引用本文:
李晓敏, 胡莹, 黄益宗, 刘云霞, 李季. 应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响[J]. 环境化学, 2017, 36(8): 1724-1734
LI Xiaomin, HU Ying, HUANG Yizong, LIU Yunxia, LI Ji. Effects of cations and pH in nutrient solution on zinc toxicity to rice (Oryza sativa) root elongation by the biotic ligand model[J]. Environmental Chemistry, 2017, 36(8): 1724-1734

应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响
李晓敏1, 胡莹1, 黄益宗2, 刘云霞1, 李季1
1. 中国科学院生态环境研究中心, 北京, 100085;
2. 农业部环境保护科研监测所, 天津, 300191
摘要:
采用室内水培实验, 通过改变溶液中pH值及主要阳离子(Ca2+、Mg2+、Na+和K+)浓度研究锌对水稻的毒性,建立了Zn对水稻根伸长毒性的生物配体模型(Biotic ligand model,BLM).研究结果表明,增加Ca2+、Mg2+和H+的活度均可以减缓Zn2+对水稻根伸长的毒性,而增加Na+、K+的活度对Zn2+的毒性影响不大;在低pH(4.5-6.0)条件下,主要是Zn2+对水稻根伸长产生毒性,在高pH(6.5-8.0)条件下,Zn2+和ZnOH+是主要的致毒形态.根据生物配体模型理论估算的Zn2+、ZnOH+、Ca2+、Mg2+和H+的生物配体络合的平衡常数分别为:lgKZnBL=4.97、lgKZnOHBL=5.30、lgKCaBL=2.96、lgKMgBL=3.30和lgKHBL=5.21.根据各平衡常数计算可得,当Zn结合水稻的生物配体位点达到73%之后,水稻根伸长的抑制率达50%(即f50=73%).利用上述参数建立的生物配体模型预测的EC50值均在实测值的2倍变化范围之内,表明生物配体模型可以有效地预测锌对水稻根伸长的急性毒性.
关键词:    生物配体模型    阳离子    pH    水稻    Zn   
Effects of cations and pH in nutrient solution on zinc toxicity to rice (Oryza sativa) root elongation by the biotic ligand model
LI Xiaomin1, HU Ying1, HUANG Yizong2, LIU Yunxia1, LI Ji1
1. Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;
2. Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, 300191, China
Abstract:
Acute toxicity of zinc (Zn) to rice (Oryza sativa) root elongation was investigated by changing the pH value and major cation (Ca2+, Mg2+, Na+ and K+) concentrations to develop an appropriate biotic ligand model (BLM) in solution culture. The results showed that the toxicity of Zn2+ was reduced with increasing avtivities of Ca2+, Mg2+ and H+, while the avtivities of Na+ and K+ did not significantly affect the Zn2+ toxicity. The toxicity could be explained mainly by Zn2+ binding to a biotic ligand (BL) at pH from 4.5 to 6.0 and by the joint toxicity of Zn2+ and ZnOH+ at pH from 6.5 to 8.0. According to the biotic ligand model (BLM) concept, the conditional stability constants for the binding of Zn2+, ZnOH+, Ca2+, Mg2+ and H+ to the BL were lgKZnBL=4.97, lgKZnOHBL=5.30, lgKCaBL=2.96, lgKMgBL=3.30 and lgKHBL=5.21, respectively. It was calculated that on average 73% of BL sites were occupied by Zn2+ when the rice root elongation was inhibited by 50% (f50=73%). On the basis of these estimated parameters, the BLM developed in this study could predict EC50 within a factor of 2 of the observed EC50. It was implicated that Zn-BLM could predict reliably the toxicity of Zn to rice.
Key words:    biotic ligand model    cations    pH    rice    Zn   
收稿日期: 2017-03-20
基金项目: 国家自然科学基金(21377152)资助.
胡莹,Tel:010-62849158,E-mail:huying@rcees.ac.cn
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