2014 Volume 8 Issue 8
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Wang Zhigang, Ai Dehua, Zhang Ying, Wang Yang, Jiang Zhao, Xu Weihui. Screening and application of carrier materials of atrazine remediation microbial inoculum of polluted soil[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3487-3494.
Citation: Wang Zhigang, Ai Dehua, Zhang Ying, Wang Yang, Jiang Zhao, Xu Weihui. Screening and application of carrier materials of atrazine remediation microbial inoculum of polluted soil[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3487-3494.
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Screening and application of carrier materials of atrazine remediation microbial inoculum of polluted soil

  • 1.

    College of Life Science and Agriculture & Forestry, Qiqihar University, Qiqihar 161006, China

  • 2.

    College of Resource & Environment, Northeast Agricultural University, Harbin 150030, China

  • Received Date: 01/04/2014
    Accepted Date: 16/03/2014
    Available Online: 31/07/2014
    Fund Project:
  • Atrazine, a broad-leaf weed control herbicide, is popularly used in agriculture, but has a long residual timing in the environment. The environmental behaviors and bioremediation technology of atrazine pollution have become a hotspot in the pesticide pollution control research field. The kaolin, attapulgite and humic acid were used as the carrier materials in atrazine remediation by microbial inoculum. The mass ratio of the carrier materials was optimized by the orthogonal experimental method in which the survival rates were used as target properties and the balling rates of carrier materials were referenced. Three material ratios were screened for the best performance [1:0.5:0.5 (A3B2C1),0.5:0:0.5 (A2B3C1) and 1:0:1 (A3B1C2)]. Under the temperature and UV tolerance tests conducted on these three material ratios, results obtained indicated that A3B2C1 could effectively enhance the strain tolerance under these conditions. That is, the most effective mass ratio obtained for the three carrier materials of kaolin, attapulgite and humic acid was 1:0.5:0.5; utilizing A3B2C1 in preparation of bacteria strains for an indoor soil bioremediation research, results obtained after a 35 d experiment revealed complete atrazine degradation was achieved when 0.1% and 0.5% carrier agents were added. While atrazine residuals of the other two free bacteria remediation were observed to be above 16%. The result of Shannon index and evenness of soil microbial changes in the remediation with the additional 0.1% carrier agent was lowest compared with other remediations in the repair process, likewise it had the most beneficial soil microbial ecosystem balance. Hence, addition of 0.1% carrier agent was regarded as the best remediation method.
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Screening and application of carrier materials of atrazine remediation microbial inoculum of polluted soil

  • Received Date: 01/04/2014
  • Accepted Date: 16/03/2014
  • Available Online: 31/07/2014
Fund Project:

Abstract: Atrazine, a broad-leaf weed control herbicide, is popularly used in agriculture, but has a long residual timing in the environment. The environmental behaviors and bioremediation technology of atrazine pollution have become a hotspot in the pesticide pollution control research field. The kaolin, attapulgite and humic acid were used as the carrier materials in atrazine remediation by microbial inoculum. The mass ratio of the carrier materials was optimized by the orthogonal experimental method in which the survival rates were used as target properties and the balling rates of carrier materials were referenced. Three material ratios were screened for the best performance [1:0.5:0.5 (A3B2C1),0.5:0:0.5 (A2B3C1) and 1:0:1 (A3B1C2)]. Under the temperature and UV tolerance tests conducted on these three material ratios, results obtained indicated that A3B2C1 could effectively enhance the strain tolerance under these conditions. That is, the most effective mass ratio obtained for the three carrier materials of kaolin, attapulgite and humic acid was 1:0.5:0.5; utilizing A3B2C1 in preparation of bacteria strains for an indoor soil bioremediation research, results obtained after a 35 d experiment revealed complete atrazine degradation was achieved when 0.1% and 0.5% carrier agents were added. While atrazine residuals of the other two free bacteria remediation were observed to be above 16%. The result of Shannon index and evenness of soil microbial changes in the remediation with the additional 0.1% carrier agent was lowest compared with other remediations in the repair process, likewise it had the most beneficial soil microbial ecosystem balance. Hence, addition of 0.1% carrier agent was regarded as the best remediation method.

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