降解ZJ0273的最适初始pH值为8.0,最适温度为35 ℃.菌株CY对ZJ0273的降解效果与初始接种量在一定范围内呈正相关,当初始浓度＞400 mg·L-1时,ZJ0273降解率偏低.
    Abstract: Propyl (4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino)benzoate(ZJ0273) is a new herbicide used in the rapeseed fields. Five strains which utilize ZJ0273 as sole carbon and energy source were isolated from ZJ0273-used soil in oilseed fields by selective enrichment culture, and a CY strain most capable of degrading ZJ0273 was selected. Based on its physiological and biochemical characteristics and phylogenetic analysis of 16S rRNA sequences, the strain was identified as Bacillus sp.. When the initial concentration of ZJ0273 was 50 mg·L-1 in the basic medium, the degradation rate of ZJ0273 within 30 days was 55.1% by strain CY. The optimal intial pH of culture medium and temperature were 8.0 and 35 ℃ respectively. The degradation rate of ZJ0273 by strain CY showed a positive correlation with inoculating rate within a certain range, and the degradation rate was lower when initial concentration of ZJ0273 was more than 400 mg·L-1.
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  • 收稿日期:  2010-10-15
蔡志强, 杨百科, 赵希岳, 陈巧丽, 王利群, 黄磊, 叶庆富. 丙酯草醚降解菌CY的分离、鉴定及其降解特性[J]. 环境化学, 2011, 30(8): 1433-1438.
引用本文: 蔡志强, 杨百科, 赵希岳, 陈巧丽, 王利群, 黄磊, 叶庆富. 丙酯草醚降解菌CY的分离、鉴定及其降解特性[J]. 环境化学, 2011, 30(8): 1433-1438.
shu
CAI Zhiqiang, YANG Baike, ZHAO Xiyue, CHEN Qiaoli, WANG liqun, HUANG Lei, YE Qingfu. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF A HERBICIDE ZJ0273-DEGRADING STRAIN CY[J]. Environmental Chemistry, 2011, 30(8): 1433-1438.
Citation: CAI Zhiqiang, YANG Baike, ZHAO Xiyue, CHEN Qiaoli, WANG liqun, HUANG Lei, YE Qingfu. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF A HERBICIDE ZJ0273-DEGRADING STRAIN CY[J]. Environmental Chemistry, 2011, 30(8): 1433-1438.
shu

丙酯草醚降解菌CY的分离、鉴定及其降解特性

  • 1.  常州大学微生物与生物工程实验室, 常州, 213164;
  • 2.  浙江大学, 原子核农业科学研究所, 农业部核农学重点开放实验室, 杭州, 310029
  • 收稿日期:  2010-10-15
基金项目:

国家自然科学基金(面上项目10775118,重点项目20632070)

中国科学院知识创新工程重要方向项目(KGCX3-SYW-203-03)

农业部公益性行业科研专项(200803034)

常州市科技基金项目(20092008)资助.

摘要: 采用富集培养的方法,从施用过丙酯草醚除草剂的油菜田中分离筛选出5株以丙酯草醚(ZJ0273)为唯一生长碳源和能源的菌株,选其中降解率高的菌株CY进行研究.根据其生理生化和16S rRNA 基因序列相似性分析,鉴定其为芽孢杆菌属(Bacillus sp.).根据对CY的初步研究和简单优化,菌株CY在30 d内对50 mg·L-1 ZJ0273的降解率达到55.1%,该菌株降解ZJ0273的最适初始pH值为8.0,最适温度为35 ℃.菌株CY对ZJ0273的降解效果与初始接种量在一定范围内呈正相关,当初始浓度＞400 mg·L-1时,ZJ0273降解率偏低.

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