2017 Volume 11 Issue 4
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WU Manli, SHI Yixin, LI Wei, YUAN Jing, HUANG Jin. High performance liquid chromatography coupling with photo-diode array and fluorescence detector for sensitive analysis of environmental endocrine disruptors (EDCs) in wastewater[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2614-2620. doi: 10.12030/j.cjee.201510228
Citation: WU Manli, SHI Yixin, LI Wei, YUAN Jing, HUANG Jin. High performance liquid chromatography coupling with photo-diode array and fluorescence detector for sensitive analysis of environmental endocrine disruptors (EDCs) in wastewater[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2614-2620. doi: 10.12030/j.cjee.201510228

High performance liquid chromatography coupling with photo-diode array and fluorescence detector for sensitive analysis of environmental endocrine disruptors (EDCs) in wastewater

  • Received Date: 29/01/2016
    Accepted Date: 29/10/2015
    Available Online: 22/04/2017
    Fund Project:
  • A sensitive method combining high-performance liquid chromatography and a photo-diode array and fluorescence detector was developed to quantitatively analyze five environmental endocrine disruptors (EDCs): estrone (E1), estradiol (E2), ethinyl estradiol (EE2), estriol (E3), and bisphenol A (BPA). The LOD of the five EDCs ranged from 4.30 μg·L-1 to 9.54 μg·L-1 and the LOQ ranged from 12.90 μg·L-1 to 28.62 μg·L-1. The intra- and interday precision were lower than 6.86% and 9.47%, respectively. The recovery of all compounds varied from 62.8% to 96.0%. This method can meet the requirements of trace analysis. Wastewater samples collected from wastewater treatment plants (WWTP) were tested according to this method. The results indicated that the total concentrations of five EDCs were 3 073.1 ng·L-1 in the influent of the WWTP. Among the five EDCs, the content of E3 was highest, accounting for 40.1% of total EDCs. Throughout the wastewater treatment process, different treatment units exhibited different removal efficiencies for the EDCs. The biological treatment stage appeared to be necessary for the effective removal of EDCs. The removal efficiencies of EDCs ranged from 73.6% to 96.6% in the biological treatment units. The final sedimentation and disinfection for EDC removal were minor.
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High performance liquid chromatography coupling with photo-diode array and fluorescence detector for sensitive analysis of environmental endocrine disruptors (EDCs) in wastewater

Fund Project:

Abstract: A sensitive method combining high-performance liquid chromatography and a photo-diode array and fluorescence detector was developed to quantitatively analyze five environmental endocrine disruptors (EDCs): estrone (E1), estradiol (E2), ethinyl estradiol (EE2), estriol (E3), and bisphenol A (BPA). The LOD of the five EDCs ranged from 4.30 μg·L-1 to 9.54 μg·L-1 and the LOQ ranged from 12.90 μg·L-1 to 28.62 μg·L-1. The intra- and interday precision were lower than 6.86% and 9.47%, respectively. The recovery of all compounds varied from 62.8% to 96.0%. This method can meet the requirements of trace analysis. Wastewater samples collected from wastewater treatment plants (WWTP) were tested according to this method. The results indicated that the total concentrations of five EDCs were 3 073.1 ng·L-1 in the influent of the WWTP. Among the five EDCs, the content of E3 was highest, accounting for 40.1% of total EDCs. Throughout the wastewater treatment process, different treatment units exhibited different removal efficiencies for the EDCs. The biological treatment stage appeared to be necessary for the effective removal of EDCs. The removal efficiencies of EDCs ranged from 73.6% to 96.6% in the biological treatment units. The final sedimentation and disinfection for EDC removal were minor.

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